- 23 December, 2024
- Contact Us
- Webmail
- New Mail
- Skip To Main Content
- Screen Reader Access
- ONECSIR
A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. It is suitable for quantitative analyses. Generally, realtime PCR is based on the detection of the fluorescence produced by a reporter molecule that binds to the double stranded DNA which increases as the reaction proceeds.
