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Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes(3Biotech)
(VV Gujar, P Fuke, AA Khardenavis, HJ Purohit
,8 2, ,,106,Year : 2018)
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In this study, we have characterized a novel set of extracellular enzymes produced by Penicillium chrysogenum strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with Penicillium rubens, Penicillium chrysogenum followed by Penicillium expansum and Penicillium roqueforti. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.
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Recent advancements in the mitigation of obnoxious nitrogenous gases(Journal of Environmental Management)
(Ishan Raj, AN Vaidya, RA Pandey, Amit Bansiwal, Sharvari Deshmukh, Hemant J Purohit
,205, ,,319-336,Year : 2018)
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Nitrogenous gaseous emissions commonly have an obnoxious odor associated with it, which when discharged into the environment results in serious environmental problems and health hazards. Several strategies for mitigation of nitrogenous odorants have been reported which include physical, chemical and biological methods. Biological treatments are widely employed because of their efficiency even at low concentration, where physical and chemical methods are not effective. Most commonly used biological treatment methods are biofiltration, biotrickling filters and membrane bioreactors with innovative reactor design, mixing pattern, and air sparging, for example FEBR, ALR, etc. These treatment methods require a critical assessment for the mitigation of obnoxious nitrogen emissions, especially in the context of environmental protection. This review offers a critical evaluation of treatment methods for the mitigation of nitrogenous odorous compound with a key emphasis on biological treatment systems. Also, various mathematical modelling techniques required for optimized operation of biotreatment systems has been discussed.
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Control of Multi-drug Resistant Gene Flow in Environment through Bacteriophage Intervention(Applied Biochemistry and Biotechnology)
(Krupa M. Parmar, Zubeen J. Hathi, Nishant A. Dafale,181(3),,1007-1029,Year : 2017)
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The spread of multidrug-resistant (MDR) bacteria is an emerging threat to the environment and public wellness. Inappropriate use and indiscriminate release of antibiotics in the environment through un-metabolized form create a scenario for the emergence of virulent pathogens and MDR bugs in the surroundings. Mechanisms underlying the spread of resistance include horizontal and vertical gene transfers causing the transmittance of MDR genes packed in different host, which pass across different food webs. Several controlling agents have been used for combating pathogens; however, the use of lytic bacteriophages proves to be one of the most eco-friendly due to their specificity, killing only target bacteria without damaging the indigenous beneficial flora of the habitat. Phages are part of the natural microflora present in different environmental niches and are remarkably stable in the environment. Diverse range of phage products, such as phage enzymes, phage peptides having antimicrobial properties, and phage cocktails also have been used to eradicate pathogens along with whole phages. Recently, the ability of phages to control pathogens has extended from the different areas of medicine, agriculture, aquaculture, food industry, and into the environment. To avoid the arrival of pre-antibiotic epoch, phage intervention proves to be a potential option to eradicate harmful pathogens generated by the MDR gene flow which are uneasy to cure by conventional treatments.
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Evaluation, application and aptimization of advanced oxidation process for pretreatment of rice straw and its effect on cellulose digestibility(Renewable Energy)
("Morone , A.;
Sharma, G.;
Sharma, A.;
Chakrabarti, T.;
Pandey, R. A.
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,120,,88-97,Year : 2017)
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No information is available
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Mixotrophic cultivation of microalgae to enhance the quality of lipid for biodiesel application: effects of scale of cultivation and light spectrum on reduction of alpha- linolenic acid(Bioprocess and Biosystems Engineering)
(Gupta, S;
Pawar, SB
,41 (4),,531- 542,Year : 2017)
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No information is available
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Genomic characterization of key bacteriophages to formulate the potential biocontrol agent to combat enteric pathogenic bacteria(Archives of microbiology)
(Parmar, KM;
Dafale, NA;
Tikariha, H;
Purohit, HJ
,200 (4),,611- 622,Year : 2017)
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No information is available
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Heavy metal (Pb) accumulation in metallophytes as influenced by the variations in rhizospheric and non- rhizospheric soils physico-chemical characteristics(International Journal of Phytoremediation)
(Adejumo, SA;
Tiwari, S;
Shinde, V;
Sarangi, BK
,20 (3),,237- 248,Year : 2017)
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No information is available
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Genomic Tools for the Impact Assessment of 'Hotspots' for Early Warning of MDR Threats.(Biomedical and Environmental Science)
(Nishant Dafale, Hemat Purohit ,29(8),,143-147,Year : 2016)
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No information is available
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Insights in Waste Management Bioprocesses using Genomic Tools(Advances in Applied Microbiology)
(H. J. Purohit, A. Kapley, A. Khardenavis, A. Qureshi, Nishant A. Dafale,97,,121-170,Year : 2016)
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Microbial capacities drive waste stabilization and resource recovery in environmental friendly processes. Depending on the composition of waste, a stress-mediated selection process ensures a scenario that generates a specific enrichment of microbial community. These communities dynamically change over a period of time while keeping the performance through the required utilization capacities. Depending on the environmental conditions, these communities select the appropriate partners so as to maintain the desired functional capacities. However, the complexities of these organizations are difficult to study. Individual member ratios and sharing of genetic intelligence collectively decide the enrichment and survival of these communities. The next-generation sequencing options with the depth of structure and function analysis have emerged as a tool that could provide the finer details of the underlying bioprocesses associated and shared in environmental niches. These tools can help in identification of the key biochemical events and monitoring of expression of associated phenotypes that will support the operation and maintenance of waste management systems. In this chapter, we link genomic tools with process optimization and/or management, which could be applied for decision making and/or upscaling. This review describes both, the aerobic and anaerobic, options of waste utilization process with the microbial community functioning as flocs, granules, or biofilms. There are a number of challenges involved in harnessing the microbial community intelligence with associated functional plasticity for efficient extension of microbial capacities for resource recycling and waste management. Mismanaged wastes could lead to undesired genotypes such as antibiotic/multidrug-resistant microbes.
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Selection of appropriate analytical tools to determine the potency, microbial bioactivity and resistance of antibiotics(Journal of Pharmaceutical Analysis)
(Nishant Dafale, Uttam Semwal, Rupak Rajput, G. N. Singh ,6(4),,207-213 ,Year : 2016)
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Antibiotics are the chemotherapeutic agents that kill or inhibit the pathogenic microorganisms. Resistance of microorganism to antibiotics is a growing problem around the world due to indiscriminate and irrational use of antibiotics. In order to overcome the resistance problem and to safely use antibiotics, the correct measurement of potency and bioactivity of antibiotics is essential. Microbiological assay and high performance liquid chromatography (HPLC) method are used to quantify the potency of antibiotics. HPLC method is commonly used for the quantification of potency of antibiotics, but unable to determine the bioactivity; whereas microbiological assay estimates both potency and bioactivity of antibiotics. Additionally, bioassay is used to estimate the effective dose against antibiotic resistantmicrobes. Simultaneously, microbiological assay addresses the several parameters such as minimal inhibitory concentration (MIC), minimum bactericidal concentration (MBC), mutation prevention concentration (MPC) and critical concentration (Ccr) which are used to describe the potency in a more informative way. Microbiological assay is a simple, sensitive, precise and cost effective method which gives reproducible results similar to HPLC. However, the HPLC cannot be a complete substitute for microbiological assay and both methods have their own significance to obtain more realistic and precise results.
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An Insight into Diversity at Environmental Habitats using Comparative Metagenomics Approach(Current Microbiology)
(Parmar, K.;
Dafale, N.;
Pal, R.;
Tikariha, H,75(2),,132- 141,Year : 2016)
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No information is available
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Computational fluid dynamics (CFD) analysis of airlif bioreactor : effect of draft tube configuration on hydrodynamics, cell suspension, and shear rate(Bioprocess and Biosystems Engineering)
(Pawar, S. B.
,41(1),,31-45,Year : 2016)
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No information is available
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Evaluation of biogas production potential of kitchen waste in the presence of spices(Waste Management)
("Sahu, N;
Sharma, A.;
Mishra, P.;
Chandrashekhar, B.;
Sharma, G.;
Kapey, A.;
Pandey, R. A.
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,10,,236-246,Year : 2016)
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No information is available
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Bioremediation and detoxification of industrial wastes by earthworms : Vermicompost as powerful crop nutrient in sustainable agriculture(Bioresource Technology)
(Bhat, S. A.;
Singh, S.;
Singh, J.;
Bhawana, Vig, A. P.
,252 ,,172- 179,Year : 2016)
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No information is available
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Transformation Products of Carbamazepine (CBZ) After Ozonation and their Toxicity Evaluation Using Pseudomonas sp. Strain KSH-1 in Aqueous Matrices(Indian Journal of Microbiology)
(Dwivedi, K.;
Rudrashetti, A. P.;
Chakrabarti, T.;
Pandey, R.A.
,58(2),,190- 200,Year : 2016)
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No information is available
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Integrated bio-oxidation and adsorptive filtration reactor for removal of arsenic from wastewater(Environmental Technology (United Kingdom))
(Kamde, K;
Dahake, R;
Pandey, R. A;
Bansiwal, A.
,,,,Year : 2016)
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No information is available
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Development and validation of microbial bioassay for quantification of Levofloxacin in pharmaceutical preparations(Journal of Pharmaceutical Analysis)
(Nishant Dafale, Uttam Semwal, Piyush Agarwal, Pradip Sharma, G. N. Singh ,51,,18-26,Year : 2015)
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The aim of this study was to develop and validate a simple, sensitive, precise and cost-effective one-level agar diffusion (5+1) bioassay for estimation of potency and bioactivity of Levofloxacin in pharmaceutical preparation which has not yet been reported in any pharmacopoeia. Among 16 microbial strains, Bacillus pumilus ATCC-14884 was selected as the most significant strain against Levofloxacin. Bioassay was optimized by investigating several factors such as buffer pH, inoculums concentration and reference standard concentration. Identification of Levofloxacin in commercial sample Levoflox tablet was done by FTIR spectroscopy. Mean potency recovery value for Levofloxacin in Levoflox tablet was estimated as 100.90%. A validated bioassay method showed linearity (r2=0.988), precision (Interday RSD=1.05%, between analyst RSD=1.02%) and accuracy (101.23%, RSD=0.72%). Bioassay was correlated with HPLC using same sample and estimated potencies were 100.90% and 99.37%, respectively. Results show that bioassay is a suitable method for estimation of potency and bioactivity of Levofloxacin pharmaceutical preparations.
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Genome Annotation and Validation of Keratin-Hydrolyzing Proteolytic Enzymes from Serratia marcescens EGD-HP 20(Applied Biochemistry and Biotechnology)
(Fuke, P.;
Gujar, V. V.;
Khardenavis, A. A
,184(3),,970- 986,Year : 2015)
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No information is available
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Screening bamboo species for salt tolerance using growth parameters , physiological response and osmolytes accumulation as effective indicators(Chemistry and Ecology)
(Pulavarty, A.;
Sarangi, B. K,344,,340- 354,Year : 2015)
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No information is available
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Mixotrophic cultivation of microalge to enhance the quality of lipid for biodiestion of cow dung mixed with food waste.(Bioprocess and Biosystems Engineering)
("Gupta, S.;
Pawar, S. B.
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,41(4),,531- 542,Year : 2015)
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No information is available
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