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An IMEA-PDA microchip for on-chip electrochemiluminescence detection
(Ji Yoen Kim, Sukdeb Pal, Min Jung Kim, Yu Kyung Tak, Ho Taik Kwon, Joon Myon Song,Pages(254-254),,,Year : 2009)
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This paper reports the design, fabrication and testing of a microchip wherein interdigitated microelectrode arrays (IMEA) were integrated with bipolar semiconductor photodiode array (PDA) chip to fabricate a highly compact embodiment for on-chip handling of solutions and electrochemiluminescence (ECL) detection. A 12? 12 micro array of photodiodes, each coupled withan interdigitated microelectrode array (IMEA), an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single 2? 2 cm2IC chip. Each photodiode had dimensions of 300? 300?m2 and the photodiode-to-photodiode distance was 100?m2. The chip was successfully applied to the on-chip quantification of electro chemiluminescing probelabeled single stranded oligonucleotides.
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Identification of ?-ray irradiated medicinal herbs by pulsed photostimulated luminescence, thermoluminescence, and electron spin resonance spectroscopy
(Won-Young Kim, Sukdeb Pal, Byeong Keun Kim, Min Jung Kim, Hyeon A Ki, Kyeong-Hee Lee, Woo Suk Kang, In Ho Kang, Shin Jung Kang, Joon Myon Song,Pages(253-253),,,Year : 2009)
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Dried herbal samples consisting of root, rhizome, cortex, fruit, peel, flower, spike, ramulus, folium, and whole plant of 20 different medicinal herbs were investigated using pulsed photostimulated luminescence (PPSL), thermoluminescence (TL), and electron spin resonance spectroscopy (ESR) to identify ?-ray irradiation treatment. Samples were irradiated at 0-59 kGy using a 60Co irradiator. PPSL measurement was applied as a rapid screening method. control samples of 19 different herbs had photon counts less than the lower and upper threshold values (700counts 60s-1). The photon counts of non-irradiated clematidis radix radix and irradiated evodia and gardenia fruits were between the lower and upper threshold values (700-5,000 counts 60s-1).
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Effects of ?-Ray Irradiation on Armeniacae Semen: A Three-Way Investigation Using GC-MS, HPLC, and Electron Spin Resonance
(KIM Min Jung, TAK Yu Kyung, KlM Won Young, PAL Sukdeb, KIM Byeong Keun, WOO Suk Kang, KANG InHo, KANG Shin Jung, SONG Joon Myong,Pages(226-226),,,Year : 2009)
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No information is available
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Identification of ?-Ray Irradiated Medicinal Herbs by Pulsed Photostimulated Luminescence, Thermoluminescence, and Electron Spin resonance Spectroscopy
(KlM WonYoung, PAL Sukdeb, KIM Min Jung, KI HyeonA, KIM Byeong Keun, KANG Woo Suk, LEE KyeongHee, KANG InHo, KANG Shin Jung, SONG Joon Myon,Pages(229-229),,,Year : 2009)
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Identification of ?-Ray Irradiated Medicinal Herbs by Pulsed Photostimulated Luminescence, Thermoluminescence, and Electron Spin resonance
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DNA Mutation Detection in Microfluidic Devices Using Poly (Nisopropyl) acrylamide As Pseudostationary Phase
(Yu Kyung Tak, Sukdeb Pal, Joon Myong Song,Pages(186-186),,,Year : 2009)
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Purpose: Recently, capillary electrochromatography (CEC) and mirochip electrochromatography (Microchip CEC) have gained attention due to the wide range of applications based on the use of open tubular capillaries. In this work, the use of phase-separated poly (N-isopropyl) acrylamide (PNIPAM) as a pseudostationary phase for CEC and microchip CEC is demonstrated for the detection of single nucleotide polymorphisms (SNPs). Methods: A typical cross channel microfluidic system was fabricated using an elastomeric material-poly (dimethylsiloxane)(PDMS). A master containing the network of the channels was created using photolithography. PDMS cast against the master yielded a polymeric replica. Conformal contact between the oxygen plasma oxidized surfaces of the PDMS replica and a flat PDMS slab formed the miniaturized capillary electrophoresis system
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Nanocrystalline silver supported on activated carbon matrix from hydrosol: antibacterial mechanism under prolonged incubation conditions(Journal of nanoscience and nanotechnology
)
(Sukdeb Pal, Yu Kyung Tak, J Joardar, Wook Kim, Jong Eun Lee, Myun Soo Han, Joon Myong Song,Vol(9),Issue(3),Pages(2092-2103),,,Year : 2009)
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Nanocrystalline silver-supported activated carbon (AC) was fabricated by directly loading silver nanoparticles into the porous AC matrix from a preformed nanosilver hydrosol. Silver-AC composites were also synthesized using a conventional thermal impregnation method. While XRD calculation indicated the presence of Ag crystallites in nanometer range, silver nanoparticle hydrosol-treated AC having the finest crystallite size CS (Escherichia coli. The kinetics of bacterial inactivation, in presence of hydroxyl radical (OH) scavengers, and superoxide anion radical (O? 2) inducer suggest the contribution of the reactive oxygen species (ROS) to antibacterial effect. However, these ROS scavengers did not show any inhibition of bactericidal activity after ?1 h, suggesting that generated ROS are responsible for E. coli inactivation only during the initial 1 h of the incubation time.
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Oligonucleotide chip assay for quantification of gamma ray-induced single strand breaks(Journal of pharmaceutical and biomedical analysis)
(Hyeon A Ki, Min Jung Kim, Sukdeb Pal, Joon Myong Song,Vol(49),Issue(2),Pages(562-566),,,Year : 2009)
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An oligonucleotide chip assay was designed for direct quantification of single strand breaks (SSBs) induced by ?-ray irradiation. The oligonucleotides used were 20-mers, which were short enough to produce only a single strand break within a single oligonucleotide. The two ends of the oligonucleotides were labeled with fluorescein and biotin, respectively. The biotinylated ends of the oligonucleotides were immobilized on a silicon wafer chip treated with (3-aminopropyl)triethoxysilane (APTES), glutaraldehyde, and avidin. The DNA fragments cleaved by ?-ray irradiation were detected by a laser-induced fluorescence (LIF) detection system. The ?-ray-induced SSBs were quantified using a calibration curve (fluorescence intensity versus ?-ray dose) without the need for complicated mathematical calculation based on gel-based separation.
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Synthesis, characterization and comparative evaluation of antivacterial activity of diverse morphological silver nanoparticles and higher-valent silver metallopharmaceuticals
(Pal Sukdeb
,,,,Year : 2009)
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No information is available
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Monitoring Photosensitizer Drug-induced DNA Single Strand Breaks Using Oligonucleotide Chip
(,Pages(252-252),,,Year : 2008)
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This paper describes a novel approach utilizing oligonucleotide chip for determining the photosensitizer-induced DNA single strand breaks (SSBs). Oligonucleotides chips were prepared by immobilizing fluorophore-labeled oligomers on to silicon oxide surfaces. Oligonucleotide surface coverage was determined by alkaline phosphatase (ALP) digestion method, wherein ALP (at high pH; 9.9) led to the cleavage of phosphates in the oligonucleotide backbone, releasing the fluorophore-tagged fragments back into the solution. The fluorescence maximums (measured at 520 nm) of the solutions containing those cleaved fragments were converted to molar concentrations of the fluorescein-modified oligonucleotide by interpolation from a predetermined standard linear calibration curve.
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Monitoring the (photo) genotoxicity of photosensitizer drugs: Direct quantitation of single-strand breaks in deoxyribonucleic acid using an oligonucleotide chip(Analytical biochemistry
)
(Min Jung Kim, Sukdeb Pal, Pravin K Naoghare, Joon Myong Song,Vol(382),Issue(1),Pages(40-47),,,Year : 2008)
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Oligonucleotide chip-based assays can be a sample-thrifty, time-saving, routine tool for evaluation of chemical-induced DNA strand breaks. This article describes a novel approach using an oligonucleotide chip to determine photosensitizer-induced DNA single-strand breaks. Surface coverage of fluorophore-labeled oligonucleotides on silicon dioxide chip surfaces was determined on alkaline phosphatase digestion. Fluorescence maxima (at 520 nm) of the solutions were converted to molar concentrations of the fluorescein-modified oligonucleotide by interpolation from a predetermined standard linear calibration curve. The photosensitizing activity of chlorpromazine and triflupromazine toward DNA single-strand breaks was then studied at different drug doses and also as a function of photoirradiation time.
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Direct Quantification of Photosensitizer Druginduced DNA Single Strand Breaks Using Oligonucleotide Chip
(KIM Min Jung, PAL Sukdeb, SONG Joon Myong,Pages(223-223),,,Year : 2008)
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Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by Geni $ teiii" 4-0-?-L-rhainnopyranosyl-(l-2)-?-D-glucopyranoside through the NF-kB Inactivation in RAW 264.7 Cells
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Determination of Surface Coverage of Oligonucleotides Bound to Chip Surfaces by Alkaline Phosphatase Digestion
(PAL Sukdeb, KIM Min Jung, SONG Joon Myong,Pages(301-301),,,Year : 2008)
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Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by Geni $ teiii" 4-0-?-L-rhainnopyranosyl-(l-2)-?-D-glucopyranoside through the NF-kB Inactivation in RAW 264.7 Cells
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Quantitation of ultraviolet-induced single-strand breaks using oligonucleotide chip(Analytica chimica acta
)
(Sukdeb Pal, Min Jung Kim, Jaebum Choo, Seong Ho Kang, Kyeong-Hee Lee, Joon Myong Song,Vol(622),Issue(1-2),Pages(195-200),,,Year : 2008)
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A simple, accurate and robust methodology was established for the direct quantification of ultraviolet (UV)-induced single-strand break (SSB) using oligonucleotide chip. Oligonucleotide chips were fabricated by covalently anchoring the fluorescent-labeled ssDNAs onto silicon dioxide chip surfaces. Assuming that the possibility of more than one UV-induced SSB to be generated in a small oligonucleotide is extremely low, SSB formation was investigated quantifying the endpoint probe density by fluorescence measurement upon UV irradiation. The SSB yields obtained based on the highly sensitive laser-induced fluorometric determination of fluorophore-labeled oligonucleotides were found to coincide well with that predicted from a theoretical extrapolation of the results obtained for plasmid DNAs using conventional agarose gel electrophoresis.
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Removal ofE. colifrom Water Using Surface-Modified Activated Carbon Filter Media and Its Performance over an Extended Use(Environmental Science and Technology
)
(Sukdeb Pal, J Joardar, Joon Myong Song
,Vol(42),Issue(10),Pages(3906-3906),,,Year : 2008)
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No information is available
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Parallel Applications of Integrated Circuit Bipolar Semiconductor Microchip as a Miniaturized Sample Platform and a Two-dimensional Photodetector for Optical Detection of Biomolecules
(TAK Yu Kyung, PAL Sukdeb, SONG Joon Myong,Vol(8),Pages(318-318),,,Year : 2008)
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No information is available
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Determination of Dose-depth Distribution of Proton Beam Using Resazurin Metabolism Assay
(KIM Min Jung, PAL Sukdeb, SONG Joon Myong
,Vol(8),Pages(317-317),,,Year : 2008)
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No information is available
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Removal of Herbicides Using Surface Engineered Carbon
(LEE Su Chul, PAL Sukdeb, SONG Joon Myong,Vol(8),Pages(317-317),,,Year : 2008)
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No information is available
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Nanocrystalline-Silver Impregnated Activated Carbon Matrix with High Activity Antibacterial
(PAL Sukdeb, SONG Joon Myong
,Vol(8),Pages(206-206),,,Year : 2008)
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No information is available
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Electrochemiluninesce-based DNA Detection Using Microelectrode Array
(PAL Sukdeb, SONG Joon Myong,Vol(8)Pages(312-312),,,Year : 2008)
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No information is available
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Microelectrode array for electrochemiluminescence-based DNA detection(Biochip Journal)
(Sukdeb Pal, Min Jung Kim, Joon Myong Song,Vol(2),Issue(1),Pages(66-72),,,Year : 2008)
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No information is available
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