Submitted by administrator on Mon, 06/01/2015 - 17:54
Fluorescence Spectrophotometer : Hitachi F-4000
Fluorescence is a type of photoluminescence in which a molecule is promoted to an electronically excited state by absorption of UV/Visible / near IR radiation. The excited molecule then decays back to the ground state or lower-lying excited electronic states by emission of light. The emitted light is detected as an Fluorescence.
- Interfaced to a Personal Computer (PC) through standard interface, General Purpose Interface Bus (GPIB).
- Self-deozonating Xenon discharge lamp of 150 Watts is used as a high intensity excitation source.
- Monochromators were large-aperture stigmatic concave diffraction grating type with 900 lines/mm covering wave length range of 200 – 900 nm on both Excitation and Emission sides with maximum wavelength scan speed of 30000 nm/min.
- Quantification of aromatic or highly unsaturated organic molecules
- DNA sequencing
- Studies of ligand binding in biological systems
- In-situ analysis in biological systems & cell sorting
- Environmental remote sensing (hydrologic, aquatic & atmospheric)