hj_purohit [at] neeri [dot] res [dot] in
Designation: Chief Scientist
Qualification: Ph.D.(Micro), M.Sc.(Micro)
Specialization: Molecular Biology
Address: EBGD Division, NEERI, Nagpur

Staff Id:





Chief Scientist (Scientist G)


Ph.D. , M.Sc. , B.Sc.


Environmental Biotechnology Genomics Division (EBGD)



Contact Number Extension:

Dir L. Line : (0712) 2249878

Phone       :(0712) 2249883  Ext – 251

Fax  No.    :(0712) 2249900

Research area/ Specialization:

Molecular Biology

Major project handled:

Sr. No.

Project Name


Microbial diversity of human gut


Biomethanation of Lignocellulosic Residues- Rice Straw 


Redefining the treatment strategy and assessment of Microbial catabolic diversity using genomics tool 


Mechanistic understanding of disinfection using Bauxite Residue


Waste utilization and Management


Biogas from vegetable waste using baffled plug flow reactor system


Metagenomic insight to water biofilm formation and dispersal


Microbial consortia for improved CETP performance  


Microbial consortia and Biomolecules


Evaluation of Bacteriophage Diversity in STPs Using Genomic Tools


  • Kapley A, Liu R, Jadeja N, Zhang Y, Yang M, Purohit HJ (2015) Shifts in microbial community and its correlation with degradative efficiency in a wastewater treatment plant, Applied Biochemistry and Biotechnology (Accepted).
  • Puranik S, Purohit H J. (2015) Dynamic interactive events in gene regulation using E. coli dehydrogenase as a model. Functional & Integrative Genomics, 15(2):175-88.
  • Pal RR, Khardenavis AA, Purohit HJ. (2015) Identification and monitoring of nitrification and denitrification genes in Klebsiella pneumoniae EGD-HP19-C for its ability to perform heterotrophic nitrification and aerobic denitrification. Functional & Integrative Genomics, 15, 63-76.
  • Daiwale AP, Sivanesan S, Izzotti A, Bafna A, Naoghare PK, Arrigo A, Purohit HJ, Parmar D, Krishnamurthy K. (2015) Noncoding RNAs: Possible player in development of fluorosis. Biomed Research International, Article ID 274852, (In press).
  • Husain AA, Daginawala HF, Warke SR, Kalorey DR,  Kurkure NV, Purohit HJ, Taori GM, Kashyap RS. (2015)  Investigation of immune biomarkers using subcutaneous model of M. tuberculosis infection in BALB/c mice: A preliminary report. Immune Network Journal, 15(2): 83–90.
  • Nagdev KJ, Bhagchandani SP, Bhullar SS, Kapgate RC, Kashyap RS, Chandak NH, Daginawala HF, Purohit HJ, Taori GM. (2015) Rapid diagnosis and simultaneous identification of tuberculous and bacterial meningitis by a newly developed duplex polymerase chain reaction. Indian Journal of Microbiology, 55 (2):213-218.
  • Nagdev KJ, Kashyap RS, Bhullar SS, Purohit HJ, Taori GM, Daginawala HF. (2015) Comparison of real-time PCR and conventional PCR assay using IS6110 region of Mycobacterium tuberculosis for efficient diagnosis of tuberculous meningitis and pulmonary tuberculosis. Indian Journal of Biotechnology, 14, 94-100.
  • Paliwal V, Raju SC, Modak A, Phale PS, Purohit HJ. (2014)  Pseudomonas putida CSV86: A Candidate Genome for Genetic Bioaugmentation. PLoS One. 9 (1), e84000.
  • More RP, Mitra S, Raju SC, Kapley A, Purohit HJ. (2014) Mining and assessment of catabolic pathways in the metagenome of a common effluent treatment plant to induce the degradative capacity of biomass. Bioresoure Technology 153, 137-46.
  • Deshmukh R, Purohit HJ. (2014) siRNA mediated gene silencing in Fusarium sp. HKF15 for overproduction of bikaverin. Bioresource Technology 157, 365-386.
  • Jadeja NB, More RP, Purohit HJ, Kapley A. (2014) Metagenomic analysis of oxygenases from activated sludge. Bioresource Technology 165, 250-256.
  • Shekhawat SD, Jain RK, Gaherwar HM, Purohit HJ, Taori GM, Daginawala HF, Kashyap RS. (2014) Heat shock proteins: Possible biomarkers in pulmonary and extrapulmonary tuberculosis. Human Immunology, 75 (2), 151-8.
  • Nousiainen AO, Björklöf K, Sagarkar S, Mukherjee S, Purohit HJ, Kapley A, Jørgensen KS. (2014) Atrazine degradation in boreal non-agricultural subsoil and tropical agricultural soil. Journal Soil Sediments.
  • Deshmukh R, Mathew A, Purohit HJ. (2014) Characterization of antibacterial activity of bikaverin from Fusarium sp. HKF15. Journal of Bioscience and Bioengineering 117 (4), 443-448.
  • Kashyap RS, Nayak AR, Gaherwar HM, Husain AA, Shekhawat SD, Jain RK, Panchbhai MS, Raje DV, Purohit HJ, Taori GM, Daginawala HF. (2014) Latent TB Infection Diagnosis in Population Exposed to TB Subjects in Close and Poor Ventilated High TB Endemic Zone in India. PloS one 9 (3), e89524.
  • Sagarkar S, Bhardwaj P, Yadav TC, Qureshi A, Khardenavis A, Purohit HJ, Kapley A. (2014) Draft genome sequence of atrazine-utilizing bacteria isolated from Indian agricultural soil. Genome Announc. 2 (1), e01149-13.
  • Qureshi A, Itankar Y, Ojha R, Mandal M, Khardenavis A, Kapley A, Purohit HJ. (2014) Genome Sequence of Lactobacillus plantarum EGD-AQ4, Isolated from Fermented Product of Northeast India. Genome Announcement. 2 (1), e01122-13.
  • Kashyap RS, Bhullar SS, More RP, Puranik S, Purohit HJ, Taori GM, Daginawala HF. (2014) Genome Sequence of Mycobacterium tuberculosis C2, a Cerebrospinal Fluid Clinical Isolate from Central India. Genome Announcement, Aug 21;2(4).pii: e00842-14.doi: 10.1128/genomeA.00842-14.
  • Kashyap RS, Morey SH, Bhullar SS, Baheti NN, Chandak NH, Purohit HJ, Taori GM, Daginawala HF. (2014) Determination of Toll-like receptor induced cytokine profile in blood and cerebrospinal fluid of Chikungunya patients.  Neuroimmunomodulation, 21, 338-346.
  • Bhullar SS, Chandak NH, Baheti NN, Purohit HJ, Taori GM, Daginawala HF, Kashyap RS. (2013) Identification of an immunodominant epitope in glycoproteins B and G of herpes simplex viruses (HSVs) using synthetic peptides as antigens in assay of antibodies to HSV in herpes simplex encephalitis patients. Acta Virologica, 58(3):267-73.
  • Agarwal L, Qureshi A, Kalia VC, Kapley A, Purohit HJ, Singh RN. (2014) Arid ecosystem: Future option for carbon sinks using microbial community intelligence. Current Science, 106, 1357-1363.
  • Puranik S, Purohit HJ. (2014) Dependency of Cellular Decision Making in Physiology and Influence of Preceding Growth Conditions. Applied Biochemistry Biotechnology, 174(5):1982-1998.
  • Husain AA, Daginawala HF, Kalorey DR, Nayak AR, Purohit HJ, Taori GM, Kashyap RS. (2013) Proteomic Approach to Identify Surrogate Biomarkers for Evaluation of Efficacy after Prime Boost Vaccination. Indian Journal of Applied Research, 3 (4), 452–455.
  • Husain AA, Daginawala HF, Purohit HJ, Taori GM, Kashyap RS. (2013) Assessment of cellular immune response to culture filtrate antigens of M. tuberculosis culture using in vitro PBMC model. Prospects to new vaccine development. European Journal of Experimental Biology, 3(3), 35–42.
  • Jablonski MR, Shaligram S, Qureshi A, Purohit HJ, Reisel JR. (2013) Degradation kinetics of resorcinol by Enterobacter cloacae isolate. African Journal of Microbiology Research, 7(28), 3632–3640.
  • Jain RK, Nayak AR, Husain AA, Panchbhai MS, Chandak N, Purohit HJ, Taori GM, Daginawala HF, Kashyap RS. (2013) Mycobacterial dormancy regulon protein Rv2623 as a novel biomarker for the diagnosis of latent and active tuberculous meningitis. Disease Markers, 35 (5), 311–6.
  • Kashyap RS, Nayak AR, Gaherwar HM, Bhullar SS, Husain A a, Shekhawat SD, Jain RK, Gaikwad SS, Satav AR, Purohit HJ, Taori GM, Daginawala HF. (2013) Laboratory investigations on the diagnosis of tuberculosis in the malnourished tribal population of melghat, India. PLoS One 8 (9), e74652.
  • Kashyap RS, Nayak AR, Husain AA, Gaherwar HM, Purohit HJ, Taori GM, Daginawala HF. (2013) Tuberculosis in India : the continuing challenge.  Current Science 105 (5), 597-606.
  • Bhushan A, Joshi J, Shankar P, Kushwah J, Raju SC, Purohit HJ, Kalia VC. (2013) Development of Genomic Tools for the Identification of Certain Pseudomonas up to Species Level. Indian journal of Microbiology 53(3), 253-263.
  • Nayak AR, Kashyap RS, Kabra D, Purohit HJ, Taori GM, Daginawala HF. (2013) Prognostic evaluation of sex difference in adenosine deaminase and homocysteine activities of acute ischemic stroke patients. Basic and Clinical Sciences 2 (1), 25-29.
  • Bhullar SS, Chandak NH, Purohit HJ, Taori GM, Daginawala HF, Kashyap RS. (2013) Determination of Viral Load by Quantitative Real-Time PCR in Herpes Simplex Encephalitis Patients. Intervirology 57 (1), 1-7.
  • Puranik S, Talkal R, Qureshi A, Khardenavis A, Kapley A, Purohit HJ. (2013) Genome Sequence of the Pigment-Producing Bacterium Pseudogulbenkianiaferrooxidans, Isolated from Loktak Lake. Genome Announcement 1 (6), e01115–13.
  • Kapley A, Sagarkar S, Tanksale H, Sharma N, Qureshi A, Khardenavis A, Purohit HJ. (2013) Genome Sequence of Alcaligenes sp. Strain HPC1271., Genome Announcement, 1(1): doi:pii: e00235-12.
  • Phale PS, Paliwal V, Raju SC, Modak A, Purohit HJ. (2013) Genome Sequence of Naphthalene-Degrading Soil Bacterium Pseudomonas putida CSV86. Genome Announcement. 1(1). doi:pii: e00234-12.
  • Agarwal L, Purohit HJ.(2013) Genome Sequence of Rhizobium lupini HPC(L) Isolated from Saline Desert Soil, Kutch (Gujarat)., Genome Announcement. 1(1). doi:pii: e00071-122013.
  • Sagarkar S, Mukherjee S, Nousiainen A, Björklöf K, Purohit HJ, Jørgensen KS, Kapley A. (2013) Monitoring bioremediation of atrazine in soil microcosms using molecular tools.  Environmental Pollution 172:108-15.
  • Khardenavis AA, Wang JY, Ng WJ, Purohit HJ, (2013) Management of various organic fractions of municipal solid waste via recourse to VFA and biogas generation,  Environmental Technology, 34(13,14), 2085-2097.  
  • Kashyap RS, Shekhawat SD, Nayak AR, Purohit HJ, Taori GM, Daginawala HF., (2013) Diagnosis of tuberculosis infection based on synthetic peptides from Mycobacterium tuberculosis antigen 85 complex. Clinical Neurology and Neurosurgery 115(6):678-83.
  • Chourasia SR, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF., (2013) Effect of Aqueous Extract and Fractions of Fagonia arabica on In Vitro Anticoagulant Activity. Clinical and Applied Thrombosis/Homeostasis. DOI: 10.1177/1076029613491458.
  • Puranik S, Shaligram S, Paliwal V, Raje DV, Kapley A, Purohit HJ. (2012) Demonstration of sequential adaptation strategy for developing salt tolerance in bacteria for wastewater treatment: A study using Escherichia coli as model.     Bioresour Technol. 121C:282-289.
  • Deshmukh R and Purohit HJ. (2012) Peptide Scaffolds: Flexible Molecular Structures With Diverse Therapeutic Potentials, International Journal of Peptide Research and Therapeutics, 18:125–143.
  • Chavhan SK ,Kalorey DR, Nagdive AA, Purohit HJ, Barbuddhe SB, Kurkure NV. (2012) Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk, Tropical Animal Health Production, 44(2):247-52.
  • Paliwal V, Puranik S, Purohit HJ. (2012) Integrated perspective for effective bioremediation, Applied Biochemistry and Biotechnology, 166(4):903-24.
  • Patel SKS, Singh M, Kumar P, Purohit HJ, Kalia VC. (2012) Exploitation of defined bacterial cultures for production of hydrogen and polyhydroxybutyrate from pea-shells. Biomass and Bioenergy, 36: 218-225.
  • Chandra R, Bharagava RN, Kapley A, Purohit HJ. (2012) Characterization of Phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants. Bioresource Technology 103: 78–86.
  • Shrivastava R, Purohit HJ, Phale PS. (2011) Metabolism and Preferential Utilization of Phenylacetic acid and4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86. Journal of Bioremediation and Biodegradation 2:2.
  • Husain AA, Kashyap RS, Kalorey DR, Warke SR, Purohit HJ, Taori GM, Daginawala HF. (2011) Effect of repeat dose of BCG vaccination on humoral response in mice model. Indian Journal of Experiemental Biology. 49(1):7-10.
  • Nayak AR, Kashyap RS, Kabra D, Deoras P, Purohit HJ, Taori GM, Daginawala HF. (2011) Evaluation of routinely performed hematological and biochemical parameters for the prognosis of acute ischemic stroke patients. Neurological Sciences, 32(5):855-60.
  • Bhullar SS, Kashyap RS, Chandak NH, Purohit HJ, Taori GM, Daginawala HF. (2011) Protein A-Based ELISA: Its Evaluation in the Diagnosis of Herpes Simplex Encephalitis. Viral Immunology. 24: 341-346.
  • Kalia VC and Purohit HJ. (2011) Quenching the quorum sensing system: potential antibacterial drug targets, Critical Reviews in Microbiology, 37(2): 121-40.
  • Huma N, Shankar P, Kushwah J, Bhushan A, Joshi J, Mukherjee T, Raju SC, Purohit HJ and Kalia VC. (2011) Diversity and Polymorphism in AHL-lactonase Gene (aiiA) of Bacillus.  Journal of Microbiology and Biotechnology 21(10): 1001-1011.
  • Kalia VC, Raju SC, Purohit HJ. (2011) Genomic Analysis Reveals Versatile Organisms for Quorum Quenching Enzymes: Acyl-Homoserine Lactone-Acylase and –Lactonase, The Open Microbiology Journal, 5:1-13.
  • Mohan SV, Agarwal L, Mohanakrishna G, Srikanth S, Kapley A,                         Purohit HJ, Sharma PN, (2011) Firmicutes with iron dependent hydrogenase drive hydrogen production in anaerobic bioreactor using distillery wastewater.
  • International Journal of Hydrogen Energy, 36: 8234-8342.
  • Selvakumaran S, Kapley A, Kashyap SM, Daginawala HF, Kalia VC, Purohit HJ. (2011) Diversity of aromatic ring-hydroxylating dioxygenase gene in Citrobacter. Bioresource Technology 102: 4600-9,
  • Nagdev KJ, Kashyap RS, Parida MM, Kapgate RC, Purohit HJ, Taori GM, Daginawala HF. (2011) Loop-mediated isothermal amplification for rapid and reliable diagnosis of TBM. Journal Clinical Microbiology, 49(5): 1861-5.    
  • Chourasia SR, Kashyap RS, Purohit HJ, Deopujari JY, Taori GM, Daginawala HF. (2011) In-vitro clot lytic potential of Fagonia arabica: a comparative study of two methods. Blood Coagulation and Fibrinolysis, 22: 288-94.
  • Chandra R, Bharagava RN, Kapley A, Purohit HJ. (2011) Bacterial diversity, organic pollutants and their metabolites in two aeration lagoons of common effluent treatment plant (CETP) during the degradation and detoxification of tannery wastewater.                                                                                                           Bioresource Technology, 102: 2333-41.
  • Bangar MA, Shirale DJ, Purohit HJ, Chen W, Myung NV, Mulchandani A. (2011) Single Conducting Polymer Nanowire Based Sequence-Specific,Base-Pair-Length Dependant Label-free DNA Sensor. Electroanalysis, 23: 371-379.
  • Verma V, Raju SC, Kapley A, Kalia VC, Kanade GS, Daginawala HF, Purohit HJ. (2011) Degradative potential of Stenotrophomonas strain HPC383 having genes homologous to dmp operon. Bioresource Technology, 102: 3227-33.
  • Rajan AS, Kashyap RS, Shekhawat SD, Husain AA, Purohit HJ, Taori GM, Daginawala HF (2011) Relationship between hsp-65 and il-2, il-10 profile in tb patients. Implication for vaccine design: a preliminary study. IIOAB Journal 3 (1), 39-43.
  • Panchbhai MS, Kashyap RS, Purohit HJ, Taori GM, Daginawala HF. (2011). Evaluation of Quantiferon TB Gold Test for the Diagnosis of Latent and Active Tuberculosis. International Journal of Biology 3 (1), 122-129.
  • Nayak AR, Kashyap RS, Kabra D, Purohit HJ, Taori GM, Daginawala HF. (2011). Prognostic significance of ischemia-modified albumin in acute ischemic stroke patients: A preliminary st Annals of Neurosciences 18 (1), 5-7.
  • Kashyap RS, Saha SM, Nagdev KJ, Kelkar SS, Purohit HJ, Taori GM, Daginawala HF. (2010) Diagnostic markers for tuberculosis ascites: a preliminary study. Biomark Insights, 5:87-94.
  • Raje DV, Purohit HJ, Badhe YP, Tambe SS and B D Kulkarni. (2010) Self-Organizing maps: A tool to ascertaion taxonomic relatedness beased on features derived from 16S rDNA sequences. Journal of Biosciences, 35, 617-627.
  • Kashyap RS, Morey SH, Ramteke SS, Chandak NH, Parida M, Deshpande PS, Purohit HJ, Taori GM, Daginawala HF. (2010) Diagnosis of Chikungunya fever in an Indian population by an indirect enzyme-linked immunosorbent assay protocol based on an antigen detection assay: a prospective cohort study. Clinical Vaccine Immunology. 17(2):291-7.
  • Verma V, Raju SC , Kapley A , Kalia VC , Daginawala HF, Purohit HJ. (2010) Evaluation of genetic and functional diversity of Stenotrophomonas isolates from diverse effluent treatment plants, Bioresource Technology 101, 7744-7753.
  • Nagdev KJ, Kashyap RS, Deshpande PS, Purohit HJ, Taori GM, Daginawala HF. (2010) Comparative evaluation of a PCR assay with an in-house ELISA method for diagnosis of Tuberculous meningitis. Medical Science Monitor Basic Research 16, CR289-95.
  • Nagdev KJ, Kashyap RS, Deshpande PS, Purohit HJ, Taori GM, Daginawala HF (2010) Determination of polymerase chain reaction efficiency for diagnosis of tuberculous meningitis in Chelex-100((R)) extracted DNA samples. International Journal of Tuberculosis and Lung Disease 14: 1032-8.
  • Morey SH, Kashyap RS, Purohit HJ, Taori GM, Daginawala H. (2010) An approach towards peptide-based antibody detection for diagnosis of Chikungunya infection. Biomarkers 15(6):546-52.
  • Patel SKS, Purohit HJ, Kalia VC. (2010) Dark fermentative hydrogen production by defined mixed microbial cultures immobilized on ligno-cellulosic waste materials. International Journal of Hydrogen & Energy, 35: 10674- 10681.
  • Dafale N, Agrawal L , Kapley A, Meshram S, Purohit HJ, Wate S. (2010) Selection of indicator bacteria based on screening of 16S rDNA metagenomic library from a two-stage anoxic–oxic bioreactor system degrading azo dyes. Bioresource Technology, 101(2):476-84.
  • Kashyap RS, Husain AA, Morey SH, Panchbhai MS, Deshpande PS, Purohit HJ, Taori GM, Daginawala HF. (2010) Assessment of immune response to repeat stimulation with BCG vaccine using in vitro PBMC model, Journal of Immune Based Therapies and Vaccines 2010, 8:3.
  • Kashyap RS, Purohit HJ, Taori GM, Daginawala HF. (2010) Changes in Cerebrospinal fluid Cytokine expression in Tuberculous Meningitis patients with Treatment. Neuro Immuno Modulation 17: 333-339.
  • Khardenavis AA, Kapley A, Purohit HJ. (2010) Salicylic Acid Mediated Enhanced Biological Treatment of Wastewater. Applied Biochemistry and Biotechnology, 160(3):704-18.
  • Kashyap RS, Ramteke SS, Gaherwar HM, Deshpande PS, Purohit HJ, Taori GM, Daginawala H. (2010). Evaluation of BioFM liquid medium for culture of cerebrospinal fluid in tuberculous meningitis to identify Mycobacterium tuberculosis.  Indian J Med Microbiol. 28 (4), 366-9.
  • Kashyap RS, Morey SH, Chandak NH, Purohit HJ, Taori GM, Daginawala HF. (2010) Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications.    Cerebrospinal Fluid Res. 7 (12), 1-6.
  • Kashyap RS, Kabra DP, Nayak AR, Mishra RN, Deshpande SK, Karandikar PN, Purohit HJ, Taori GM, Daginawala HF. (2010) Protein Electrophoretogram in Serum of Acute Ischemic Stroke Patients & Its Correlation with S-100 ββ and Neuron Specific Enolase Level: A Pilot Study Annals of Neurosciences 13 (2), 36-40, 2010