|
Root transcripts associated with arsenic accumulation in hyperaccumulator Pteris vittata(Journal of Biosciences)
(Rasika M Potdukhe, Priyanka Bedi, Bijaya K Sarangi, Ram A Pandey and Sanjog T Thul
, 431,,105–115 ,Year : 2018)
|
Hyperaccumulation of arsenic (As) by brake fern Pteris vittata has been described as an important genetic trait that provides an option for development of a sustainable phytoremediation process for As mitigation. Accumulation of very high concentration of arsenic in above-ground tissues may be the result of arsenic vacuole compartmentalization, but the mechanism(s) of arsenic uptake and transport by underground tissues are largely unknown. In this study, we made an attempt towards understanding the molecular mechanism of As hyperaccumulation in this plant. A time-dependent As accumulation study indicates an exponential accumulation of As from 7 to 30 days of arsenic exposure in fronds, and day 3–7 in roots. Root transcriptome analysis identified 554,973 transcripts. Further, subsets of 824 transcripts were differ- entially expressed between treated and control samples. Many of the genes of critical As-stress response, transcription factors and metal transporters, biosynthesis of chelating compounds involved in uptake and accumulation mechanisms were identified. The genes that were highly expressed such as cysteine-rich RLK, and ABC transporter G family member 26 needs further studies along with arsenite transmembrane transporter. The analysis of generated transcriptome dataset has provided valuable information and platform for further functional studies.
|
|
|
Enhancement of indigo dye yield using fungal elicitors during fermentation of Indigofera plant biomass(Indian Journal of Experiment Biology)
(Saikat Dutta, El Mutaz Nassir Farag, Bijaya Ketan Sarangi
,,,,Year : 2018)
|
Natural indigo dye from biogenic materials is an alternative to synthetic indigo to alleviate the pollution scenario. Indigo production from the Indigofera tinctoria plant has been developed from ancient time begins through biomass fermentation in water. Maximum indigo yield depends on complete release of indigo precursor indican from plant biomass through fermentation. This study was carried out to assess the role of fungal elicitors on indigo dye yield during biomass fermentation using three strains namely; Aspergillus niger, Trichoderma reesei and Sclerotium rolfsii. Fungal extracts were prepared from mycelia raised separately on Potato Dextrose agar medium and extracted in a buffer solution containing 1N HNO3 and 1N NaOH. The extracts were added to fermentation water in 1%, 5% and 10% doses (v/v) separately during biomass fermentation keeping other parameters for fermentation and further processing for dye production fixed. Indigo yield with 10% A. niger extract was 1.56 % (w/w), which was higher in comparison to control 0.45% (w/w). A. niger extract at 10% enhanced indigo yield up to 3.5 times in comparison to other two cultures used at same concentration.
|
