|
Integrated bio-oxidation and adsorptive filtration reactor for removal of arsenic from wastewater(Environmental Technology (United Kingdom))
(Kamde, K;
Dahake, R;
Pandey, R. A;
Bansiwal, A.
,,,,Year : 2016)
|
|
No information is available
|
|
Resultados de un programa integrado de mitigacn de fluorisis en una poblacin controlada de India(Journal of Hydraulics)
(Godfrey Sam, Labhasetwar Pawan, Dutta Samir, Vol 1,Number 1,p 35-45,Year : 2010)
|
|
No information is available
|
|
|
DNA mini-barcoding: an approach for forensic identification of some endangered Indian snake species(Forensic Science International: Genetics)
(Dubey, B., Meganathan, P. R., Haque. I
,,,181-184,Year : 2011)
|
|
Illegal trade of snake skin and uncontrolled hunting have instigated the extermination of many endangered snake species. Efforts to check illegal trade are often impeded due to lack of proper species identification methods. Hence, conservation strategies demand for authentic and quick identification techniques to trace the origin of the seized samples. This study employs DNA mini-barcoding as a method to identify some endangered snake species of India. We have designed two sets of novel primers for targeting regions within the mitochondrial Cytochrome Oxidase I gene to produce 175 bp and 245 bp amplicons. 175 bp fragment was amplified in all 11 snake species studied while the 245 bp amplicon was obtained in 10 species. DNA mini-barcodes recovered from these amplicons enabled the identification of snake species by retrieving the sequences available in public databases. The similarity scores ranging from 98 to 100% (98% taken as threshold value for species identification) signify the consistency of these mini-barcodes in snake species identification. Moreover, the results of the validation study confirm the effectiveness of the technique in forensic perspective, where the diagnostic morphological features of the seized sample are often missing.
|
|
|
Moringa Oliefera: The Miracle Tree(International Journal of Advance Research Ideas and Innovation in Technology)
(Ruchita Haldar and Sharda Kosankar ,3(6),,966-970,Year : 2018)
|
|
No information is available
|
|
|
Safe greywater reuse to augment water supply and provide sanitation in semi arid areas of rural India, Paper accepted for publication in Water (Environmental Science and Technology)
(Godfrey S, Labhasetwar P, Wate S, Jimenez B,,,,Year : 2010)
|
|
No information is available
|
|
|
Molecular phylogenetic analyses of Genus Crocodylus (Eusuchia, Crocodylia, Crocodylidae) and the taxonomic position of Crocodylus porosus.(Molecular Phylogenetics and Evolution,)
(Meganathan, P. R., Dubey, B., Batzer, M. A., Ray, D. A., Haque.I
,,,393-402,Year : 2010)
|
|
The genus Crocodylus consists of 11 species including the largest living reptile, Crocodylus porosus. The current understanding of the intrageneric relationships between the members of the genus Crocodylus is sparse. Even though members of this genus have been included in many phylogenetic analyses, different molecular approaches have resulted in incongruent trees leaving the phylogenetic relationships among the members of Crocodylus unresolved inclusive of the placement of C. porosus. In this study, the complete mitochondrial genome sequences along with the partial mitochondrial gene sequences and a nuclear gene, C-mos were utilized to infer the intrageneric relationships among Crocodylus species with a special emphasis on the phylogenetic position of C. porosus. Four different phylogenetic methods, Neighbour Joining, Maximum Parsimony, Maximum Likelihood and Bayesian inference, were utilized to reconstruct the crocodilian phylogeny. The uncorrected pairwise distances computed in the study, show close proximity of C. porosus to C. siamensis and the tree topologies thus obtained, also consistently substantiated this relationship with a high statistical support. In addition, the relationship between C. acutus and C. intermedius was retained in all the analyses. The results of the current phylogenetic study support the well established intergeneric crocodilian phylogenetic relationships. Thus, this study proposes the sister relationship between C. porosus and C. siamensis and also suggests the close relationship of C. acutus to C. intermedius within the genus Crocodylus.
|
|
|
Infestation of Pauropsila tuberculata (Gall Insect) on Alstonia scholaris (Saptaparni) tree
(Ruchita Haldar, Sharda Kosankar
,6(1),,1695-1699,Year : 2018)
|
|
No information is available
|
|
|
Status, distribution and correlation of arsenic in groundwater and its health effects to the inhabitants of Gaighata block, West Bengal, India(Chemosphere)
(T. Roychowdhury,,,,Year : 2010)
|
|
No information is available
|
|
|
A novel multiplex PCR assay for the identification of Indian crocodiles. (Molecular Ecology Resources)
(Meganathan, P. R., Dubey B., Jogayya, K. N., Whitaker, N., Haque. I
,,,744- 747,Year : 2010)
|
|
Illegal hunting has been a major threat for the survival of wildlife fauna, including the three crocodile species that India harbours: Crocodylus palustris, Crocodylus porosus and Gavialis gangeticus. Although law prevents trade on these species, illicit hunting for trade continues to threaten the survival of these endangered species; conservation strategies therefore require a rapid molecular identification technique for Indian crocodiles. A multiplex polymerase chain reaction (PCR) assay with species-specific primers, considered as one of the most effective molecular techniques, is described herein. The primers were designed to yield species-specific sized amplicons. The assay discriminates the three Indian crocodile species unambiguously within a short time period using only simple agarose gel electrophoresis. We recommend this multiplex PCR assay to be used in the identification of Indian crocodile species.
|
|
|
Faunal diversity of CSIR-NEERI campus, Nagpur, Maharashtra, India(Int J. of Life Sciences )
(Kiran Bawaskar, Ruchita Haldar, Sharda Kosankar
,62,,311-325,Year : 2018)
|
|
No information is available
|
|
|
Groundwater arsenic contamination in one of the 107 arsenic affected blocks in West Bengal, India
(,,,,Year : 2017)
|
|
No information is available
|
|
|
Molecular identification of three Indian snake species using simple PCR-RFLP method(Journal of Forensic Sciences)
(Dubey, B., Meganathan, P. R., Haque. I
,,,1065-1067,Year : 2010)
|
|
Three endangered Indian snake species, Python molurus, Naja naja, and Xenochrophis piscator are known to be significantly involved in illegal trade. Effective authentication of species is required to curb this illegal trade. In the absence of morphological features, molecular identification techniques hold promise to address the issue of species identification. We present an effective PCR-restriction fragment length polymorphism method for easy identification of the three endangered snake species, Python molurus, Naja naja, and Xenochrophis piscator. A 431-bp amplicon from cytochrome b gene was amplified using novel snake-specific primers following restriction digestion with enzymes Mbo II and Fok I. The species-specific reference fragment patterns were obtained for the target species, which enabled successful identification of even highly degraded shed skin sample confirming the utility of the technique in case of poor-quality DNA. The assay could be effectively used for forensic authentication of three Indian snake species and would help strengthen conservation efforts.
|
|
|
Microbially assisted arsenic removal using Acidothiobacillus ferrooxidans mediated by iron oxidation(Environmental Technology & Innovation)
(K. Kamde, R.A.Pandey, S.T. Thul, R. Dahake, V. M. Shinde, A. Bansiwal
,10,,78–90,Year : 2018)
|
The aim of this investigation was to determine the efficiency of arsenic removal present in acidic water using the biochemical method in presence of Acidothiobacillus ferrooxidans bacteria and ferrous ions. The process includes the bio-oxidation of ferrous to ferric by bacteria with simultaneous conversion of arsenite to arsenate and its removal by chemical method. The experiments were carried out in shake flasks with or without A.ferrooxidans culture. The variables of the pH, temperature, cell density and initial concentration of ferrous ions in the growth medium of A.ferrooxidans bacteria were examined in the laboratory to determine how they affect the removal of arsenic. It was found that the maximum arsenic removal (80%–85%) occurred at ferrous ions concentration of 2 gL−1 at the temperature of 35 ?C with the initial cell density of 3.66 × 107 cells/mL and initial pH value of 2.5 with 70%–75% bio-oxidation of ferrous ions. The results of scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses have confirmed the growth of bacteria and identify the nature of precipitate generated by the bacteria. The finding showed that arsenic can be removed considerably in this process in an efficient and greener way, but also removing efficiency was much more (<80%) in acidic wastewater. The ANOVA and LSD statistical analysis were performed to assess any significant differences occurred among the four different levels of each variable. This study verified that combination of bio-oxidation with filtration improves the removal efficiency of arsenic.
|
|
|
How safe are the global water coverage figures? Case study from Madhya Pradesh, India(Environment Monitoring and Assessment)
(Sam Godfrey, Pawan Labhasetwar, Satish Wate and Sarika Pimpalkar,,,,Year : 2010)
|
|
No information is available
|
|
|
Traces of sub-Saharan and Middle Eastern lineages in Indian Muslim populations. (European Journal of Human Genetics)
(Eaaswarkhanth, M., Haque, I., Ravesh, Z., Romero, I. G., Meganathan P. R., Dubey, B., Khan, F. A., Chaubey, G., Kivisild, T., Tyler-Smith, C., Singh, L., Thangaraj, K
,,,354- 363,Year : 2010)
|
Islam is the second most practiced religion in India, next to Hinduism. It is still unclear whether the spread of Islam in India has been only a cultural transformation or is associated with detectable levels of gene flow. To estimate the contribution of West Asian and Arabian admixture to Indian Muslims, we assessed genetic variation in mtDNA, Y-chromosomal and LCT/MCM6 markers in 472, 431 and 476 samples, respectively, representing six Muslim communities from different geographical regions of India. We found that most of the Indian Muslim populations received their major genetic input from geographically close non-Muslim populations. However, low levels of likely sub-Saharan African, Arabian and West Asian admixture were also observed among Indian Muslims in the form of L0a2a2 mtDNA and E1b1b1a and J(*)(xJ2) Y-chromosomal lineages. The distinction between Iranian and Arabian sources was difficult to make with mtDNA and the Y chromosome, as the estimates were highly correlated because of similar gene pool compositions in the sources. In contrast, the LCT/MCM6 locus, which shows a clear distinction between the two sources, enabled us to rule out significant gene flow from Arabia. Overall, our results support a model according to which the spread of Islam in India was predominantly cultural conversion associated with minor but still detectable levels of gene flow from outside, primarily from Iran and Central Asia, rather than directly from the Arabian Peninsula.
|
|
|
Drinking water quality monitoring and surveillance for safe water supply in Gangtok, India(Environment Monitoring and Assessment)
(Gajanan K. Khadse, Morami Kalita, Sarika N. Pimpalkar and Pawan K. Labhasetwar,,,,Year : 2010)
|
|
No information is available
|
|
|
Molecular identification of Indian crocodile species: PCR-RFLP method for forensic authentication(Journal of Forensic Sciences)
(Meganathan, P. R., Dubey, B., Haque. I
,,,1042- 1045,Year : 2009)
|
|
South East Asian countries are known for illegal poaching and trade of crocodiles clandestinely, to be used in skin, medicinal, and cosmetic industries. Besides crocodiles being listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora, India has its Wildlife Protection Act, 1972 for conservation of crocodile species. Hitherto, lack of any rapid and reliable technique for examinations of crocodile-based crime exhibits such as skin, bones, etc. has been a major problem for an effective promulgation of law on illegal trade. DNA-based identification of species using PCR-RFLP technique for an apt identification of all the three Indian crocodile species namely, Crocodylus porosus, Crocodylus palustris and Gavialis gangeticus is presented here. A 628 bp segment of cytochrome b gene was amplified using novel primers followed by restriction digestion with three enzymes i.e., HaeIII, MboI, and MwoI, separately and in combination. The technique has produced a species-specific pattern for identifying the three crocodile species individually, which fulfills the requirement for its forensic application. It is expected that the technique will prove handy in identification of all the three Indian crocodile species and strengthen conservation efforts.
|
|
An Integrated Process of Value Addition to Citrus Waste and Performance of Fenton Process for Its Conversion to Biogas(Waste and Biomass Valorization)
(M. E. Magare, N. Sahu, G. S. Kanade, C. S. Chanotiya, S. T. Thul
,,,,Year : 2018)
|
Citrus fruits are commercially important fruit crop all over the world. About 50–60% of citrus fruit is considered waste including peels and its handling is costly at municipal and industry levels. Citrus wastes in general show a low pH (3–4), relatively high water content and organic matter. Its composting is not recommended, as it has inherent low pH and presence of high concentration of limonene in essential oils that slow down its biological decomposition due to inhibitory activity. Anaerobic digestion for biogas production seems to be a technically sustainable way to valorise by suitable pre-treatment methods. Present study shows extraction of essential oil in minimum requirement of water (1:1 w/v) for hydro-distillation that yields about 2% essential oil from fresh peels. Further, pre-treatment of left over de-oiled biomass with of 30% Fenton’s reagent treatment show biogas and methane production up to 322.63 ml biogas/g VS feed and 122.48 ml methane/g VS feed, respectively under mesophilic condition which is superior to conventional treatment.
|
|
|
Multiplex PCR assay for rapid identification of three endangered snake species of India. (Conservation Genetics)
(Dubey, B., Meganathan, P. R., Haque.I
,,,1861- 1864,Year : 2009)
|
|
Species identification has been the core issue in all approaches of conservation of endangered wild life. In this regard molecular techniques for species authentication have proved indispensable. A novel multiplex PCR assay for the identification of three Indian snake species Python morulus, Ptyas mucosus, and Naja naja is successfully demonstrated using 16S rRNA gene. Three reverse primers and a common forward primer were designed to generate three different size species-specific PCR fragments. Absence of any PCR amplification in non-target species proves the specificity of the primers. These four primers were combined in a multiplex assay to enable identification of three snake species in a single reaction. The assay described here shows its utility in identifying unknown snake specimen and in case of samples yielding low quality DNA. This multiplex PCR technique using novel primers is an unprecedented approach offered for forensic identification of exhibits originating from three Indian snake species. It is expected that this endeavor will help strengthening conservation efforts for these species.
|
|
|
Removal of arsenic by Acidothiobacillus ferrooxidans bacteria in bench scale fixed-bed bioreactor system(Chemistry and Ecology)
(K. Kamde, R.A. Pandey, S. Thul, A. Bansiwal,,,,Year : 2018)
|
In the present study arsenic contaminated simulated water and groundwater was treated by the combination of biological oxidation of tri-valent arsenite [As (III)] to penta-valent arsenate [As (V)] in presence of Acidothiobacillus ferrooxidans bacteria and its removal by adsorptive filtration in a bioreactor system. This method includes the immobilisation of A.ferrooxidans on Granulated Activated Carbon (GAC) capable of oxidising ferrous [Fe (II)] to ferric [Fe (III)]. The Fe (III) significantly converts the As (III) to As (V) and ultimately removed greater than 95% by the bed of GAC, limestone, and sand. The significant influence of Fe (II) concentration (0.1–1.5 gL−1), flowrate (0.06–0.18 Lh−1), and initial As (III) concentration (100–1000 μgL−1) on the arsenic removal efficiency was investigated. The simulated water sample containing the different concentration of As (III) and other ions was used in the study. The removal of other co-existing ions present in contaminated water was also investigated in column study. The concentration of arsenic was found to be <10 μgL−1 which is below Maximum Contaminant Level (MCL) as per WHO in treated water. The results confirmed that the present system including adsorptive-filtration was successfully used for the treatment of contaminated water containing As (III) ions.
|
