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CSIR - NATIONAL ENVIRONMENTAL ENGINEERING RESEARCH INSTITUTE

सीएसआईआर-राष्ट्रीय पर्यावरण अभियांत्रिकी अनुसंधान संस्थान

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Green Skills Training Program on "Soft Quality Assessment and Land Management" on 16-17 September, 2025
Green Skills Training Program on
Green Skills Training Program on "Soft Quality Assessment and Land Management" on 16-17 September, 2025
CSIR-NEERI Delhi Zonal Centre organised a Stakeholders Consultation Meeting on 16 September 2025 for the up-gradation of 11 CETPs of Delhi.
CSIR-NEERI Delhi Zonal Centre organised a Stakeholders Consultation Meeting on 16 September 2025 for the up-gradation of 11 CETPs of Delhi. Image
CSIR-NEERI Delhi Zonal Centre organised a Stakeholders Consultation Meeting on 16 September 2025 for the up-gradation of 11 CETPs of Delhi.
CSIR-NEERI & CSIR-NIO Pavilion Shines at Empowering India - 2025 Expo, 12-14 Sept, Goa
CSIR-NEERI & CSIR-NIO Pavilion Shines at Empowering India - 2025 Expo, 12-14 Sept, Goa Image
CSIR-NEERI & CSIR-NIO Pavilion Shines at Empowering India - 2025 Expo, 12-14 Sept, Goa
Dr. Jitendra Singh,Hon'ble Minister of S&T and Vice President, CSIR leads CSIR's flood relief in J&K and CSIR-NEERI team provided NEERI-ZAR drinking water systems to affected communities
Dr. Jitendra Singh,Hon'ble Minister of S&T and Vice President, CSIR leads CSIR's flood relief in J&K and CSIR-NEERI team provided NEERI-ZAR drinking water systems to affected communities Image
Dr. Jitendra Singh,Hon'ble Minister of S&T and Vice President, CSIR leads CSIR's flood relief in J&K and CSIR-NEERI team provided NEERI-ZAR drinking water systems to affected communities
Students of B.Tech AI & Data Science, Wainganga College, explore CSIR-NEERI for environmental solutions
Students of B.Tech AI & Data Science, Wainganga College, explore CSIR-NEERI for environmental solutions Image
Students of B.Tech AI & Data Science, Wainganga College, explore CSIR-NEERI for environmental solutions
CSIR-NEERI, Nagpur and the State Institute of Health and Family Welfare, Nagpur, signed a Memorandum of Understanding (MoU) on 4th September 2025
CSIR-NEERI, Nagpur and the State Institute of Health and Family Welfare, Nagpur, signed a Memorandum of Understanding (MoU) on 4th September 2025 Image
CSIR-NEERI, Nagpur and the State Institute of Health and Family Welfare, Nagpur, signed a Memorandum of Understanding (MoU) on 4th September 2025
Concluding Session of Capacity Building Training Program
Concluding Session of Capacity Building Training Program Image
Concluding Session of Capacity Building Training Program
Rear Admiral Sandeep Mehta, Director General, Project Varsha, Ministry of Defence, visited the CSIR-NEERI Hyderabad Zonal Centre (HZC) on 28 August 2025
Rear Admiral Sandeep Mehta, Director General, Project Varsha, Ministry of Defence, visited the CSIR-NEERI Hyderabad Zonal Centre (HZC) on 28 August 2025 Image
Rear Admiral Sandeep Mehta, Director General, Project Varsha, Ministry of Defence, visited the CSIR-NEERI Hyderabad Zonal Centre (HZC) on 28 August 2025
Dr Lal Singh, Principal Scientist at CSIR-NEERI, has been honored as one of five "Sustainability Changemakers"
Dr Lal Singh, Principal Scientist at CSIR-NEERI, has been honored as one of five
Dr Lal Singh, Principal Scientist at CSIR-NEERI, has been honored as one of five "Sustainability Changemakers"
Dr. S. Venkata Mohan, Director CSIR-NEERI, delivered a talk on "Surveillance and Management of Antibiotic Resistance in Wastewater"
Dr. S. Venkata Mohan, Director CSIR-NEERI, delivered a talk on
Dr. S. Venkata Mohan, Director CSIR-NEERI, delivered a talk on "Surveillance and Management of Antibiotic Resistance in Wastewater"
Dr. Noor Afshan Khan and Dr. Ankit Gupta, Principal Scientists from the CSIR-NEERI DZC, recently undertook a deputation visit to Germany and the United States
Dr. Noor Afshan Khan and Dr. Ankit Gupta, Principal Scientists from the CSIR-NEERI DZC, recently undertook a deputation visit to Germany and the United States Image
Dr. Noor Afshan Khan and Dr. Ankit Gupta, Principal Scientists from the CSIR-NEERI DZC, recently undertook a deputation visit to Germany and the United States
CSIR-NEERI celebrated Independence Day with great enthusiasm.
CSIR-NEERI celebrated Independence Day with great enthusiasm. Image
CSIR-NEERI celebrated Independence Day with great enthusiasm.
Officials from M/s Cochin Minerals and Rutile Limited (CMRL), visited CSIR-NEERI
Officials from M/s Cochin Minerals and Rutile Limited (CMRL), visited CSIR-NEERI Image
Officials from M/s Cochin Minerals and Rutile Limited (CMRL), visited CSIR-NEERI
Archive
Archive Publications

Archive Publications

An insight into phage diversity at environmental habitats using comparative metagenomics approach (Current Microbiology)
(Krupa M. Parmar, Rajesh R. Pal, Nishant A. Dafale, Hemant J. Purohit,75,,32-141 , Year: 2018)

Bacteriophages play significant role in driving microbial diversity; however, little is known about the diversity of phages in different ecosystems. A dynamic predator–prey mechanism called ‘‘kill the winner’’ suggests the elimination of most active bacterial populations through phages. Thus, interaction between phage and host has an effect on the composition of microbial communities in ecosystems. In this study, secondary phage metagenome data from aquatic habitats: wastewater treatment plant (WWTP), fresh, marine, and hot water spring habitat were analyzed using MG-RAST and STAMP tools to explore the diversity of the viruses. Differential relative abundance of phage families—Siphoviridae (34%) and Myoviridae (26%) in WWTP, Myoviridae (30%) and Podoviridae (23%) in fresh water, and Myoviridae (41%) and Podoviridae (8%) in marine—was found to be a discriminating factor among four habitats while Rudiviridae (9%), Globuloviridae (8%), and Lipothrixviridae (1%) were exclusively observed in hot water spring. Subsequently, at genera level, Bpp-1-like virus, Chlorovirus, and T4-like virus were found abundant in WWTP, fresh, and marine habitat, respectively. PCA analysis revealed completely disparate composition of phage in hot water spring from other three ecosystems. Similar analysis of relative abundance of functional features corroborated observations from taxa analysis. Functional features corresponding to phage packaging machinery, replication, integration and excision, and gene transfer discriminated among four habitats. The comparative metagenomics approach exhibited genetically distinct phage communities among four habitats. Results revealed that selective distribution of phage communities would help in understanding the role of phages in food chains, nutrient cycling, and microbial ecology. Study of specific phages would also help in controlling environmental pathogens including MDR bacterial populations using phage therapy approach by selective mining and isolation of phages against specific pathogens persisting in a given environment.

In situ ammonia removal by methanogenic granular biomass
(Shaswati Saha, Jo De Vrieze, Rima Biswas Mondal, Tapas Nandy,Volume 4,,page 559-568, Year: 2018)
No information is available
Carbon Nanoparticles for Solar Disinfection of Water (Journal Hazardous Materials)
(Pratap Reddy Maddigpu, Bhairavi Sawant, Snehal Wanjari, M. D. Goel, Davide Vione, Rita S. Dhodapkar, S. Rayalu,volume 343,,page 157-165, Year: 2018)
No information is available
An overview on the removal of synthetic dyes from water by electrochemical advanced oxidation processes (Chemosphere)
(P.V. Nidheesh, M. Zhou, M. A. Oturan ,197,, 210-227, Year: 2018)

Wastewater containing dyes are one of the major threats to our environment. Conventional methods are insuf?cient for the removal of these persistent organic pollutants. Recently much attention has been received for the oxidative removal of various organic pollutants by electrochemically generated hydroxyl radical. This review article aims to provide the recent trends in the ?eld of various Electrochemical Advanced Oxidation Processes (EAOPs) used for removing dyes from water medium. The characteristics, fundamentals and recent advances in each processes namely anodic oxidation, electro-Fenton, peroxicoagulation, fered Fenton, anodic Fenton, photoelectro-Fenton, sonoelectro-Fenton, bioelectro-Fenton etc. have been examined in detail. These processes have great potential to destroy persistent organic pollutants in aqueous medium and most of the studies reported complete removal of dyes from water. The great capacity of these processes indicates that EAOPs constitute a promising technology for the treatment of the dye contaminated ef?uents.

Review of zero-valent aluminium based water and wastewater treatment methods (Chemosphere)
(P. V. Nidheesh, Jayraj Khatri, T. S. Anantha Singh, R. Gandhimathi, S. T. Ramesh ,200,, 621-631, Year: 2018)

Zero-valent metals (ZVM) are widely used to remove heavy metals, contaminants, toxicity, etc. from water and wastewater. Zero-valent aluminium (ZVAl) has large surface area and high surface reactivity. It has enormous ?exibility for the in-situ application. ZVAl can be applied as either a single or a bimetallic system as well as advanced oxidation processes (AOPs). It is observed that ZVAl is capable of generating hydroxyl and sulfate radicals in water medium, which remove non-biodegradable pollutants from aqueous solution. ZVAl-based processes can remove non-biodegradable organic contaminants from water mediumwithin a short duration. ZVAl is also used as a reducing agent. It is eficient to reduce toxic hexavalent chromium to less toxic trivalent chromium. ZVAl, in various combinations in bimetallic system (Fe/Al, Pd/Al, Cu/Al), is able to remove various contaminants from aqueous medium. Overall, it can be concluded that ZVAl-based methods for water and wastewater treatment are promising environmental technologies.

Gut-Bioreactor and Human Health in Future (Indian Journal of Microbiology)
(Purohit, H. J. ,58(1) ,,3-7, Year: 2018)
No information is available
Removal of Rhodamine B dye from aqueous solution by electro-Fenton process using iron-doped mesoporous silica as a heterogeneous catalyst (Chemosphere)
(R. Jinisha, R. Gandhimathi, S. T. Ramesh, P. V. Nidheesh, S. Velmathi ,200,,446-454, Year: 2018)

In the current study, Rhodamine B (RhB) dye was removed by electro-Fenton (EF) process using iron-doped SBA-15 (Fe-SBA-15; SBA: Santa Barbara Amorphous) mesoporous silica as a heterogeneous catalyst. This catalyst was prepared with the help of ferric nitrate nonahydrate as a forerunner by wet impregnation method. Various techniques of characterization such as FTIR studies, XRD, SEM and N2 adsorption-desorption isotherms were performed to confirm the presence of iron particles in the pores of the catalyst. These characterization methods were also used to examine the morphological properties and textural arrangement of the synthesized material. In the batch study of EF process, 750 mL working volume of RhB dye was taken. Anode and cathode used in the process were graphite electrodes respectively with effective area of 25 cm2 each. To maximise the process efficiency, the effect of initial pH, applied voltage, electrode spacing, the concentration of supporting electrolyte and Fe-SBA-15 dosage were investigated and optimized. The optimum conditions obtained were pH of 2, voltage of 8 V, an electrode spacing of 3 cm and Fe-SBA-15 dosage of 15 mg L-1 . At the end of 3 h electrolysis, maximum RhB removal of 97.7% and TOC removal of 35.1% were achieved for 10 mg L-1 RhB concentration. In a batch study with real wastewater, 97% of color and 39% of TOC were removed at optimum conditions. Utilization of EF heterogeneous catalyst Fe-SBA-15 is an alternative technique for the elimination of dyes from solution.

Annotation and De Novo Sequence Characterization of Extracellular B- Fructofranosidase from Penicillium chrysogenum Strain HKF42 (Indian Journal of Microbiology)
("Gujar, N.; Fuke, P.; Khardenavis, A. A.; Purohit , H. J. " ,58(2),,227- 233, Year: 2018)
No information is available
Composite Wastewater Treatment by Aerated Electrocoagulation and Modified Peroxi-coagulation Processes (Chemosphere)
(Abhijeet Kumar, P. V. Nidheesh, M Suresh Kumar ,,, 587-593, Year: 2018)

Treatment of composite wastewater generating from the industrial estates is a great challenge. The present study examines the applicability of aerated electrocoagulation and modified peroxi-coagulation processes for removing color and COD from composite wastewater. Iron plates were used as anodes and cathodes in both electrochemical processes and experiments were carried out in a working volume of 2 L. Aeration enhanced the efficiency of electrocoagulation process significantly. More than 50% of COD and 60% of color were removed after 1 h of electrocoagulation process operated at pH 3 and applied voltage of 1 V. Eficiency of the modified peroxi-coagulation process was significantly higher than that of aerated electrocoagulation. COD and color removal efficiencies of the modified peroxi-coagulation process were found as 77.7% and 97%, respectively after 1 h of electrolysis operated at 1 V, solution pH 3 and 50 mM hydrogen peroxide addition. This improved eficiency of modified peroxi-coagulation compared to aerated electrocoagulation is mainly due to the attack of in-situ generated hydroxyl radicals.

Advanced oxidation processes based on zero-valent aluminium for treating textile wastewater (Chemical Engineering Journal)
(Jayraj Khatri, P.V. Nidheesh, T.S. Anantha Singh, M. Suresh Kumar,348,,67-73, Year: 2018)

Treatment of textile wastewater by advanced oxidation processes (AOPs) based on zero-valent aluminium (ZVAl) were carried out in the present study. COD removal efficiencies of ZVAl/O2, ZVAl/Fe3+/O2, ZVAl/Fe3+/O2/H2O2 and ZVAl/Fe3+ /O2/persulfate processes were monitored and optimized. Colour and ammoniacal nitrogen removal efficiencies of each process at the optimal operating conditions were compared. Pollutant removal efficiencies of AOPs were followed the order as: ZVAl/Fe3+/O2/H2O2> ZVAl/Fe3+/O2/persulfate > ZVAl/Fe3+/O2> ZVAl/O2. Maximum COD, colour and ammoniacal nitrogen removal e?ciencies of ZVAl-based AOPs were found as 97.9%, 94.4% and 58.3%, respectively at 1 g/L ZVAl, 0.5 g/L Fe3+, 6.7 g/L H2O2 and after 3 h of contact time. External addition of tert-butyl alcohol to the processes revealed that in-situ hydroxyl and sulfate radicals are the main oxidants responsible for the oxidation of pollutants. Overall, ZVAl-based AOPs are efficient for treating effuents generating from textile industry.

Iron impregnated biochars as heterogeneous Fenton catalyst for the degradation of acid red 1 dye (Journal of Environmental Management)
(K.K. Rubeena, P. Hari Prasad Reddy, A.R. Laiju, P.V. Nidheesh,226,,320-328, Year: 2018)

In the present work, Acid Red 1 (AR1) dye degradation by two heterogeneous Fenton catalysts, namely iron loaded rice husk biochar (Fe-RHB) and coir pith biochar (Fe-CPB) are studied. Biochar prepared from RHB and CPB were sonicated in the presence of ferric nitrate for the synthesis of Fe-RHB and Fe-CPB by incipient impregnation ethod. E?ect of operational parameters such as pH, the dosage of catalyst, H2O2 concentration and temperature were examined. Characterization of the synthesized Fenton catalyst, Fe-RHB and Fe-CPB were analysed by SEM, EDS, XRD and XPS techniques. In Fe-RHB Fenton system, maximum dye removal e?ciency of 97.6% and TOC removal e?ciency of 84.2% were obtained at pH 3 for 50 mg L-1 of AR1 concentration, with 16 mM of H2O2 and 5 g L-1 of catalyst dosage within 120 min reaction time. Similarly, for Fe-CPB, maximum dye removal e?ciency of 99.1% and TOC removal e?ciency of 86.7% were obtained with 16 mM of H2O2 and 4gL-1 of dosage for 50 mg L-1 of initial dye concentration at pH 3. The prepared catalysts can be reused for successive cycles as the catalyst materials are highly stable and have very less iron leaching property.

Removal of organic pollutants by peroxicoagulation (Environmental Chemistry)
( P.V. Nidheesh ,,,, Year: 2018)

Peroxicoagulation is an electrochemical advanced oxidation processes in which both ferrous ions and hydrogen peroxide are generated in the cell.Organic pollutants are thus removed by degradation and coagulation. The peroxicoagulation process is a combination of electroFenton and electrocoagulation processes. The peroxicoagulation process is very eicient_for the  removal of aniline and herbicides from water and for the treatment of landfll leachate and textile wastewaters. Under acidic conditions, electro- Fenton is the predominant removal means, whereas electrocoagulation is the main removal means under neutral and alkaline conditions. As a consequence, pH regulation toacidic conditions is essential for the mineralization of organic pollutants.

Root transcripts associated with arsenic accumulation in hyperaccumulator Pteris vittata (Journal of Biosciences)
(Paotdukhe, R. M.; Bedi, P.; Sarangi, B. K. ; Pandey, R. A.; Purohit, H. J. ,431,,105- 115, Year: 2018)
No information is available
Catalytic ozonation for the treatment of synthetic and industrial effluents - Application of mesoporous materials: A review (Journal of Environmental Management)
(Santosh P. Ghuge, Anil K. Saroha ,211,,83-102, Year: 2018)
Several industries generate a large amount of effluent containing concentrated refractory organic compounds. Numerous conventional techniques are available for the treatment of the effluent but these techniques have some limitations like less degradation efficiency, more power consumption and unsuitable for high toxicity level. Catalytic ozonation has been receiving great attention in recent years to tackle the limitations of conventional effluent treatment processes. Various materials have been explored as catalysts in the catalytic ozonation to enhance degradation of organic compounds present in the effluent. The present paper includes superficial review of catalytic ozonation studies performed using different catalysts like alumina, activated carbon, ceria etc. The mesoporous materials have unique surface properties such as large surface area, uniform pore sizes and thick pore walls. These surface properties play vital role in facilitating mineralization of refractory organic compounds. The present work aims to review the information available on the use of mesoporous materials in catalytic ozonation. It was observed that mesoporous materials are utilized in only 15 % of the total catalytic ozonation studies published in the literature during the last 12 years (2005-17). The catalytic ozonation studies performed using mesoporous materials have been tabulated in the present work. It has also been observed from the overall review that around 90 % of catalytic ozonation studies are performed for the treatment of synthetic effluent rather than industrial effluent. It is, therefore, needed to study the application of catalytic ozonation for the treatment of industrial effluent. It has been found that nearly half of the reported catalytic ozonation studies for the treatment of industrial effluent are of specific types of industries like textile and petroleum refineries. The catalytic ozonation studies for the treatment of industrial effluent have also been listed in this manuscript. It would be interesting to apply catalytic ozonation involving mesoporous materials for the treatment of effluent of different industries to study its efficiency, applicability and influence of different operational parameters. The authors offer suggestions of treating industrial effluent by catalytic ozonation using mesoporous materials due to their unique textural properties.
Catalytic ozonation of dye industry effluent using mesoporous bimetallic Ru-Cu/SBA-15 catalyst (Process Safety and Environmental Protection)
(Santosh P. Ghuge, Anil K. Saroha ,118,,125-132., Year: 2018)

Catalytic ozonation was employed for the degradation of aqueous solution containing reactive orange4 (RO4) azo dye and textile dye industry effluent using mesoporous bimetallic Ru-Cu/SBA-15 catalyst. The catalyst was synthesized and characterized using BET, SEM, TEM and EDX techniques. The effect of operational parameters like initial solution pH and ozone dose on the degradation of RO4 azo dye was studied. The degradation pathway of the dye solution was investigated using radical scavenger t-butanol. The optimum values of initial solution pH and ozone dose were found to be 9 and 5 g/m3 respectively. A colour removal efficiency of 100% and COD removal efficiency of 70.4% of RO4 dye aqueous solution were obtained at the optimum conditions after 21 min and 60 min of catalytic ozonation respectively. The colour and COD removal pathways were governed by direct ozone molecular and hydroxyl radical mechanism respectively. The bicarbonate ions present in the textile dye industry effluent were found to act as radical scavengers, thereby, lowering the COD removal efficiency significantly. The removal of bicarbonate ions from the effluent prior to its ozonation enhanced the COD removal efficiency from 30.2% to 90% after 4 h of catalytic ozonation.

Ozonation of Reactive Orange 4 dye aqueous solution using mesoporous Cu/SBA-15 catalytic material (Journal of Water Process Engineering)
(Santosh P. Ghuge, Anil K. Saroha ,23,,217-229., Year: 2018)

The catalytic ozonation using mesoporous SBA-15 supported copper catalyst (Cu/SBA-15) was employed for the degradation of reactive orange 4 (RO4) azo dye in aqueous solution. The catalyst was synthesized and characterized using BET, SEM, TEM and EDX techniques. The characteristic results revealed highly ordered mesoporous structure, uniform pore sizes, parallel arranged channels and large surface area for the catalyst. The effects of copper loading on SBA-15, initial solution pH and ozone dose on the degradation of RO4 dye were studied. The complete colour removal from RO4 azo dye aqueous solution was obtained after 21 min of ozonation. The maximum TOC removal efficiency of 86% was obtained at optimum operational conditions of 2% copper loadings, initial solution pH 9 and ozone dose of 5 g/m3 after 60 min reaction time. The degradation pathway of catalytic ozonation reaction was studied by adding t-butanol as radical scavenger and it was found that the presence of radical scavenger had no effect on colour removal efficiency suggesting direct ozone molecular type mechanism. However, TOC removal efficiency was significantly reduced with the addition of t-butanol, thereby, indicating a hydroxyl radical based mechanism. The catalytic ozonation reaction followed pseudo-first order kinetics with respect to RO4 dye concentration. The reusability study for the catalyst suggested a negligible loss in the degradation efficiency after five runs of experiments. The stability of the catalyst was studied by performing leaching experiments which suggested that the catalyst has the potential to be utilized for the degradation of aqueous dye solution.

Ozonation of Reactive Orange 4 dye aqueous solution using mesoporous Cu/SBA-15 catalytic material (Safety Science)
(Ravi K. Sharma, Bhola R. Gurjar, Akshay V. Singhal, Satish R. Wate, Santosh P. Ghuge, Rajat Agrawal,72,,262-273., Year: 2018)

In the recent decades, several fatal accidents have occurred around the world at petroleum storage sites leading to fire, explosion and toxic release scenarios. Such accidents result in huge loss of lives and property, along with widespread environmental damage due to improper coordination and communication in the emergency response. Therefore, emergency response planning is an integral and essential part of the safety and loss prevention strategy and comprises of the actions taken to manage, control and mitigate the immediate effects of an incident. An emergency requires a crystal clear hierarchy of command and organizational-procedural guidelines without any ambiguities. This paper highlights the need, structure and development of an automated networking system, called the electronic-Incident Command System (e-ICS), through case study of the Indian Oil Corporation Limited (IOCL) Jaipur storage terminal accident. Based on the previously proposed Incident Command System, e-ICS further strengthens coordination and communication in emergency responses. The response mechanism, through Confirmation, Command, Tactical and Support Nets for vertical and horizontal flow of commands and information has been described. This paper also discusses the importance of the Emergency Operations Center and Emergency Management Computation System in expediting the information flow. Thus, recommendations for improving coordination in crisis management are proposed.

Cross-Reactivity of Prokaryotic 16S rDNA-Specific Primers to Eukaryotic DNA: Mistaken Microbial Community Profiling in Environmental Samples (Current Microbiology)
(Yadav, S.; Kumar, A.; Gupta, M.; Maitra, S. S.,,,1-8, Year: 2018)
No information is available
An Approach to In Silico Dissection of Bacterial Intelligence Through Selective Genomic Tools (Indian Journal of Microbiology)
(Talkal, R.; Tikariha, H.; Purohit, H. ,,,1-9, Year: 2018)
No information is available
Passive Solar still: Recent advancement in design and related Performance (Environment Technology)
(Kanchan Kumari ,31,,1-59. , Year: 2018)

Present review paper mainly focuses on different varieties of solar stills and highlights mostly the passive solar still with advanced modifications in the design and development of material, single and multi-effect solar still with augmentation of different materials, energy absorbing, insulators, mechanisms of heat and mass transfer to improve the loss of heat and enhance the productivity of solar still. The cost-benefit analysis along with the progressive advancement for solar stills is the major highlights of this review. To increase the output of solar still nowadays, applications of advance modifications is one of the promising tools, and it is anticipated that shortly more vigor will be added in this area with the modifications in designs of solar stills.

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