- 22 December, 2024
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| Designation: |
Principal Scientist
|
| Email Id: | s_pal[at]neeri[dot]res[dot]in |
| Qualification: |
M.Sc.(Chem), PhD (Pharmacy-Nanobiotech) |
| Specialization: |
Chemistry
|
| Address: |
Sub-Vertical 3B: Waste Water Management, NEERI, Nagpur
|
| Biodata: |
| Sr. No. | Project Name |
|---|---|
| 1 |
Indo-French Water Networking on SWACHH NEER - UNE VIE MEILLEURE
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| 2 |
Environmental Impact Assessment of Four Lanning of Boulevard for Alleviated Highway - Dal Corridor Project
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| 3 |
Study on Pollution Status of River Dravyavati and Development of Pollution Abatement Strategies for Rejuvenation of the River System
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| 4 |
Recovery of Hydrochloric Acid for Process Reuse and Calcium Sulphate or Calcium Chloride as Marketable Byproducts from High TDS Process Effluent of Dicalcium Phosphate Manufacturing at Bamni Proteins Limited, Chandrapur
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| 5 |
Sustainable Grey Water (GW) treatment technology for community building
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| 6 |
Design & Synthesis of Colorimetric/Fluorometric Chemosensors for Selective Detection of Hazardous Cations, Anions and Nerve Agents with Projected Environmental Applications
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| 7 |
Management and Restoration Plan for Sambhar Lake, Rajasthan
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| 8 |
Removal of Heavy Metal Residuals and Sedimentation of Laterite Muds Flocculated by Polymers
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| 9 |
Adequacy and Efficacy Assessment of STPs in Srinagar & Water Quality Assessment of Dal-Nigeen Lake
|
| 10 |
Wastewater Management Plan for Malanjkhand Copper Project
|
| Sr. No. | Publication Name |
|---|---|
| 1 |
Sustainable Governance of Groundwater as Drinking Water in the Post Covid-19 World: An Urban Water Security Perspective
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| 2 |
Utilization of layered double hydroxides (LDHs) and their derivatives as photocatalysts for degradation of organic pollutants
Direct or indirect discharge of wastes containing organic pollutants have contributed to the environmental pollution globally. Decontamination of highly polluted natural resources such as water using an effective treatment is a great challenge for public health and environmental protection. Photodegradation of organic pollutants using efficient photocatalyst has attracted extensive interest due to their stability, effectiveness towards degradation efficiency, energy, and cost efficiency. Among various photocatalysts, layered double hydroxides (LDHs) and their derivatives have shown great potential towards photodegradation of organic pollutants. Herein, we review the mechanism, key factors, and performance of LDHs and their derivatives for the photodegradation of organic pollutants. |
| 3 |
Novel photocatalytic techniques of Organic dye degradation in water
Around 11 million tons of water is polluted per year and dye stuff industries contribute ?20% of the total industrial water pollution. Dyes are the recalcitrant pollutants in water because of their complex aromatic structure, high molecular weight, stability, degradation resistance, carcinogenic and mutagenic nature, and tendency to screen sunlight inhibiting photosynthetic reactions. Adsorption and filtration (reverse osmosis using membranes) have always been considered as efficient and economic dye removal methods that can be effortlessly scaled-up in wastewater treatment plants. However, due to high stability and recalcitrant nature of organic dyes these general treatment methods often suffer from low level of efficiency. Given these complexities, in past few decades photocatalytic degradation has grabbed much attention for organic dye removal reflecting its ability for complete mineralization. |
| 4 |
Photo-Induced Synthesis of Coral-Like Hierarchical Ag
Herein, we report, for the first time, rapid and facile photo-induced synthesis of hierarchical Ag?Fe bimetallic nanostructures with tailorable morphologies based on variation of Fe2+ concentrations at the submillimolar level. The synthesis methodology relies on the photo-oscillation of the Fe(II)?Fe(III)?Fe(II) system in the aqueous medium, leading to reduction of Ag+ to Ag0 nanoparticles ,wherein Ag0 nanoparticles are stabilized in an Fe-oxide framework. The morphology of the product is tailored from scattered to prominent coral-like nanostructures by simply varying the Fe2+ concentrations at the submillimolar level. The developed method eliminates the essential need of presynthesized precursor metal nanoparticles, toxic templates or surfactants, organic solvents, and sophisticated instrumentations or stringent reaction conditions. |
| 5 |
Potential of Agro-Products in the Green Synthesis of Nano-Metallopharmaceuticals
Recent advancements in nanotechnology have received more attention due to their safer and effective pharmacology and medicinal applications. Compared to traditional drugs, nano-metallopharmaceuticals offer a better-targeted approach to minimize off-target effects and, thus, undesirable side effects. Despite many potential applications, growing concerns have been expressed for their safe production to reduce antagonistic human health effects. The production of metallopharmaceuticals can be grouped into three classes ? chemical, physical, and biological. However, reactants often used in chemical procedures are toxic, and their remnants, if they exist in nanomedicine, can induce systemic toxicity. Similarly, physical methods are expensive, with high power consumption, and they require a large space. |
| 6 |
Heterolayered TiO2@ layered double hydroxide-MoS2 nanostructure for simultaneous adsorption-photocatalysis of co-existing water contaminants
The highly ordered heterolayered-nanostructure TiO2@Layered double hydroxide/Molybdenum disulfide (TiO2@LDH/MoS2; T@L/M) is formed by electrostatically-driven self-assembly of positively charged TiO2-dispersed-LDH layers (T@L) and negatively charged MoS2 nanosheets. TiO2 are incorporated in-situ across the LDH during synthesis yielding exfoliated TiO2-dispersed-LDH layers (T@L). Prior to MoS2 assembly, its interlayer spacing is enlarged and defects are created in basal-plane exposing sulfur-rich sites. Visible-light-active MoS2 and TiO2 synthesized heterostructure extending the T@L/M photoactivity in visible spectrum (2.92 eV) for enhanced degradation capacity |
| 7 |
Adsorption of five emerging contaminants on activated carbon from aqueous medium: kinetic characteristics and computational modeling for plausible mechanism
Pharmaceuticals and personal care products (PPCPs) do not have standard regulations for discharge in the environment and are categorized as contaminants of emerging concern as they pose potential threats to ecology as well as humans even at low concentrations. Conventional treatment processes generally employed in the wastewater treatment plants are not adequately engineered for effective removal of PPCPs. Identifying cost-effective tertiary treatment is therefore, important for complete removal of PPCPs from wastewater prior to discharge or reuse. Present study demonstrates adsorption using granular-activated carbon (GAC) as a possible tertiary treatment for simultaneous removal of five PPCPs from aqueous media. Adsorbent was characterized in terms of morphology, surface area, surface charge distribution, and presence of functional groups. |
| 8 |
Profiling of emerging contaminants and antibiotic resistance in sewage treatment plants: an Indian perspective
In India, sewage (partially-treated/ untreated) is randomly used for irrigation because of easy availability and presence of residual organics and nutrients. However, data on the occurrence of contaminants of emerging concerns (CECs) such as pharmaceuticals and personal care products (PPCPs) and antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) in sewage is scarce in Indian perspective. Herein, for the first time, we present a quantitative contamination profiling of selected PPCPs and antibiotic resistance in untreated and biologically-treated sewage from three different sewage treatment plants, located in northern and central part of India. Profiling of PPCPs were done using LC-ESI-MS/MS whereas antibiotic resistance was analyzed using gradient PCR and qPCR techniques. |
| 9 |
SARS-CoV-2: fate in water environments and sewage surveillance as an early warning system
Coronavirus disease has emerged as one of the greatest threats to human well-being. Currently, the whole world is fighting against this pandemic that is transmitted either through exposure to virus laden respiratory or water droplets or by touching the virus contaminated surfaces. The viral load in feces of an infected patient varies according to the severity of the disease. Subsequent detection of viral genome (SARS-CoV-2) in human feces and sewage systems is an emerging concern for public health. This also dictates to reinforce the existing sewage/wastewater treatment facilities. Rapid monitoring is the key to prevent and control the current mass transmission. Wastewater-Based Epidemiology (WBE) is a potential epidemiology tool that can act as a complementary approach for current infectious disease surveillance systems and an early warning system for disease outbreaks |
| 10 |
Corrigendum to
Professor Pal contributed to the conception and design of the study in addition to collecting, analyzing, and interpreting the data and manuscript preparation. e experimental work was carried out in CSIR-National Environmental Engineering Research Institute (CSIR-NEERI) under his supervision. Professor Pal has been added as the corresponding author. |
| 11 |
SARS-CoV-2: sewage surveillance as an early warning system and challenges in developing countries
Transmission of novel coronavirus (SARS-CoV-2) in humans happens either through airway exposure to respiratory droplets from an infected patient or by touching the virus contaminated surface or objects (fomites). Presence of SARS-CoV-2 in human feces and its passage to sewage system is an emerging concern for public health. Pieces of evidence of the occurrence of viral RNA in feces and municipal wastewater (sewage) systems have not only warned reinforcing the treatment facilities but also suggest that these systems can be monitored to get epidemiological data for checking trend of COVID-19 infection in the community. This review summarizes the occurrence and persistence of novel coronavirus in sewage with an emphasis on the possible water environment contamination |
| 12 |
Assessment of Environmental Water Security of an Asian Deltaic Megacity and Its Peri-Urban Wetland Areas
Achieving urban water security requires sustaining the trade-offs between the exploitation of water/environmental resources and ecosystem services. This achievement not only reduces the pollution and contamination in the environment, level of water stress, but also secures good ambient water quality and future for people?s well-being and livelihoods. Changes in land use and land cover and growth of impervious structures can immediately generate severe ecological and social issues and increase the level of natural or manmade risks, affecting the condition of ecosystem services within and in the vicinity of an urban region. As a result of these transformations and further exploitation, due to the growing anthropogenic pressure, surface water and groundwater quality can be deteriorated compared to ambient water quality standards (for both chemical and biological pollutants). Based on land use and land cover (LULC) data retrieved from remote sensing interpretation, we computed the changes of the ecosystem service values (ESV) associated with the LULC dynamics, water quality and, finally, urban water security during the pre-and post-monsoon periods of 2009, 2014 and 2019 in Kolkata, an Asian deltaic megacity, and its peri-urban wetlands named East Kolkata Wetlands (EKW). The area under wetlands reduced comprehensively in 2009?2019 due to the conversion of wetlands into various other classes such as urban settlement, etc. The quality of surface water bodies (such as rivers, lakes, canals and inland wetlands) deteriorated. |
| 13 |
Environmental Disaster Management and Risk Reduction
The environment can be well-defined as all forms that surround both living elements (such as humans or animals, fishes and birds) as well as non-living elements, itself classified as both moving (such as air and water) and non-moving (such as mountains and forests). Human activities do interact with the bio-physical environment in a multifaceted manner connecting different geographical levels. The existence of all living forms on this planet gradually evolved to build a healthy and well-balanced environment throughout the space and time. The new economic demand should operate in a way that shields the biophysical environment to maintain the balanced and sustainable growth both at the present and in the future. |
| 14 |
Effect of Grewia spp. biopolymer on floc properties of coagulated laterite suspension
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| 15 |
Biodegradable Polymeric Nanocarrier-Based Immunotherapy in Hepatitis Vaccination
Various commercial vaccines are used for immunization against hepatitis B. However, these immunotherapeutic vaccines require invasive administration, which can induce side effects, and require multiple shots to elicit an immune response, limiting their efficacy. Compared to traditional hepatitis B vaccines, polymer nanoparticles have more advantageous inherent properties as vaccine delivery carriers, providing increased stability of encapsulated antigen, the possibility of singleshot immunotherapy, and the capability of mucosal administration, which allows various |
| 16 |
Shape-dependent skin penetration of silver nanoparticles: does it really matter?
Advancements in nano-structured materials have facilitated several applications of nanoparticles (NPs). Skin penetration of NPs is a crucial factor for designing suitable topical antibacterial agents with low systemic toxicity. Available reports focus on size-dependent skin penetration of NPs, mainly through follicular pathways. Herein, for the first time, we demonstrate a proof-of-concept study that entails variations in skin permeability and diffusion coefficients, penetration rates and depth-of-penetration of differently shaped silver NPs (AgNPs) via intercellular pathways using both in vitro and in vivo models. The antimicrobial activity of AgNPs is known. |
| 17 |
A multifunctional composite of an antibacterial higher-valent silver metallopharmaceutical and a potent wound healing polypeptide: a combined killing and healing approach to wound care
The present study relates to a combined killing and healing approach for the treatment of infected wounds. Herein we report a multifunctional, including antimicrobial and wound healing, composite containing a conjugate of a bi-valent silver polydiguanide that demonstrated high antibacterial activity in vitro and a potent wound healing polypeptide, histatin-1, for the treatment of infected wounds. The synthesis of silver(II) chlorhexidine [Ag(II)CHX] was accomplished by the oxidation of Ag(I), followed by the complexation of the oxidized metal with chlorhexidine (CHX), whereas the metal complex conjugate of the solid phase-synthesized histatin polypeptide (Hst-1), Hst-1-[Ag(II)CHX], was realized by mixing the starting materials in aqueous solution. |
| 18 |
Feasibility of bioengineered two-stages sequential batch reactor and filtration
In the present study, the feasibility of a bioengineered two-stages sequential batch reactor (BTSSBR) followed by filtration?adsorption process was investigated to treat the agrochemical effluent by overcoming factor affecting process stability such as microbial imbalance and substrate sensitivity. An air stripper stripped 90% of toxic ammonia, and combined with other streams for bio-oxidation and filtration?adsorption. The BTSSBR system achieved bio-oxidation at 6 days hydraulic retention time by fending off microbial imbalance and substrate sensitivity. The maximum reduction in COD and BOD by heterotrophic bacteria in the first reactor was 87% and 90%, respectively. |
| 19 |
Quantitative classification of DNA damages induced by submicromolar cadmium using oligonucleotide chip coupled with lesion-specific endonuclease digestion
Implementation of proper analytical tool for systematic investigation and quantitative determination of different classes of cadmium ion-induced DNA damages, especially at low metal ion concentrations, is still lacking. Using lesion-specific enzymes that cleave DNA at specific classes of damage and a fluorometric approach developed for quantifying fluorophore-labeled oligonucleotides bound to chip surfaces, we determined the frequencies of different lesions (strand breaks, oxidized purines, oxidized pyrimidines, or abasic sites) induced by submicromolar Cd2+. Cd2+-treated oligonucleotide chips were digested with various endonucleases (Fpg protein, endonuclease III, endouclease IV), producing a de novo single strand break (SSB) at their substrate modifications. |
| 20 |
High-content screening of drug-induced cardiotoxicity using quantitative single cell imaging cytometry on microfluidic device
Drug-induced cardiotoxicity or cytotoxicity followed by cell death in cardiac muscle is one of the major concerns in drug development. Herein, we report a high-content quantitative multicolor single cell imaging tool for automatic screening of drug-induced cardiotoxicity in an intact cell. A tunable multicolor imaging system coupled with a miniaturized sample platform was destined to elucidate drug-induced cardiotoxicity via simultaneous quantitative monitoring of intracellular sodium ion concentration, potassium ion channel permeability and apoptosis/necrosis in H9c2(2?1) cell line. Cells were treated with cisapride (a human ether-?-go-go-related gene (hERG) channel blocker), digoxin (Na+/K+-pump blocker), camptothecin (anticancer agent) and a newly synthesized anti-cancer drug candidate (SH-03). |
| 21 |
Pulsed photostimulated-and thermo-luminescence investigations of ? ray-irradiated herbs
In this study, physical detection methods like pulsed photo-stimulated luminescence (PPSL) and thermoluminescence (TL) were investigated to indentify ?-ray irradiation treatment of some medicinal herbs. Dried herbal samples consisting of root, rhizome, cortex, fruit, seed, flower, spike, ramulus, folium and whole plant of nineteen different herbs were irradiated using a 60Co irradiator at 0?50 kGy. Fifteen non-irradiated control samples had photon counts (PCs) less than the lower threshold value (700 counts/60 s). The photon counts of four non-irradiated samples (roots of osterici, angelica gigas, liriope, and whole plant of taraxaci herba) were between the lower and upper threshold values (700?5000 counts/60 s). |
| 22 |
Metallopharmaceuticals based on silver (I) and silver (II) polydiguanide complexes: activity against burn wound pathogens
The in vitro pharmacodynamics of silver(I) and silver(II) complexes of a polydiguanide ligand, chlorhexidine, were assayed to examine the value of the bactericidal endpoint as an alternative means of evaluating their antibacterial activities against burn wound pathogens. |
| 23 |
Development of radiation indicators to distinguish between irradiated and non-irradiated herbal medicines using HPLC and GC-MS
The effects of high dose ?-irradiation on six herbal medicines were investigated using gas chromatography?mass spectrometry (GC/MS) and high-performance liquid chromatography (HPLC). Herbal medicines were irradiated at 0?50 kGy with 60Co irradiator. HPLC was used to quantify changes of major components including glycyrrhizin, cinnamic acid, poncirin, hesperidin, berberine, and amygdalin in licorice, cinnamon bark, poncirin immature fruit, citrus unshiu peel, coptis rhizome, and apricot kernel. No significant differences were found between gamma-irradiated and non-irradiated samples with regard to the amounts of glycyrrhizin, berberine, and amygdalin. However, the contents of cinnamic acid, poncirin, and hesperidin were increased after irradiation. Volatile compounds were analyzed by GC/MS. The relative proportion of ketone in licorice was diminished after irradiation |
| 24 |
Antibacterial Metallopharmaceuticals Based on Nanocrystalline Trivalent Silver Polydiguanide
Synthesis and characterization of nanocrystalline trivalent silver polydiguanide complex was described. The antibacterial activity of the synthesized nanoparticles was evaluated against burn wound pathogens. Synthesis of essentially monodispersed nanoparticles of higher valentmetal complex was accomplished by oxidation of the monovalent silver, followed by stabilization of the oxidized higher-valent metal through complexation with chlorhexidine, a polydiguanide ligand in a reverse microemulsion at room temperature. Their antibacterial potencies wereassessed invitroby determining the MICs and MBCs against four Gram-positive and four Gram-negative bacteria using agar dilution and microdilution methods. |
| 25 |
Synthesis of highly antibacterial nanocrystalline trivalent silver polydiguanide
Highly monodispersed nanoparticles of a trivalent silver polydiguanide complex are synthesized by oxidation of the monovalent silver, followed by stabilization of the oxidized higher-valent metal through complexation with a polydiguanide ligand in a reverse microemulsion at room temperature. The synthesized nanoparticles have excellent photostability and displayed superior antibacterial activity toward Gram-positive and Gram-negative prokaryotes of clinical interest in vitro compared to silver sulfadiazine. |
| 26 |
An IMEA-PDA microchip for on-chip electrochemiluminescence detection
This paper reports the design, fabrication and testing of a microchip wherein interdigitated microelectrode arrays (IMEA) were integrated with bipolar semiconductor photodiode array (PDA) chip to fabricate a highly compact embodiment for on-chip handling of solutions and electrochemiluminescence (ECL) detection. A 12? 12 micro array of photodiodes, each coupled withan interdigitated microelectrode array (IMEA), an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single 2? 2 cm2IC chip. Each photodiode had dimensions of 300? 300?m2 and the photodiode-to-photodiode distance was 100?m2. The chip was successfully applied to the on-chip quantification of electro chemiluminescing probelabeled single stranded oligonucleotides. |
| 27 |
Identification of ?-ray irradiated medicinal herbs by pulsed photostimulated luminescence, thermoluminescence, and electron spin resonance spectroscopy
Dried herbal samples consisting of root, rhizome, cortex, fruit, peel, flower, spike, ramulus, folium, and whole plant of 20 different medicinal herbs were investigated using pulsed photostimulated luminescence (PPSL), thermoluminescence (TL), and electron spin resonance spectroscopy (ESR) to identify ?-ray irradiation treatment. Samples were irradiated at 0-59 kGy using a 60Co irradiator. PPSL measurement was applied as a rapid screening method. control samples of 19 different herbs had photon counts less than the lower and upper threshold values (700counts 60s-1). The photon counts of non-irradiated clematidis radix radix and irradiated evodia and gardenia fruits were between the lower and upper threshold values (700-5,000 counts 60s-1). |
| 28 |
Effects of ?-Ray Irradiation on Armeniacae Semen: A Three-Way Investigation Using GC-MS, HPLC, and Electron Spin Resonance
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| 29 |
Identification of ?-Ray Irradiated Medicinal Herbs by Pulsed Photostimulated Luminescence, Thermoluminescence, and Electron Spin resonance Spectroscopy
Identification of ?-Ray Irradiated Medicinal Herbs by Pulsed Photostimulated Luminescence, Thermoluminescence, and Electron Spin resonance |
| 30 |
DNA Mutation Detection in Microfluidic Devices Using Poly (Nisopropyl) acrylamide As Pseudostationary Phase
Purpose: Recently, capillary electrochromatography (CEC) and mirochip electrochromatography (Microchip CEC) have gained attention due to the wide range of applications based on the use of open tubular capillaries. In this work, the use of phase-separated poly (N-isopropyl) acrylamide (PNIPAM) as a pseudostationary phase for CEC and microchip CEC is demonstrated for the detection of single nucleotide polymorphisms (SNPs). Methods: A typical cross channel microfluidic system was fabricated using an elastomeric material-poly (dimethylsiloxane)(PDMS). A master containing the network of the channels was created using photolithography. PDMS cast against the master yielded a polymeric replica. Conformal contact between the oxygen plasma oxidized surfaces of the PDMS replica and a flat PDMS slab formed the miniaturized capillary electrophoresis system |
| 31 |
Nanocrystalline silver supported on activated carbon matrix from hydrosol: antibacterial mechanism under prolonged incubation conditions
Nanocrystalline silver-supported activated carbon (AC) was fabricated by directly loading silver nanoparticles into the porous AC matrix from a preformed nanosilver hydrosol. Silver-AC composites were also synthesized using a conventional thermal impregnation method. While XRD calculation indicated the presence of Ag crystallites in nanometer range, silver nanoparticle hydrosol-treated AC having the finest crystallite size CS (Escherichia coli. The kinetics of bacterial inactivation, in presence of hydroxyl radical (OH) scavengers, and superoxide anion radical (O? 2) inducer suggest the contribution of the reactive oxygen species (ROS) to antibacterial effect. However, these ROS scavengers did not show any inhibition of bactericidal activity after ?1 h, suggesting that generated ROS are responsible for E. coli inactivation only during the initial 1 h of the incubation time. |
| 32 |
Oligonucleotide chip assay for quantification of gamma ray-induced single strand breaks
An oligonucleotide chip assay was designed for direct quantification of single strand breaks (SSBs) induced by ?-ray irradiation. The oligonucleotides used were 20-mers, which were short enough to produce only a single strand break within a single oligonucleotide. The two ends of the oligonucleotides were labeled with fluorescein and biotin, respectively. The biotinylated ends of the oligonucleotides were immobilized on a silicon wafer chip treated with (3-aminopropyl)triethoxysilane (APTES), glutaraldehyde, and avidin. The DNA fragments cleaved by ?-ray irradiation were detected by a laser-induced fluorescence (LIF) detection system. The ?-ray-induced SSBs were quantified using a calibration curve (fluorescence intensity versus ?-ray dose) without the need for complicated mathematical calculation based on gel-based separation. |
| 33 |
Synthesis, characterization and comparative evaluation of antivacterial activity of diverse morphological silver nanoparticles and higher-valent silver metallopharmaceuticals
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| 34 |
Monitoring Photosensitizer Drug-induced DNA Single Strand Breaks Using Oligonucleotide Chip
This paper describes a novel approach utilizing oligonucleotide chip for determining the photosensitizer-induced DNA single strand breaks (SSBs). Oligonucleotides chips were prepared by immobilizing fluorophore-labeled oligomers on to silicon oxide surfaces. Oligonucleotide surface coverage was determined by alkaline phosphatase (ALP) digestion method, wherein ALP (at high pH; 9.9) led to the cleavage of phosphates in the oligonucleotide backbone, releasing the fluorophore-tagged fragments back into the solution. The fluorescence maximums (measured at 520 nm) of the solutions containing those cleaved fragments were converted to molar concentrations of the fluorescein-modified oligonucleotide by interpolation from a predetermined standard linear calibration curve. |
| 35 |
Monitoring the (photo) genotoxicity of photosensitizer drugs: Direct quantitation of single-strand breaks in deoxyribonucleic acid using an oligonucleotide chip
Oligonucleotide chip-based assays can be a sample-thrifty, time-saving, routine tool for evaluation of chemical-induced DNA strand breaks. This article describes a novel approach using an oligonucleotide chip to determine photosensitizer-induced DNA single-strand breaks. Surface coverage of fluorophore-labeled oligonucleotides on silicon dioxide chip surfaces was determined on alkaline phosphatase digestion. Fluorescence maxima (at 520 nm) of the solutions were converted to molar concentrations of the fluorescein-modified oligonucleotide by interpolation from a predetermined standard linear calibration curve. The photosensitizing activity of chlorpromazine and triflupromazine toward DNA single-strand breaks was then studied at different drug doses and also as a function of photoirradiation time. |
| 36 |
Direct Quantification of Photosensitizer Druginduced DNA Single Strand Breaks Using Oligonucleotide Chip
Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by Geni $ teiii" 4-0-?-L-rhainnopyranosyl-(l-2)-?-D-glucopyranoside through the NF-kB Inactivation in RAW 264.7 Cells |
| 37 |
Determination of Surface Coverage of Oligonucleotides Bound to Chip Surfaces by Alkaline Phosphatase Digestion
Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by Geni $ teiii" 4-0-?-L-rhainnopyranosyl-(l-2)-?-D-glucopyranoside through the NF-kB Inactivation in RAW 264.7 Cells |
| 38 |
Quantitation of ultraviolet-induced single-strand breaks using oligonucleotide chip
A simple, accurate and robust methodology was established for the direct quantification of ultraviolet (UV)-induced single-strand break (SSB) using oligonucleotide chip. Oligonucleotide chips were fabricated by covalently anchoring the fluorescent-labeled ssDNAs onto silicon dioxide chip surfaces. Assuming that the possibility of more than one UV-induced SSB to be generated in a small oligonucleotide is extremely low, SSB formation was investigated quantifying the endpoint probe density by fluorescence measurement upon UV irradiation. The SSB yields obtained based on the highly sensitive laser-induced fluorometric determination of fluorophore-labeled oligonucleotides were found to coincide well with that predicted from a theoretical extrapolation of the results obtained for plasmid DNAs using conventional agarose gel electrophoresis. |
| 39 |
Removal ofE. colifrom Water Using Surface-Modified Activated Carbon Filter Media and Its Performance over an Extended Use
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| 40 |
Parallel Applications of Integrated Circuit Bipolar Semiconductor Microchip as a Miniaturized Sample Platform and a Two-dimensional Photodetector for Optical Detection of Biomolecules
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| 41 |
Determination of Dose-depth Distribution of Proton Beam Using Resazurin Metabolism Assay
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| 42 |
Removal of Herbicides Using Surface Engineered Carbon
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| 43 |
Nanocrystalline-Silver Impregnated Activated Carbon Matrix with High Activity Antibacterial
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| 44 |
Electrochemiluninesce-based DNA Detection Using Microelectrode Array
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| 45 |
Microelectrode array for electrochemiluminescence-based DNA detection
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| 46 |
Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion
Silanized chip surfaces provide a reliable substrate for immobilization of oligonucleotides. The ability for rapid and sensitive detection of oligonucleotide surface coverage on these chips is crucial for their wide and effective applications in biotechnology. In this paper, two different silanization procedures were used to covalently bind fluorescent-labeled single-stranded DNA onto silicon dioxide or nitride chip surfaces. Effects of surface functionalization techniques for different surfaces, and immobilization conditions, including buffers and solution ionic strength, on surface probe coverage were investigated, quantifying the endpoint probe density by fluorescent measurement upon digestion with alkaline phosphatase (ALP). Digestion of surface-immobilized oligonuleotides with ALP released the fluorophore-tagged probe fragments back into the solution. |
| 47 |
Direct Evidence for Shape Dependent Antibacterial Activity of Silver Nanoparticles
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| 48 |
Single Nucleotide Polymorphism (SNP) Analysis Based on Capillary Electroch romatography (CEC) using Colloidal Poly (N-isopropylacrylamide) Particles as Pseudostationary Phase
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| 49 |
Miniaturized DNA Chip Based on Bipolar Semiconductor Technology
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| 50 |
Integrated circuit bipolar semiconductor microchip-based optical detection of biomolecules: Parallel applications as a miniaturized sample platform and a two-dimensional photodetector
Recently, our laboratory developed a photodiode array (PDA) microchip based on bipolar semiconductor technology. A 12 x 12 micro array of photodiodes, an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single IC chip. Each photodiode had dimensions of 300 x 300 mu m(2) and the photodiode-to-photodiode distance was 100 gm. The chip was successfully applied to the on-chip detection of target DNA and protein based on optical transduction detection method. This report demonstrates the compatibility of the constructed PDA chips to the on-chip reaction conditions and the effects of operational parameters that may lead to false data interpretation. The chip has also been used as a two dimensional photodetector in a compact optical system to detect Escherichia coli O157: H7 based on the combined use of ELISA and laser-induced fluorescence (LIF). |
| 51 |
Determination of the dose
In this study the dose?depth distribution pattern of proton beams was investigated by inactivation of human cells exposed to high-LET (linear energy transfer) protons. The proton beams accelerated up to 45 MeV were horizontally extracted from the cyclotron, and were delivered to the cells acutely through a home made prototype over a range of physical depths (in the form of a variable water column). The biological systems used here were two in vitro cell lines, including human embryonic kidney cells (HEK 293), and human breast adenocarcinoma cell line (MCF-7). Cells were exposed to unmodulated proton beam radiation at a dose of 50 Gy similar to that used in therapy. Resazurin metabolism assay was investigated for measurement of cell response to irradiation as a simple and non-destructive assay. |
| 52 |
Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli
In this work we investigated the antibacterial properties of differently shaped silver nanoparticles against the gram-negative bacterium Escherichia coli, both in liquid systems and on agar plates. Energy-filtering transmission electron microscopy images revealed considerable changes in the cell membranes upon treatment, resulting in cell death. Truncated triangular silver nanoplates with a {111} lattice plane as the basal plane displayed the strongest biocidal action, compared with spherical and rod-shaped nanoparticles and with Ag+ (in the form of AgNO3). It is proposed that nanoscale size and the presence of a {111} plane combine to promote this biocidal property. |
| 53 |
RETRACTED: Adsorption of cyanuric acid on activated carbon from aqueous solution: Effect of carbon surface modification and thermodynamic characteristics
The performance of a conventional and two surface-modified activated carbon samples was investigated for the sorption of cyanuric acid from aqueous solution by varying the process parameters such as initial concentration, pH, temperature, dose of adsorbents, and agitation time. The modified carbon samples obtained by acid and ammonia treatment of the conventional sample had a higher BET surface area (989 and 1010 m2?g?1, respectively) and higher specific mesopore surface area (27.36 and 33.21 m2?g?1, respectively) compared to the untreated material (820 and 18.25 m2?g?1). The solute removal was found to be favored at lower solute concentration, increased agitation time, increased adsorbent dose, and lower temperature. The modified adsorbents outperformed the conventional activated carbon for sorption of cyanuric acid especially at higher pH (>7.5) and with increasing temperature. |
| 54 |
Removal of E. coli from Water Using Surface-Modified Activated Carbon Filter Media and Its Performance over an Extended Use
Modification of activated carbon (AC) by aluminum hydroxychloride (AHC), and diatomaceous earth by zinc hydroxide changed the zeta potentials of these filter media from negative to positive. The modification method is amenable to room temperature, and eliminates the essential requirement of strong base treatment for making metal hydroxide coated filter media. Solid-state MAS 27Al NMR spectra suggested the presence of Al13-mer in the AHC-treated AC. AHC-modified AC samples were further treated with silver halide, and two antibacterial compounds to prevent microbial growth on filter media. In situ precipitation of silver bromide on AC resulted in formation of nanosized AgBr crystals. Bacteria removal performances of the modified media were tested in columns. |
| 55 |
Kyeong-Hee lee, Jong-Uk Kim, et al. Adsorption of cyanuric acid on activated carbon from aqueous solution: Effect of carbon surface modification and thermodynamic characteristics
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| 56 |
Oxovanadium (IV, V) complexes of salicylidene-2-picoloyl hydrazone Schiff base and related ligands
Oxovanadium (iv, v) complexes having O 4 N coordination environment have been synthesized in the solid state, formulated as M [VO 2 (L/L',/L")(H 2 O)], where M= K or NH 4; H 2 (L/L'/L") is the Schiff base ligand, obtained by condensation of salicylaldehyde with 2-picoloyl (L), 2-quinoloyl (L'), 8-quinoloyl (L") hydrazides. Dimeric complexes having the formulation [(VOL) 2 ?-O] have also been synthesized. Both the salicylidene-picoloyl hydrazone and the salicylidene-2-quinoloyl hydrazone ligands, in their dideprotonated form, coordinate to both vanadium in its+ 4 and+ 5 slate, through 0, N, O donor atoms, while, the ligand H 2 L" will act as a NNO donor ligand. Thus, in the dioxo vanadium complexes with the ligands L and L', the central vanadium core will have the O 4 N environment, which can be treated as a model to mimic the active site of vanadium haloperoxidases. |
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Single Nucleotide Polymorphism (SNP) Detection In Microfluidic Device Using Poly (NIsopropyDAcrylamide As Pseudostationary Phase
Akt-G SK 3p pathway underlies the cardioprotective effect of KR-32568, a novel sodium/hydrogen exchange r-1 inhibitor, in an isolated rat heart m odel of ischem iaand reperfusion heartinjury |
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Single pathogenic bacteria detection using a compact CMOS biochip system
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A model study for vanadium protein interactions: synthesis, characterization of oxovanadium (IV and V) complexes with l-carnosine
The potential metal binding sites of L-carnosine (beta-alanyl-L-histidine) with respect to vanadium has been investigated. Two novel oxovanadium complexes containing the dideprotonated L-carnosine anion were isolated having the composition NH4[VO2L] center dot 2H(2)O and [VOL] center dot 2H(2)O, where H2L = L-carnosine. The dideprotonated L-carnosine coordinates to vanadium in both the complexes through the three donor atoms, namely the oxygen atom of the carboxylate group and two nitrogen ( one each from the primary amine and the other from the amide group of the ligand). The imidazole moiety of L-carnosine undergoes a tautomeric change from N(3)-H to N(1)-H in the complex NH4[VO2L] center dot 2H(2)O. |
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