|
Novel application of Bacteriophage for controlling foaming and bulking in wastewater treatment plant (GAP-2128), (Year : 2017)
|
This addendum to "Novel application of bacteriophage for controlling foaming in wastewater treatment plant- an eco-friendly approach " includes characteristics of the phages NOC1, NOC2 and NOC3 not discussed in the previous paper. The phage adsorption and host interaction properties, their sensitivity to pH and temperature are inferred. NOC2 is seen to be more temperature resistant while others are not. All the phages show pH sensitivity. There is a variance observed in the behavior of these phages. Also, applicability of the phage based system to large scale reactors is studied and discussed here.
|
|
"Effects of UV radiations generated by VUV/UV excimer source on bacterial disinfection in water treatment. CSIR-NEERI and CSIR-CEERI PSC0101 ", (Year : 2017)
|
A structurally simple dielectric barrier discharge based mercury-free plasma UV-light source has been developed for efficient water disinfection. The source comprises of a dielectric barrier discharge arrangement between two co-axial quartz tubes with an optimized gas gap. The outer electrode is an aluminium baked foil tape arranged in a helical form with optimized pitch, while the inner electrode is a hollow aluminium metallic rod, hermetically sealed. Strong bands peaking at wavelengths 172 nm and 253 nm, along with a weak band peaking at wavelength 265 nm have been simultaneously observed due to plasma radiation from the admixture of xenon and iodine gases. The developed UV source has been used for bacterial deactivation studies using an experimental setup that is an equivalent of the conventional house-hold water purifier system. Deactivation studies for five types of bacteria, i.e., E. coli, Shigella boydii, Vibrio, Coliforms and Fecal coliform have been demonstrated with 4 log reductions in less than ten seconds
|
|
Application of bacteriophage for treating multidrug resistant (MDR) Pseudomonas aeruginosa (GAP-01-2066) , (Year : 2017)
|
"The main goal of this study was to evaluate the efficiency of phage therapy against one of the most common multidrug-resistant (MDR) agents of skin infections, Pseudomonas aeruginosa. The bacteriophage therapy is an effective antimicrobial approach with potentially important applications in medicine and biotechnology which can be seen as an additional string in the bow. Emerging drug resistant bacteria in aquaculture industry due to unrestricted use of antibiotics warrants more sustainable and environmental friendly strategies for controlling fish infections.The isolated bacteria from fish lesions was characterised based on isolation on selective and differential medium like Pseudomonas agar, gram staining, biochemical tests and 16SrRNA sequencing. The metallo-beta-lactamase (MBL) producing bacterial isolate was evaluated using Imipenem - Ethylenediaminetetraacetic acid (EDTA) disk method. The specific bacteriophage was isolated and concentrated using coal bed developed in our lab at CSIR-NEERI. The isolated and enriched bacteriophage was characterised by nucleotide sequencing and electron microscopy. The phage therapy was applied for treating ulcerative lesion in fish. RESULTS:The pathogenic bacterium responsible for causing ulcerative lesions in catfish species (Clarias gariepinus) was identified as Pseudomonas aeruginosa. One out of twenty P. aeruginosa isolate showing multi drug resistance (MDR) was incidentally found to be MBL producing as determined by Imipenem-EDTA disk method. The phage therapy effectively cured the ulcerative lesions of the infected fish in 8-10 days of treatment, with a sevenfold reduction of the lesion with untreated infection control. "
|
|
Assessment of water quality & sediment analysis to study the special property of river Ganga. , (Year : 2017)
|
Ganges, for centuries has been worshipped for its purity and healing powers. E. Hanbury Hankin, Felix D’Herelle and other scientists had done path-breaking research on Ganges river water, which lead to discovery of Bacteriophages. The National Mission for Clean Ganga awarded a special project to CSIR-NEERI to investigate deeper into the special properties of river Ganges. Researchers at CSIR-NEERI conducted a detailed and comprehensive study on Ganges to understand its special properties. The study has some unique featiures like the first study covering the entire span of river from Gomukh to Gangasagar; Bacteriophages against major water borne pathogenic bacterial groups of Escherichia, Enterobacter, Salmonella, Shigella, Vibrio being investigated in the study; Comparative study with control rivers Yamuna and Narmada to elucidate what’s special with Ganges. A massive sampling drive and study was conducted in pre and post monsoon seasons. The study on bacteriophages was done alongside physico-chemical properties, oxygen demand parameters, metal contents, flora, medicinal property etc of the Ganges river ecosystem by CSIR-NEERI and their association with special properties.
Host isolation and identification from all water and sediment samples of Ganga. Isolation and phage diversity study of bacteriophages in Ganga River Comparative study of Ganga River with Yamuna and Narmada River for phages diversity
|
|
Characterization of bacteriophage encoded lytic enzyme., (Year : 2017)
|
Use of bacteriophages as biocontrol agents is a promising tool for controlling pathogenic bacteria including antibiotic-resistant bacteria. Not only bacteriophages but also endolysins, the peptidoglycan hydrolyzing enzymes encoded by bacteriophages, have high potential for applications as biocontrol agents against food-borne pathogens. In this study, phage encoaded product viz. endolysin and holin were suucesfully purified and characterised . These phage encoaded product can be considered a favourable candidate for a new antimicrobial agent to control bacterial load.
|
