- 22 December, 2024
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| Designation: |
Senior Scientist
|
| Email Id: | bhuvanesh.s@neeri.res.in |
| Qualification: |
B. Tech. (Industrial Biotechnology), M.S. (Bio-process Engineering), Ph.D. (Biochemical Engineering) |
| Specialization: |
Biological wastewater and sludge treatment
|
| Address: |
Sub-Vertical 3B: Waste Water Management, NEERI, Nagpur
|
| Biodata: |
| Sr. No. | Publication Name |
|---|---|
| 1 |
Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatography
Abundant larval transcript (ALT), a novel filarial protein, has been shown to have great potential as a vaccine in the prevention of human lymphatic filariasis. In this study, we report a method for the production of recombinant ALT-2 protein, expressed in the cytoplasm of bacterium Escherichia coli in soluble form and purification in a single step using hydrophobic interaction chromatography (HIC). Fermentation was done by continuous fed-batch methodology with dissolved oxygen (DO)-controlled feed addition. The culture was induced with 1 mM isopropyl-?-D-thiogalactopyranoside (IPTG). Up to 9 g/l dry cell weight (DCW) of biomass was obtained from 1.6 l of Luria?Bertani (LB) broth in a bench-scale reactor. Around 200 mg/l of purified ALT-2 with a yield of about 60% was obtained. This is almost a 2.5-fold increase in final protein yield compared to purification using immobilized metal affinity chromatography (IMAC). |
| 2 |
Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatograph
Abundant larval transcript (ALT), a novel filarial protein, has been shown to have great potential as a vaccine in the prevention of human lymphatic filariasis. In this study, we report a method for the production of recombinant ALT-2 protein, expressed in the cytoplasm of bacterium Escherichia coli in soluble form and purification in a single step using hydrophobic interaction chromatography (HIC). Fermentation was done by continuous fed-batch methodology with dissolved oxygen (DO)-controlled feed addition. The culture was induced with 1 mM isopropyl-?-D-thiogalactopyranoside (IPTG). Up to 9 g/l dry cell weight (DCW) of biomass was obtained from 1.6 l of Luria–Bertani (LB) broth in a bench-scale reactor. Around 200 mg/l of purified ALT-2 with a yield of about 60% was obtained. This is almost a 2.5-fold increase in final protein yield compared to purification using immobilized metal affinity chromatography (IMAC). |
| 3 |
Optimization of medium for the production of subtilisin from Bacillus subtilis MTCC 441
Subtilisins (E.C.3.4.21.62) are alkaline proteases that are secreted by members of the genus Bacillus. They are serine proteases that exhibit high specific activity on proteinaceous substrates, function optimally at moderate temperatures, and are stable under alkaline conditions. Thus the use of subtilisin as an enzyme additive could help in development of quality laundry detergents. In this work the subtilisin production from Bacillus subtilis (MTCC 441) was improved by altering and optimizing the media components. This alteration was brought up by process development strategy. The effect of yeast extract, casein, peptone and sodium chloride on subtilisin production was studied and were optimized using Box Behnken Design. The optimal growth conditions for B. subtilis MTCC 441 were found to 37 o C, and pH 7.5. The optimal media composition for subtilisin production was found to be yeast extract at 6.75 g/L, peptone at 4.41 g/L, sodium chloride at 6.08 g/L, casein at 10.75 g/L with glucose at 5 g/L. The predicted and observed response were 181.00 U/mg (with desirability =0.87) and 185.70 U/mg, respectively. |
| 4 |
Three-stage biological system for treatment of coke oven effluent
A three-stage continuous biological treatment system has been developed for removal of cyanide, phenol, organics, and ammonia from a coke oven effluent. Two stages of activated sludge treatment with completely mixed aeration tanks followed by a hybrid anoxic reactor, in series, were used. The hybrid anoxic reactor uses self-immobilized microbial biomass (granular sludge) under fluidized conditions. A 50??L/day pilot plant was evaluated for a period of over 6 months to monitor organics, ammonia, phenol, and cyanide removal. The activated sludge treatment systems were operated at a hydraulic retention time of 1 day and the hybrid reactor at 16 h. Major constituents of the effluent used in the study were cyanide (9–33??mg/L), phenol (21–235??mg/L), ammoniacal nitrogen (400–1,600??mg?NH3-N/L), organic load [400–1,500 mg chemical oxygen demand (COD)/L], and nitrate nitrogen (0–90??mg?NO3-N/L). Removal efficiencies were around 90% for organic load, 88% for ammoniacal nitrogen, 100% for nitrate nitrogen, and almost 99 and 100% for cyanide and phenol, respectively. The process is presently being scaled up to 1,000 L per day. |
| 5 |
Rapid Granulation and Start-up of a Hybrid Anoxic Reactor for Biological Denitrification
Rapid granulation of biomass and reactor start-up has been studied in a novel denitrifying reactor. The effect of wastewater characteristics, reactor operating conditions and reactor geometry on microbial granulation has been studied. It was possible to achieve granulation in just 15 days of reactor start-up. In 15 days the settling velocity of the granules was 1.5 cm s-1, which is almost 10 folds higher than that of seed sludge. The reactor was able to handle a nitrate loading rate of 50 g NO3-N m-3 day-1 in 3 days of reactor start-up with rates reaching up to 460 g NO3-N m-3 day-1 in just 30 days of reactor start-up with a removal efficiency of almost 100%. Based on the experimental observation, a hypothesis for the cause of rapid granulation has been proposed. |
View Biodata [0.36 MB]