Dr. Anshuman A. KHARDENAVIS

Designation:

Sr. Principal Scientist

Email Id:

aa_khardenavis[at]neeri[dot]res[dot]in

Qualification:

Ph.D.(Micro)

Specialization:

Microbiology

Address:

Sub-Vertical 2: Environmental Bioprocesses NEERI, Nagpur

Biodata:

View Biodata [0.12 MB]

Division

Sub-Vertical 2B : Environmental Bioprocesses

Sr. No.	Publication Name
1	In silico characterization of broad range proteases produced by Serratia marcescens EGD‐HP20 In the present study, Serratia marcescens EGD?HP20 strain was demonstrated to utilize poultry waste comprising of both white non?melanized and dark/brown melanized poultry feathers. The potential of the isolate to hydrolyze diverse keratinous wastes was further corroborated by comparative genomics which indicated the presence of genes for broad substrate specific proteases including metallo?proteases, serine endoprotease, dipeptidase, oligopeptidase, etc. Multiple gene sequence alignments of above genes showed 99–100% sequence identities with that of closely related strains of S. marcescens. The secondary structure, 3D structures and energy models suggested the stable nature of all the observed enzymes. Comparative genomics and hydrolysis of mixed feather waste indicated that the above potential of the isolate was associated with synergistic action of various types of proteases.
2	Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes In this study, we have characterized a novel set of extracellular enzymes produced by Penicillium chrysogenum strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with Penicillium rubens, Penicillium chrysogenum followed by Penicillium expansum and Penicillium roqueforti. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.

In silico characterization of broad range proteases produced by Serratia marcescens EGD‐HP20
In the present study, Serratia marcescens EGD?HP20 strain was demonstrated to utilize poultry waste comprising of both white non?melanized and dark/brown melanized poultry feathers. The potential of the isolate to hydrolyze diverse keratinous wastes was further corroborated by comparative genomics which indicated the presence of genes for broad substrate specific proteases including metallo?proteases, serine endoprotease, dipeptidase, oligopeptidase, etc. Multiple gene sequence alignments of above genes showed 99–100% sequence identities with that of closely related strains of S. marcescens. The secondary structure, 3D structures and energy models suggested the stable nature of all the observed enzymes. Comparative genomics and hydrolysis of mixed feather waste indicated that the above potential of the isolate was associated with synergistic action of various types of proteases.

Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes
In this study, we have characterized a novel set of extracellular enzymes produced by Penicillium chrysogenum strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with Penicillium rubens, Penicillium chrysogenum followed by Penicillium expansum and Penicillium roqueforti. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.

Last modified date : 01-08-2024

CSIR-NATIONAL ENVIRONMENTAL ENGINEERING RESEARCH INSTITUTE
A constituent laboratory of CSIR

Heading

Staff

Dr. Anshuman A. KHARDENAVIS

Staff ID

Division

Contact Number Extension

Publication

Heading

Heading