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High Performence Liquid Chromatographs : Waters 204 and 501, Shimadzu LC-10

High Performence Liquid Chromatographs : Waters 204 and 501, Shimadzu LC-10

 

Principle

HPLC is a form of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector.

Theory

Compounds are separated by injecting a sample mixture onto the column. The different component in the mixture pass through the column at differentiates due to differences in their partition behavior between the mobile phase and the stationary phase. The mobile phase must be degassed to eliminate the formation of air bubbles.

The sample is injected into the injector with syringe and compounds are separated in the column which are detected by the detector and recorded by computer.

Block Diagram

Application

  • Analyzing air and water pollutants
  • Monitoring of pharmaceutical drugs and products.
  • Monitoring pesticide levels in the environment.
  • Estimation of food contaminants etc.