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Optimization of medium for the production of subtilisin from Bacillus subtilis MTCC 441
(Navaneeth, S., Bhuvanesh, S., Bhaskar, V., Vijay Kumar, P., Kandaswamy S.K.J., Anant,8,,6327-6331,Year : 2009)
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Subtilisins (E.C.3.4.21.62) are alkaline proteases that are secreted by members of the genus Bacillus. They are serine proteases that exhibit high specific activity on proteinaceous substrates, function optimally at moderate temperatures, and are stable under alkaline conditions. Thus the use of subtilisin as an enzyme additive could help in development of quality laundry detergents. In this work the subtilisin production from Bacillus subtilis (MTCC 441) was improved by altering and optimizing the media components. This alteration was brought up by process development strategy. The effect of yeast extract, casein, peptone and sodium chloride on subtilisin production was studied and were optimized using Box Behnken Design. The optimal growth conditions for B. subtilis MTCC 441 were found to 37 o C, and pH 7.5. The optimal media composition for subtilisin production was found to be yeast extract at 6.75 g/L, peptone at 4.41 g/L, sodium chloride at 6.08 g/L, casein at 10.75 g/L with glucose at 5 g/L. The predicted and observed response were 181.00 U/mg (with desirability =0.87) and 185.70 U/mg, respectively.
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Monitoring Photosensitizer Drug-induced DNA Single Strand Breaks Using Oligonucleotide Chip
(,Pages(252-252),,,Year : 2008)
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This paper describes a novel approach utilizing oligonucleotide chip for determining the photosensitizer-induced DNA single strand breaks (SSBs). Oligonucleotides chips were prepared by immobilizing fluorophore-labeled oligomers on to silicon oxide surfaces. Oligonucleotide surface coverage was determined by alkaline phosphatase (ALP) digestion method, wherein ALP (at high pH; 9.9) led to the cleavage of phosphates in the oligonucleotide backbone, releasing the fluorophore-tagged fragments back into the solution. The fluorescence maximums (measured at 520 nm) of the solutions containing those cleaved fragments were converted to molar concentrations of the fluorescein-modified oligonucleotide by interpolation from a predetermined standard linear calibration curve.
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Monitoring the (photo) genotoxicity of photosensitizer drugs: Direct quantitation of single-strand breaks in deoxyribonucleic acid using an oligonucleotide chip(Analytical biochemistry
)
(Min Jung Kim, Sukdeb Pal, Pravin K Naoghare, Joon Myong Song,Vol(382),Issue(1),Pages(40-47),,,Year : 2008)
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Oligonucleotide chip-based assays can be a sample-thrifty, time-saving, routine tool for evaluation of chemical-induced DNA strand breaks. This article describes a novel approach using an oligonucleotide chip to determine photosensitizer-induced DNA single-strand breaks. Surface coverage of fluorophore-labeled oligonucleotides on silicon dioxide chip surfaces was determined on alkaline phosphatase digestion. Fluorescence maxima (at 520 nm) of the solutions were converted to molar concentrations of the fluorescein-modified oligonucleotide by interpolation from a predetermined standard linear calibration curve. The photosensitizing activity of chlorpromazine and triflupromazine toward DNA single-strand breaks was then studied at different drug doses and also as a function of photoirradiation time.
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Direct Quantification of Photosensitizer Druginduced DNA Single Strand Breaks Using Oligonucleotide Chip
(KIM Min Jung, PAL Sukdeb, SONG Joon Myong,Pages(223-223),,,Year : 2008)
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Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by Geni $ teiii" 4-0-?-L-rhainnopyranosyl-(l-2)-?-D-glucopyranoside through the NF-kB Inactivation in RAW 264.7 Cells
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Determination of Surface Coverage of Oligonucleotides Bound to Chip Surfaces by Alkaline Phosphatase Digestion
(PAL Sukdeb, KIM Min Jung, SONG Joon Myong,Pages(301-301),,,Year : 2008)
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Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by Geni $ teiii" 4-0-?-L-rhainnopyranosyl-(l-2)-?-D-glucopyranoside through the NF-kB Inactivation in RAW 264.7 Cells
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Quantitation of ultraviolet-induced single-strand breaks using oligonucleotide chip(Analytica chimica acta
)
(Sukdeb Pal, Min Jung Kim, Jaebum Choo, Seong Ho Kang, Kyeong-Hee Lee, Joon Myong Song,Vol(622),Issue(1-2),Pages(195-200),,,Year : 2008)
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A simple, accurate and robust methodology was established for the direct quantification of ultraviolet (UV)-induced single-strand break (SSB) using oligonucleotide chip. Oligonucleotide chips were fabricated by covalently anchoring the fluorescent-labeled ssDNAs onto silicon dioxide chip surfaces. Assuming that the possibility of more than one UV-induced SSB to be generated in a small oligonucleotide is extremely low, SSB formation was investigated quantifying the endpoint probe density by fluorescence measurement upon UV irradiation. The SSB yields obtained based on the highly sensitive laser-induced fluorometric determination of fluorophore-labeled oligonucleotides were found to coincide well with that predicted from a theoretical extrapolation of the results obtained for plasmid DNAs using conventional agarose gel electrophoresis.
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Removal ofE. colifrom Water Using Surface-Modified Activated Carbon Filter Media and Its Performance over an Extended Use(Environmental Science and Technology
)
(Sukdeb Pal, J Joardar, Joon Myong Song
,Vol(42),Issue(10),Pages(3906-3906),,,Year : 2008)
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No information is available
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Parallel Applications of Integrated Circuit Bipolar Semiconductor Microchip as a Miniaturized Sample Platform and a Two-dimensional Photodetector for Optical Detection of Biomolecules
(TAK Yu Kyung, PAL Sukdeb, SONG Joon Myong,Vol(8),Pages(318-318),,,Year : 2008)
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No information is available
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Determination of Dose-depth Distribution of Proton Beam Using Resazurin Metabolism Assay
(KIM Min Jung, PAL Sukdeb, SONG Joon Myong
,Vol(8),Pages(317-317),,,Year : 2008)
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No information is available
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Removal of Herbicides Using Surface Engineered Carbon
(LEE Su Chul, PAL Sukdeb, SONG Joon Myong,Vol(8),Pages(317-317),,,Year : 2008)
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No information is available
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Nanocrystalline-Silver Impregnated Activated Carbon Matrix with High Activity Antibacterial
(PAL Sukdeb, SONG Joon Myong
,Vol(8),Pages(206-206),,,Year : 2008)
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No information is available
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Electrochemiluninesce-based DNA Detection Using Microelectrode Array
(PAL Sukdeb, SONG Joon Myong,Vol(8)Pages(312-312),,,Year : 2008)
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No information is available
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Microelectrode array for electrochemiluminescence-based DNA detection(Biochip Journal)
(Sukdeb Pal, Min Jung Kim, Joon Myong Song,Vol(2),Issue(1),Pages(66-72),,,Year : 2008)
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No information is available
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Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion(Lab on a Chip)
(Sukdeb Pal, Min Jung Kim, Joon Myong Song,Vol(8),Issue(8),Pages(1332-1341),,,Year : 2008)
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Silanized chip surfaces provide a reliable substrate for immobilization of oligonucleotides. The ability for rapid and sensitive detection of oligonucleotide surface coverage on these chips is crucial for their wide and effective applications in biotechnology. In this paper, two different silanization procedures were used to covalently bind fluorescent-labeled single-stranded DNA onto silicon dioxide or nitride chip surfaces. Effects of surface functionalization techniques for different surfaces, and immobilization conditions, including buffers and solution ionic strength, on surface probe coverage were investigated, quantifying the endpoint probe density by fluorescent measurement upon digestion with alkaline phosphatase (ALP). Digestion of surface-immobilized oligonuleotides with ALP released the fluorophore-tagged probe fragments back into the solution.
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Correlating on- line monitoring parameters, pH, DO and ORP with nutrient removal in an intermittent cyclic process bioreactor system(Bio-Resource Technology)
(Pankaj Tanwar, Tapas Nandy, Pallavi Ukey, Pravin Manekar,99,16,7630-7635,Year : 2008)
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The paper presents the study correlating the profile of on-line monitoring parameters and nutrient removal in an intermittent cyclic process bioreactor (ICPBR) system, thereby utilizing the parameters as operational tool. A laboratory scale ICPBR was employed to treat low C/N ratio domestic wastewater from a township. The study was conducted for correlating biological nutrient removal and on-line monitored parameters pH, dissolved oxygen (DO) and oxidation–reduction potential (ORP). The results revealed that pH, DO and ORP related with the dynamic behavior of nutrient concentration (NH4–N, NO3–N, and PO4–P) during treatment in an ICPBR system. The variation in pH and ORP of the reactor liquor correlate to conversion of ammonia (NH4–N) and nitrate (NO3–N) concentrations, respectively. As the bioconversion of ammonia nitrogen and phosphorus are related to the varying profile of the on-line monitored parameters, the profiles could possibly be used as onsite process control parameters.
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Water conservation through implementation of ultrafiltration and reverse osmosis system with recourse to recycling of effluent in textile industry(Resources, Conservation and Recycling)
(Tapas Nandy, Pravin Manekar, Rita Dhodapkar, Girish Pophali, Sukumar Devotta,Vol(51) Issue(1) Pages(64-77),,,Year : 2007)
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In compliance with the protection of the drinking water source in one of the state capital of India, a cluster of small scale textile industries implemented membrane processes based on treatability studies for recovery of boiler feed water quality with recourse to effluent recycling/reuse. The paper addresses to a case study in one of the textile units on upgradation of a full scale effluent treatment plant comprising chemical, biological, tertiary and advanced treatment processes. Based on the adequacy assessment of chemical and biological processes, improvement in the performance of the unit processes were achieved through optimization of coagulant dosage for chemical coagulation and build-up of active biomass in the activated sludge system. In addition, application of membrane separation processes comprising ultrafiltration and reverse osmosis units are also highlighted including disposal of reverse osmosis (RO) rejects through evaporator leading to zero liquid effluent discharge. The treatment scheme implemented resulted in conservation of around 55% of fresh water demand for industry.
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Direct Evidence for Shape Dependent Antibacterial Activity of Silver Nanoparticles
(TAK YU KYUNG, PAL SUKDEB, SONG JOON MYONG,Pages(332-332),,,Year : 2007)
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No information is available
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Single Nucleotide Polymorphism (SNP) Analysis Based on Capillary Electroch romatography (CEC) using Colloidal Poly (N-isopropylacrylamide) Particles as Pseudostationary Phase
(PAL SUKDEB, SONG JOON MYONG,Pages(397-397),,,Year : 2007)
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No information is available
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Miniaturized DNA Chip Based on Bipolar Semiconductor Technology
(PAL SUKDEB, KIM MIN JUNG, SONG JOON MYONG,Pages(399-399),,,Year : 2007)
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No information is available
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Integrated circuit bipolar semiconductor microchip-based optical detection of biomolecules: Parallel applications as a miniaturized sample platform and a two-dimensional photodetector(Biochip Journal)
(Sukdeb Pal, Joon Myong Song,Vol(1),Issue(2),Pages(124-133),,,Year : 2007)
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Recently, our laboratory developed a photodiode array (PDA) microchip based on bipolar semiconductor technology. A 12 x 12 micro array of photodiodes, an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single IC chip. Each photodiode had dimensions of 300 x 300 mu m(2) and the photodiode-to-photodiode distance was 100 gm. The chip was successfully applied to the on-chip detection of target DNA and protein based on optical transduction detection method. This report demonstrates the compatibility of the constructed PDA chips to the on-chip reaction conditions and the effects of operational parameters that may lead to false data interpretation. The chip has also been used as a two dimensional photodetector in a compact optical system to detect Escherichia coli O157: H7 based on the combined use of ELISA and laser-induced fluorescence (LIF).
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