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Nanocellulose for biosorption of chlorpyrifos from water: chemometric optimization, kinetics and equilibrium(Cellulose )
(Pareshkumar G. Moradeeya , Madhava Anil Kumar , Ravikumar B. Thorat, Manali Rathod , Yasmin Khambhaty ,,,1319–1332,Year : 2017)
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The study explores the biosorption potential of nanocellulose (NC) to remove an insecticide, chlorpyrifos (CP), from aqueous solutions using the batch method. Biosorption kinetics were very fast and reached equilibrium in 60 min, and the experimental kinetic data had fit well with the pseudo-second-order model. Film diffusion was the rate-limiting step for the biosorption of CP onto crystalline nanocellulose (CNC). The equilibrium sorption was well described by the Sips and Langmuir isotherm models. The values of maximum sorption capacities (7.237–5.017 mg/g for the Sips and 12.325–7.247 mg/g for the Langmuir model) decreased with an increase in temperature from 288 to 308 K, signifying biosorption of CP is an exothermic process. Based on the central composite design (CCD), two-factor interaction (2FI) and quadratic models, the correlation between the effects of variable parameters on the CP biosorption onto NC was evaluated. The chemometric analyses suggested that 1.5 g/l NC required 20 min to biosorb 5 mg/l CP to yield an efficiency of 99.3%. Overall, the results demonstrated that NCs can be a promising biosorbent for the removal of pesticides from aqueous streams.
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Integrative genomic analysis identifies ancestry-related expression quantitative trait loci on DNA polymerase β and supports the association of genetic ancestry with survival disparities in head and neck squamous cell carcinoma(Cancer)
(Meganathan, P. R., Devarajan, K., Blackman, E., Gibbs, D., Luce, D., Deloumeaux, J., Duflo, S., Liu, J.C., Mehra, R., Kulathinal, R.J., Ragin, C.C,,,849-860,Year : 2017)
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BACKGROUND:
African Americans with head and neck squamous cell carcinoma (HNSCC) have a lower survival rate than whites. This study investigated the functional importance of ancestry-informative single-nucleotide polymorphisms (SNPs) in HNSCC and also examined the effect of functionally important genetic elements on racial disparities in HNSCC survival.
METHODS:
Ancestry-informative SNPs, RNA sequencing, methylation, and copy number variation data for 316 oral cavity and laryngeal cancer patients were analyzed across 178 DNA repair genes. The results of expression quantitative trait locus (eQTL) analyses were also replicated with a Gene Expression Omnibus (GEO) data set. The effects of eQTLs on overall survival (OS) and disease-free survival (DFS) were evaluated. RESULTS: Five ancestry-related SNPs were identified as cis-eQTLs in the DNA polymerase (POLB) gene (false discovery rate [FDR]0.01). The homozygous/heterozygous genotypes containing the African allele showed higher POLB expression than the homozygous white allele genotype (P<.001). A replication study using a GEO data set validated all 5 eQTLs and also showed a statistically significant difference in POLB expression based on genetic ancestry (P=.002). An association was observed between these eQTLs and OS (P<.037; FDR<0.0363) as well as DFS (P=.018 to .0629; FDR 0.079) for oral cavity and laryngeal cancer patients treated with platinum-based chemotherapy and/or radiotherapy. Genotypes containing the African allele were associated with poor OS/DFS in comparison with homozygous genotypes harboring the white allele.
CONCLUSIONS:
Analyses show that ancestry-related alleles could act as eQTLs in HNSCC and support the association of ancestry-related genetic factors with survival disparities in patients diagnosed with oral cavity and laryngeal cancer. Cancer 2017;123:849-60. © 2016 American Cancer Society.
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Sensitivity of PBL Schemes of WRF-ARW Model in Simulating Boundary Layer Flow Parameters for its Application to Air Pollution Dispersion Modelling over a Tropical Station(Atmosphere)
(Boadh, R., Satyanarayana, A.N.V., and Madala,,,, 61-81,Year : 2016)
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Mesoscale atmospheric circulations play an important role in the transport of air pollution and local air quality issues. The planetary boundary layer (PBL), the thermo-dymamical structure and the flow field play an important role in air pollution dispersion. Hence, the PBL parameters over Nagpur, India are simulated using the ARW v. 3.6.1 mesoscale model. High-resolution simulations are conducted with triple nested domains having a horizontal resolution of 27, 9 and 3 km, as well as 27 vertical levels by using the 1 × 1° NCEP Final Analysis meteorological fields for initial and boundary conditions. Eight fair-weather days in winter and summer (January and April 2009) with no significant synoptic activity were chosen for the study. Sensitivity experiments of the ARW model were conducted with two non-local (Yonsei University [YSU], and Asymmetric Convective Model v. 2 [ACM2]) and three local turbulence kinetic energy (TKE) closure (Mellor-Yamada Nakanishi and Niino Level 2.5 PBL [MYNN2], Mellor-Yamada-Janjic [MYJ], and quasi-normal scale elimination [QNSE]) turbulence diffusion parameterizations, to study the evolution of PBL parameters and the thermodynamical structure during the study period. After validation of the simulated parameters with the available in situ data, it was revealed that the non-local PBL scheme YSU, followed by local scheme MYNN2, could be able to capture the characteristic variations of surface meteorological variables and the thermodynamical structure of the atmosphere. The present results suggest that PBL schemes, namely YSU and MYNN2, performed better in representing the boundary-layer parameters and are useful for air pollution dispersion studies.
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Ancestral-derived effects on the mutational landscape of laryngeal cancer(Genomics)
(Meganathan, P. R., Rob J. Kulathinal, Yujin Chung, Ilya Serebriiskii, Jeffrey Liu,Camille C. Ragin
,,,76-82,Year : 2016)
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Laryngeal cancer disproportionately affects more African-Americans than European-Americans. Here, we analyze the genome-wide somatic point mutations from the tumors of 13 African-Americans and 57 European-Americans from TCGA to differentiate between environmental and ancestrally-inherited factors. The mean number of mutations was different between African-Americans (151.31) and European-Americans (277.63). Other differences in the overall mutational landscape between African-American and European-American were also found. The frequency of C>A, and C>G were significantly different between the two populations (p-value < 0.05). Context nucleotide signatures for some mutation types significantly differ between these two populations. Thus, the context nucleotide signatures along with other factors could be related to the observed mutational landscape differences between two races. Finally, we show that mutated genes associated with these mutational differences differ between the two populations. Thus, at the molecular level, race appears to be a factor in the progression of laryngeal cancer with ancestral genomic signatures best explaining these differences.
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Removal of m-phenylenediamine by adsorption onto activated carbon: Kinetics, equilibrium and process design(Desalination and Water Treatment)
(Patel, M. Rathod, K. H. Mody,57 (9),,4205-4219,Year : 2016)
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The adsorption behavior of m-phenylene diamine (m-PDA) from aqueous solution onto activated carbon was investigated under various experimental conditions, such as contact time, adsorbate concentration, and temperature. Maximum adsorption capacity for m-PDA was found to be 33.17 mg?g?1 at pH 7.0 and temperature 303 K. The adsorption kinetics data were best described by the pseudo-second-order rate equation and the equilibrium was achieved after 120 min. The m-PDA adsorption was governed by film diffusion process. Besides, equilibrium data were very well represented by the Redlich–Peterson model. A model for prediction of the dose of adsorbent required to achieve a range of m-PDA removals for a given number of adsorption–desorption cycles has been developed and validated based on the Langmuir isotherm. Thermodynamic parameters indicated the spontaneous, endothermic, and increased random nature of m-PDA adsorption. The amide, carboxylic acid, and nitro groups of the activated carbon were involved in chemical interaction with the m-PDA molecules. Results suggested that the activated carbon has good potential for remediation of m-PDA contaminated waters.
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Sensitivity of PBL Parameterization schemes of Weather Research Forecasting Model and coupling with AERMOD in the dispersion of NOX over Visakhapatnam (India)(Asia-Pacific Journal of Chemical Engineering)
(R.Boadh, A NAV Satyanarayana, S. Madala,,,356-368,Year : 2015)
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An attempt has been made to develop a pre?processor coupler for offline coupling of atmospheric mesoscale model (WRF) with a dispersion model (AERMOD) to estimate the ground level concentrations due to industrial sources over a coastal city, Visakhapatnam, India. To integrate AERMOD in standalone mode, surface and upper meteorological observations and various planetary boundary layer (PBL) parameters with good temporal resolution are required, that are absent for most locations in India. We have developed an integrated WRF?AERMOD modeling system that gets required PBL parameters the pre?processor coupler. Using this model, evaluation of the dispersion of nitrogen oxides (NOX) over Visakhapatnam during summer month with emission inventory of elevated point sources of various industries is conducted. Sensitivity studies of PBL schemes of WRF after validation reveal that the non?local schemes, Yonsei University (YSU) followed by Asymmetric Convective Model version 2 (ACM2), captured well the characteristic variations of surface meteorological variables, vertical atmospheric structure. After validating with monitored air quality data, it is found that the integrated model with YSU followed by ACM2 is good in simulating ground level concentrations of NOX. The present work advocates that the developed modeling system is useful for better assessment of pollution dispersion over coastal regions. © 2015 Curtin University of Technology and John Wiley & Sons, Ltd.
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A 454 sequencing approach to dipteran mitochondrial genome research.(Genomics)
(Meganathan, B. S., Wells, J.D., Guerrero, F., Ray, D. A. ,,, 53-60,Year : 2015)
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The availability of complete mitochondrial genome (mtgenome) data for Diptera, one of the largest metazoan orders, in public databases is limited. The advent of high throughput sequencing technology provides the potential to generate mtgenomes for many species affordably and quickly. However, these technologies need to be validated for dipterans as the members of this clade play important economic and research roles. Illumina and 454 sequencing platforms are widely used in genomic research involving non-model organisms. The Illumina platform has already been utilized for generating mitochondrial genomes without using conventional long range PCR for insects whereas the power of 454 sequencing for generating mitochondrial genome drafts without PCR has not yet been validated for insects. Thus, this study examines the utility of 454 sequencing approach for dipteran mtgenomic research. We generated complete or nearly complete mitochondrial genomes for Cochliomyia hominivorax, Haematobia irritans, Phormia regina and Sarcophaga crassipalpis using a 454 sequencing approach. Comparisons between newly obtained and existing assemblies for C. hominivorax and H. irritans revealed no major discrepancies and verified the utility of 454 sequencing for dipteran mitochondrial genomes. We also report the complete mitochondrial sequences for two forensically important flies, P. regina and S. crassipalpis, which could be used to provide useful information to legal personnel. Comparative analyses revealed that dipterans follow similar codon usage and nucleotide biases that could be due to mutational and selection pressures. This study illustrates the utility of 454 sequencing to obtain complete mitochondrial genomes for dipterans without the aid of conventional molecular techniques such as PCR and cloning and validates this method of mtgenome sequencing in arthropods.
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Nanocrystalline cellulose for removal of tetracycline hydrochloride from water via biosorption: Equilibrium, kinetic and thermodynamic studies(Ecological Engineering)
(M.Rathod, S. Halder and ShaikBasha,84,,240-249,Year : 2015)
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Crystalline nanocellulose, synthesized from green seaweed, Ulva lactuca, was investigated for the sorption of tetracycline hydrochloride (TC) from aqueous solutions. The effects of pH, contact time, concentration of TC and desorption were studied in batch mode. The kinetics of TC biosorption was very fast and reached equilibrium in almost 2 h. The kinetic data was well described by double exponential and the pseudo-second-order kinetic models. Film diffusion is the rate-limiting step for the biosorption of TC on to crystalline nanocellulose. Both Redlich–Peterson (R–P) and Polanyi–Manes (P–M) isotherm models described the equilibrium data well. The values of maximum sorption capacities (6.48–7.73 mg/g for R–P and 6.57–7.96 mg/g for P–M models) increased with temperature. A thermodynamic study (?H° = 22.64 kJ/mol, ?S° = 9.85 kJ/(mol/K)) demonstrated that biosorption of TC is spontaneous, endothermic and an entropy-driven process. The reusability of sorbent was achieved by HNO3 (5 w/w%) with desorption efficiency of 95.20% in 4 h. The overall results have significant implications for the removal of TC from aqueous solution with crystalline nanocellulose.
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Sensitivity of PBL Parameterization schemes of Weather Research Forecasting Model and coupling with AERMOD in the dispersion of NOX over Visakhapatnam(Journal of Chemical Engineering)
(R.Boadh, A.N.V. Satyanarayana, T.V.B.P.S. Rama Krishna and M. Srikanth,10(3),,356-368,Year : 2015)
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No information is available
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UV induced photocatalytic degradation of aqueous acetaminophen: the role of adsorption and reaction kinetics(Environmental Science and Pollution Research)
( David Keane, Kieran Nolan, Michael Oelgemoller, Jenny Lawler, John M. Tobin, Anne Morrissey,22,,2219-2230,Year : 2015)
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Nanostructured titania supported on activated carbon (AC), termed as integrated photocatalytic adsorbents (IPCAs), were prepared by ultrasonication and investigated for the photocatalytic degradation of acetaminophen (AMP), a common analgesic and antipyretic drug. The IPCAs showed high affinity towards AMP (in dark adsorption studies), with the amount adsorbed proportional to the TiO2 content; the highest adsorption was at 10 wt% TiO2. Equilibrium isotherm studies showed that the adsorption followed the Langmuir model, indicating the dependence of the reaction on an initial adsorption step, with maximum adsorption capacity of 28.4 mg/g for 10 % TiO2 IPCA. The effects of initial pH, catalyst amount and initial AMP concentration on the photocatalytic degradation rates were studied. Generally, the AMP photodegradation activity of the IPCAs was better than that of bare TiO2. Kinetic studies on the photocatalytic degradation of AMP under UV suggest that the degradation followed Langmuir–Hinshelwood (L–H) kinetics, with an adsorption rate constant (K) that was considerably higher than the photocatalytic rate constant (k r), indicating that the photocatalysis of AMP is the rate-determining step during the adsorption/photocatalysis process.
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Biosorption of ammoniacal nitrogen (N-NH4+) from aqueous solutions with low cost biomaterials: Kinetics and optimization of contact time(The International Journal of Environmental Science and Technology)
(N Mansuri, Kalpana Mody,,,1711-1722,Year : 2014)
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The biosorption of ammoniacal nitrogen (NNH4 ) from aqueous solutions by dead biomass of brown seaweed Cystoseira indica and Jatropha oil cake (JOC), which is generated in the process of biodiesel recovery from its seeds, was studied under diverse experimental conditions. The N-NH4 biosorption was strictly pH dependent, and maximum uptake capacity of C. indica (15.21 mg/g) and JOC (13.59 mg/g) was observed at initial pH 7 and 3, respectively. For each biosorbent–N-NH4 system, kinetic models were applied to the experimental data to examine the mechanisms of sorption and potential rate-controlling steps. The generalized rate model and pseudo-second-order kinetic models described the biosorption kinetics accurately, and the sorption process was found to be controlled by pore and surface diffusion for these biosorbents. Results of four-stage batch biosorber design analysis revealed that the required time for the 99 % efficiency removal of 40 mg/L N-NH4 from 500 L of aqueous solution were 76 and 96 min for C. indica and JOC, respectively. The Fourier transform infrared spectroscopy analysis before and after biosorption of ammonium onto C. indica and JOC revealed involvement of carboxylic and hydroxyl functional groups.
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Occurrence and distribution of selected heavy metals and boron in ground water of Gulf of Khambhat region, Gujarat, India(Environl Sci Pollut Res)
(D. Upadhyaya, M.D Survaiya, S.Mandal, R. B. Thorat, S. Haldar, H.Dave, K.Baxi, R.H Trivedi and Kalpana Mody,,,3880-3890,Year : 2014)
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The concentration of selected heavy metals, like As, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn as well as B, was measured by inductively coupled plasma-optical emission spectrometry (ICP-OES) in groundwater samples from various locations in the Gulf of Khambhat (GoK), an inlet of the Arabian Sea in the state of Gujarat, India, during post-monsoon, winter, and pre-monsoon seasons in a year. Most heavy elements are characterized by low mobility under slightly alkaline and reducing conditions; concentrations in confined aquifers are smaller than the maximum permissible values for drinking water. The temporal changes indicate that a majority of metals is entering the aquifer during monsoon. Principle component analysis of the heavy metal data suggests that Co, Cu, Cd, and Zn are interrelated with each other and derived significantly from anthropogenic route, while input of Pb and Cr may be due to atmospheric deposition in the study area. Both weathering of rocks and anthropogenic input were found to be main sources of elements in the groundwater. The heavy metal levels in groundwaters of the GoK region in comparison with some of the European and Asian sites were higher; however, these metal levels were found to be comparable with few urban sites in the world.
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Water pollution of Sabarmati River- A Harbinger to Potential Disaster( Environment Monitoring and Assessment)
(Soumya Haldar, Subir Kumar Mandal, R. B. Thorat, Krushnakant Baxi, Navalsang Parmer, Vipul Patel, K.H. Mod
,,,2231-2242,Year : 2014)
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River Sabarmati is one of the biggest and major river of Gujarat that runs through two major cities of Gujarat, Gandhinagar and Ahmedabad and finally meets the Gulf of Khambhat (GoK) in the Arabian Sea. A study was conducted to evaluate the water quality of this river, as it could possibly be one of the major sources for filling up Kalpasar, the proposed man-made freshwater reservoir supposed to be the biggest one in the world. A total of nine sampling stations were established covering 163 km stretch of the river from upstream of Gandhinagar city to Vataman near Sabarmati estuary. Physicochemical (temprature, pH, salinity, chloride, total dissolved solids, turbidity, dissolved oxygen, biochemical oxygen demand, phenol, and petroleum hydrocarbons), biological (phytoplankton), and microbiological (total and selective bacterial count) analyses indicated that the river stretch from Ahmedabad-Vasana barriage to Vataman was highly polluted due to perennial waste discharges mainly from municipal drainage and industries. An implementation of sustainable management plan with proper treatment of both municipal and industrial effluents is essential to prevent further deterioration of the water quality of this river.
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Efficient removal of phosphate from aqueous solutions by red seaweed, Kappaphycus alverezii(J Cleaner Prod )
(M Rathod, Kalpana Mody,,,484-493,Year : 2014)
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In the present study, dead biomass of marine macro algae, Kappaphycus alverezii (K. alverezii) was used for the removal of phosphate from water. The effect of different parameters such as contact time, adsorbate concentration, biosorbent dose and temperature was investigated. The biosorption kinetics data were best described by the pseudo-second order rate equation, and equilibrium was achieved after 80 min. The phosphate biosorption was governed by film diffusion process. The maximum phosphate biosorption on K. alverezii was 59.77 mg/g at 25 °C and it followed the Redlich-Peterson model. The biosorption on K. alverezii increased with increasing temperature and was the highest at pH 6.0. The calculated thermodynamic parameters (?G°, ?H° and ?S°) showed that the biosorption of phosphate onto K. alverezii biomass was feasible, spontaneous and endothermic at 15–45 °C. Results suggest that the prepared biomass of K. alverezii has potential in remediation of phosphate contaminated waters.
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Synthesis of acetone reduced graphene oxide/Fe3O4 composite through simple and efficient chemical reduction of exfoliated graphene oxide for removal of dye from aqueous solution(Journal of Materials Science)
(K Parmar, Isha Patel, ZVP Murthy
,,,6772-6783,Year : 2014)
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A simple and effective technique for reduction of graphene oxide at low temperature (70 °C) using acetone was reported for the first time. Magnetically recoverable acetone reduced graphene oxide (ARGO)/Fe3O4 composite was synthesized by uniformly decorating Fe3O4 on ARGO. The synthesized ARGO/Fe3O4 composite was characterized by the powder X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analysis. An organic dye rhodamine 6G was used as an adsorbate for investigating the adsorption characteristics of the composite. The adsorption kinetic data were best described by the pseudo-second-order model, and equilibrium was achieved within 2 h. Dye adsorption was favored in basic conditions (pH 9–11) and governed by intraparticle diffusion process. The maximum dye adsorption on the composite was 93.37 mg/g at 293 K, and it followed the Langmuir–Freundlich model. The calculated thermodynamic parameters (?G°, ?H° and ?S°) showed that the dye adsorption onto composite was feasible, spontaneous and exothermic. The ARGO/Fe3O4 composite was easily controlled in magnetic field for desired separation, leading to an easy removal of the dye from wastewater, which holds great potential for dye decontamination.
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Multiple lineages of ancient CR1 retroposons shaped the early genome evolution of amniotes(Genome Biology and Evolution)
(Suh, A., Churakov, G., Meganathan, P. R., Platt, R. N., Jurka, J., Kojima, K. K., Caballero, J., Smit, A. F., Vliet, K. A., Hoffmann, F. G., Brosius, J., Green, R. E., Braun, E. L., Ray, D. A., Schmitz, J.
,,,205-217,Year : 2014)
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Chicken repeat 1 (CR1) retroposons are long interspersed elements (LINEs) that are ubiquitous within amniote genomes and constitute the most abundant family of transposed elements in birds, crocodilians, turtles, and snakes. They are also present in mammalian genomes, where they reside as numerous relics of ancient retroposition events. Yet, despite their relevance for understanding amniote genome evolution, the diversity and evolution of CR1 elements has never been studied on an amniote-wide level. We reconstruct the temporal and quantitative activity of CR1 subfamilies via presence/absence analyses across crocodilian phylogeny and comparative analyses of 12 crocodilian genomes, revealing relative genomic stasis of retroposition during genome evolution of extant Crocodylia. Our large-scale phylogenetic analysis of amniote CR1 subfamilies suggests the presence of at least seven ancient CR1 lineages in the amniote ancestor; and amniote-wide analyses of CR1 successions and quantities reveal differential retention (presence of ancient relics or recent activity) of these CR1 lineages across amniote genome evolution. Interestingly, birds and lepidosaurs retained the fewest ancient CR1 lineages among amniotes and also exhibit smaller genome sizes. Our study is the first to analyze CR1 evolution in a genome-wide and amniote-wide context and the data strongly suggest that the ancestral amniote genome contained myriad CR1 elements from multiple ancient lineages, and remnants of these are still detectable in the relatively stable genomes of crocodilians and turtles. Early mammalian genome evolution was thus characterized by a drastic shift from CR1 prevalence to dominance and hyperactivity of L2 LINEs in monotremes and L1 LINEs in therians.
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Three crocodilian genomes reveal ancestral patterns of evolution among archosaurs(SCIENCE)
(Green, R. E., Meganathan P. R., Ray. D. A,346(6215):124449.,,,Year : 2014)
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To provide context for the diversification of archosaurs--the group that includes crocodilians, dinosaurs, and birds--we generated draft genomes of three crocodilians: Alligator mississippiensis (the American alligator), Crocodylus porosus (the saltwater crocodile), and Gavialis gangeticus (the Indian gharial). We observed an exceptionally slow rate of genome evolution within crocodilians at all levels, including nucleotide substitutions, indels, transposable element content and movement, gene family evolution, and chromosomal synteny. When placed within the context of related taxa including birds and turtles, this suggests that the common ancestor of all of these taxa also exhibited slow genome evolution and that the comparatively rapid evolution is derived in birds. The data also provided the opportunity to analyze heterozygosity in crocodilians, which indicates a likely reduction in population size for all three taxa through the Pleistocene. Finally, these data combined with newly published bird genomes allowed us to reconstruct the partial genome of the common ancestor of archosaurs, thereby providing a tool to investigate the genetic starting material of crocodilians, birds, and dinosaurs.
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Application of graphene oxide as a hydrothermal catalyst support for synthesis of TiO2 whiskers(Chemical Communications)
(K Parmar, Shaik Basha, ZVP Murthy,50 (95),,15010-15013,Year : 2014)
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How graphene oxide (GO) with its step edges and wrinkles (?1–2 nm) acts as a catalyst in hydrothermal nucleation and growth is demonstrated. TiO2 whiskers were prepared by using GO as a support, while TiO2 nanograss was prepared without using GO.
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Assessment of Dispersion of Oxide of Nitrogen Using AERMOD over a Tropical Industrial Region(International Journal of Computer Applications)
(Boadh, R., Satyanarayana, A.N.V., and Rama Krishna, T.V.B.P.S,90,,43-50,Year : 2014)
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Accurate representation of dispersion of air pollutants is essential for environmental management and planning purposes. In the present study, an attempt has been made to investigate the improvement of pollution dispersion using AERMOD model which includes atmospheric boundary layer processes explicitly. Ranchi region, a fast growing urban city with industries and vehicular pollution in the Jharkhand state of India is chosen. Surface micro-meteorological tower data and upper air radiosonde observations are utilized in the study. Surface boundary layer parameters such as friction velocity and sensible heat flux used as input to AERMOD model are obtained from flux-profile relationships and validated with turbulence measurements. The pollutant concentrations includes industrial as well as vehicular sources predicted by AERMOD are validated with the ambient air quality data of Central Pollution Control Board at Ranchi. Results reveal that AERMOD performed well in representing air pollution dispersion over Ranchi region.
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Novel microsatellite DNA markers for Indian Gharial (Gavialis gangeticus).(Conservation Genetics Resources)
(Jogayya, K. N., Meganathan, P. R., Dubey, B., Haque.I,,, 787-790,Year : 2013)
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The present status of existing crocodile species has increased the necessity to develop novel utilities for conservation. We have developed 18 microsatellite loci from the Indian Gharial (Gavialis gangeticus) intended for genomic explanation and applied them to study genetic variation. Polymorphism of each locus was assessed in 32 individuals for G. gangeticus India. The number of alleles per locus varied from 2 to 8 (mean 5.5) for Indian population. Observed and expected heterozygosity ranged from 0.73 to 1.00 and 0.50 to 0.81, respectively and the average polymorphic information content is 0.565. These selected markers are helpful for assessing population structure, intraspecific difference, and conservation and management of G. gangeticus.
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