|
Integrating Ultrasound with Activated Carbon prepared from Mangosteen Fruit Peel Waste Material for Effective Removal of Reactive Black 5 dye(Environmental Engineering and Management Journal)
(Randeep Singh, P.V. Nidheesh, T. Sivasankar,,,,Year : 2019)
|
|
No information is available
|
|
|
Molecular Mechanism of Apoptosis Induction in Jurkat E6-1 Cells by Tribulus Terrestris Alkaloids Extract(Traditional and Complementary Medicines)
(Shriniwas S. Basaiyye, Pravin K. Naoghare, Sanjeev Kanojiya, Amit Bafana, Patrizio Arrigo, Kannan Krishnamurthi, Saravanadevi Sivanesan,,,,Year : 2017)
|
The present study demonstrates apoptosis-inducing potential and mechanism of action of Tribulus terristris alkaloid extract in Jurkat E6-1 cancer cell line. Liquid Chromatography-Mass Spectrometry and High Resolution-Mass Spectrometry analysis identified the presence of four N-feruloyltyramine derivatives, namely trans-N-feruloyl-3-hydroxytyramine (1), trans-N-coumaroyltyramine (2), trans-N-feruloyltyramine (3) and trans-N-feruloyl-3-ethoxytyramine (4) in the alkaloid extract. Compounds 2 and 3 have not been yet reported in the alkaloid extract of T. terristris. In silico analysis revealed therapeutic potential of N-feruloyltyramine derivatives and strong binding efficiency to both chains of Tumor Necrosis Factor Receptor 1. Treatment of alkaloids extract to Jurkat E6-1 clone induced dose-dependent cytotoxicity (LC50 140.4 μg mL−1). Jurkat cells treated with alkaloids extract at sub-lethal concentration showed DNA fragmentation, enhancement in caspase-3 activity and phosphatidylserine translocation (apoptosis indicator) compared to control cells. Gene expression analysis using Human Apoptosis RT2 Profiler PCR Array analysis upon alkaloid treatment was found to significantly alter expression of critical genes such as TNFR1, FADD, AIFM, CASP8, TP53, DFFA and NFKB1. These genes are predicted to mediate apoptotic cell death via both intrinsic and extrinsic apoptosis pathway. In summary, we report the identification of new N-feruloyltyramine derivatives from alkaloid extract of T. terristris fruit with probable anti-leukemic and pharmacological potential.
|
|
|
Common Effluent Treatment Plants for Heterogeneous Cluster of Industries and Its Performance(National Journal of Life Sciences)
(Kishore Malviya and M. Suresh Kumar
, 11(2),,187-190,Year : 2014)
|
It has been almost over two decades since the concept of Common Ef?uent Treatment Plant (CETP), has been established successfully in India. CETPs have worked well for the cluster of similar type of Industries; however, the CETPs catering to a cluster of heterogeneous industries are still facing a problem of under performance resulting in regulatory issues. To address this problem, a CETP treating combined wastewater from heterogeneous cluster of 175 industries manufacturing different products, was studied. The CETP's performance and related problems were assessed by collecting wastewater samples from different stages of treatment and a detailed physico-chemical and bio-chemical analysis of these wastewater samples. The treat ability studies were conducted to address these problems, which were mostly pertaining to optimization of chemical additions in the primary treatment stages and addition of nutrients in the activated sludge process. The operational parameters were reset on the basis of outcome of the treatability studies and the performance of the CETP was again assessed after a period of 45 days of operation at reset operating conditions. The performance of the CETP substantially improved and the ?nal discharge could conform to the regulatory standards.
|
|
|
Solubilization and elimination of coliforms from sewage sludge by sonication(Journal of Environmental Science and Engineering (JESE))
(Snehal Pathki, M. Suresh Kumar and A.N. Vaidya.
,561: ,,89-92.,Year : 2014)
|
Disposal of sewage secondary excess sludge is a great problem globally, and stabilization of this excess sludge by anaerobic digestion is hampered due to its constituents resistant to biodegradation. Sludge pre-treatment enhances the performance of anaerobic digestion. In this study, sewage sludge was collected from a full-scale sewage treatment plant and characterized. Ultrasonic method was used for the excess sludge disintegration of microbial flocks and cells, so as to breakdown the intracellular or extracellular polymeric materials to enhance the anaerobic digestion. The studies related to the effect of sonication on release of nutrients, increase in soluble COD and reduction in pathogenic coliforms as well as heterotrophic microorganisms and the optimization of sonication time were carried out. The results showed that the twenty minutes sonication (25 kHz) increased the soluble COD content, nutrient release and complete disappearance of fecal as well as total coliforms in the treated sludge. The results are presented and discussed in this paper.
|
