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Atomic Absorption Spectrophotometers : GBC 904AA and Hitachi Z8000

Atomic Absorption Spectrophotometers : GBC 904AA and Hitachi Z8000

Atomic Absorption Spectrometer

(Perkin Elmer,AAnalyst 800, PinAAcle 900F)

Principle

  • Absorption of radiant energy by free atoms in ground state
  • Light source used to emit characteristic energy known as Hollow cathode Lamp
  • The absorption is proportional to the concentration of free atoms follows the Lambert-Beer law

          Absorbance = log10 Io/It = K.C.L.

In practical the Lambert-Beer's law can be reduced to

          Absorbance = log10 Io/It µ C

Applications

  • Determination of trace element in all types of environmental, biological and food samples.
  • Non-volatile metals viz., Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn are analysed in flame system and
  • Volatile metals viz., As, Se, etc are analysed using hydride generation accessory (FIAS) , graphite furnace (GAAS)