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Role of fluoride induced epigenetic alterations in the development of skeletal fluorosis(Ecotoxicol Environ Saf. )
(,,,,Year : 2019)
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Oxidative stress is reported to negatively affect osteoblast cells. Present study reports oxidative and inflammatory signatures in fluoride-exposed human osteosarcoma (HOS) cells, and their possible association with the genes involved in osteoblastic differentiation and bone development pathways. HOS cells were challenged with sublethal concentration (8 mg/L) of sodium fluoride for 30 days and analyzed for transcriptomic expression. In total, 2632 transcripts associated with several biological processes were found to be differentially expressed. Specifically, genes involved in oxidative stress, inflammation, osteoblastic differentiation, and bone development pathways were found to be significantly altered. Variation in expression of key genes involved in the abovementioned pathways was validated through qPCR. Expression of serum amyloid A1 protein, a key regulator of stress and inflammatory pathways, was validated through western blot analysis. This study provides evidence that chronic oxidative and inflammatory stress may be associated with the fluoride-induced impediment in osteoblast differentiation and bone development.
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Stabilized landfill leachate treatment using heterogeneous Fenton and electro-Fenton processes(Chemosphere)
(T. Sruthi, R. Gandhimathi, S. T. Rameesh, P. V. Nidheesh,210,,38-43,Year : 2018)
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In the present study, stabilized landfill leachate treatment by heterogeneous Fenton and electro-Fenton (EF) was carried out. Iron-manganese binary oxide loaded zeolite (IMZ) was used as a catalyst for generating hydroxyl radicals in the acidic medium. Heterogeneous Fenton process was capable of removing 88.6% COD from landfill leachate at the optimal conditions, while 87.5% COD removal was observed at optimal EF treatment conditions. Biodegradability of landfill leachate was increased significantly from 0.03 to 0.52 after Fenton treatment. The prepared heterogeneous catalyst was found reusable with a reduction in COD removal rate. Even though, both the processes are efficient for leachate treatment, the low catalyst dosage requirement in case of EF process justifies that it is more feasible than Fenton process.
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Removal of Organic Pollutants using Peroxicoagulation Process(Environmental Chemistry Letters)
(P.V. Nidheesh,,,,Year : 2018)
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Peroxicoagulation is an electrochemical advanced oxidation processes in which both ferrous ions and hydrogen peroxide are generated in the cell. Organic pollutants are thus removed by degradation and coagulation. The peroxicoagulation process is a combination of electro-Fenton and electrocoagulation processes. The peroxicoagulation process is very efficient for the removal of aniline and herbicides from water and for the treatment of landfill leachate and textile wastewaters. Under acidic conditions, electro-Fenton is the predominant removal means, whereas electrocoagulation is the main removal means under neutral and alkaline conditions. As a consequence, pH regulation to acidic conditions is essential for the mineralization of organic pollutants.
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Fly ash toxicity, emerging issues and possible implications for its exploitation in agriculture,Indian Scenario(Chemosphere)
(Hemlata P. Jambhulkar, Siratun Montaha S. Shaikh, M. Suresh Kumar,213,,333-344,Year : 2018)
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Fly ash is considered as an environmental hazard worldwide, since it generally contain organic pollutants, probable toxic metals like Se, As, B, V, Al, Pb, Hg, Cr and radionuclide's Uranium, Thorium. Although fly ash contains toxic substances, it also contains most of the oxides and trace elements. Presence of oxides contributes to its alkaline pH while trace elements provides nutrients for plant growth hence, it is suggested that it can be used in low concentration in agriculture sector as well as a soil conditioner as fly ash improves the physico-chemical and biological properties of contaminated soils. This article presents a review on causes of fly ash toxicities due to organic pollutants, heavy metals, radioactive elements and environmental issues related to its utilization and possibilities of fly ash exploitation in agriculture sector such as phytoremediation, bioremediation, reclamation of wasteland and forestry.
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Composite wastewater treatement by aerated electrocoagulation and modified peroxi-coagulation process(Chemosphere)
(Abhijeet Kumar, P.V. Nidheesh, M. Suresh Kumar,205,,587-593,Year : 2018)
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No information is available
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Fluoride-Induced Oxidative and Inflammatory Stress in Osteosarcoma Cells: Does It Affect Bone Development Pathway
(Deepa Gandhi, Pravin K. Naoghare, Amit Bafana, Krishnamurthi Kannan & Saravanadevi Sivanesan,,,103-111,Year : 2017)
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No information is available
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Global DNA methylation profiling of manganese-exposed human neuroblastoma SH-SY5Y cells reveals epigenetic alterations in Parkinson's disease-associated genes(Arch Toxicol)
(Prashant Tarale. Saravandevi Sivanesan, Atul P. Daiwile, Reinhard Stoger, Amit Bafana, Pravin K. Naoghare, Devendra Parmar, Tapan Chakrabarti, Krishnamurthi K.,91(7),,2629-2641,Year : 2017)
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Manganese (Mn) is an essential trace element required for optimal functioning of cellular biochemical pathways in the central nervous system. Elevated exposure to Mn through environmental and occupational exposure can cause neurotoxic effects resulting in manganism, a condition with clinical symptoms identical to idiopathic Parkinson's disease. Epigenetics is now recognized as a biological mechanism involved in the etiology of various diseases. Here, we investigated the role of DNA methylation alterations induced by chronic Mn (100 µM) exposure in human neuroblastoma (SH-SY5Y) cells in relevance to Parkinson's disease. A combined analysis of DNA methylation and gene expression data for Parkinson's disease-associated genes was carried out. Whole-genome bisulfite conversion and sequencing indicate epigenetic perturbation of key genes involved in biological processes associated with neuronal cell health. Integration of DNA methylation data with gene expression reveals epigenetic alterations to PINK1, PARK2 and TH genes that play critical roles in the onset of Parkinsonism. The present study suggests that Mn-induced alteration of DNA methylation of PINK1-PARK2 may influence mitochondrial function and promote Parkinsonism. Our findings provide a basis to further explore and validate the epigenetic basis of Mn-induced neurotoxicity.
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Chemical characterization of simulated landfill soil leachates from Nigeria and India and their cytotoxicity and DNA damage inductions on three human cell lines(Chemosphere)
(Alimba CG, Gandhi D, Sivanesan S, Bhanarkar MD, Naoghare PK, Bakare AA, Krishnamurthi K.,164,,469-479,Year : 2016)
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Landfill soils are sources of emerging carcinogens, teratogens and mutagens in the environment. There is inadequate information on its possible health risk and cytogenotoxicity. This study evaluated chemical characterization of four simulated landfill leachates with their cytotoxicity and DNA damage in human cells. Hepatocarcinoma (HepG2), lymphoma (Jurkat) and osteosarcoma (HOS) cells, incubated with 6.25, 12.5, 25, 50, 75 and 100% of Aba Eku (AEL), Olusosun (OSL), Awotan (AWL) and Nagpur (NPL) simulated leachates for 24 h, were assessed for cell viability using MTT assay and morphological alterations. DNA damage was also assessed after 24 h treatment of cells with sub-lethal concentrations of the leachates using comet assay. Metals and organic compounds in the soil leachates were determined using inductively coupled plasma-mass spectrometry (ICP-MS) and gas chromatography-mass spectroscopy (GC-MS) respectively. The leachates induced significant cytotoxicity in the treated cells with evidence of apoptosis; shrunken morphologies, detachment from the substratum and cytoplasmic vacuolations. Similarly, there was significant DNA damage induced in the treated cells, with increased Olive tail moment, tail length and % tail DNA. Jurkat was the most sensitive (Jurkat > HepG2 > HOS) to the cytotoxic and genotoxic effects of the leachates. All the analyzed metals except Cd, Fe, Zn and Mn were found at levels lower than standard allowable limits. 32, 17, 23 and 23 different PAHs and PCBs were detected in AEL, AWL, OSL and NPL respectively, at varying retention peak times. These toxic constituents induced the observed cytogenotoxicity in the cells and may suggest possible public health risk.
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Potential Role of Epigenetic Mechanism in Manganese Induced Neurotoxicity(Biomed. Res. International)
(Prashant Tarale, Tapan Chakrabarti, Saravanadevi Sivanesan, Pravin Naoghare, Amit Bafana, and Kannan Krishnamurthi,,,,Year : 2016)
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Manganese is a vital nutrient and is maintained at an optimal level (2.5–5 mg/day) in human body. Chronic exposure to manganese is associated with neurotoxicity and correlated with the development of various neurological disorders such as Parkinson’s disease. Oxidative stress mediated apoptotic cell death has been well established mechanism in manganese induced toxicity. Oxidative stress has a potential to alter the epigenetic mechanism of gene regulation. Epigenetic insight of manganese neurotoxicity in context of its correlation with the development of parkinsonism is poorly understood. Parkinson’s disease is characterized by the -synuclein aggregation in the form of Lewy bodies in neuronal cells. Recent findings illustrate that manganese can cause overexpression of -synuclein. -Synuclein acts epigenetically via interaction with histone proteins in regulating apoptosis. -Synuclein also causes global DNA hypomethylation through sequestration of DNA methyltransferase in cytoplasm. An individual genetic difference may also have an influence on epigenetic susceptibility to manganese neurotoxicity and the development of Parkinson’s disease. This review presents the current state of findings in relation to role of epigenetic mechanism in manganese induced neurotoxicity, with a special emphasis on the development of Parkinson’s disease.
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Integrative genomic and proteomic profiling of human neuroblastoma SH-SY5Y cells reveals signatures of endosulfan exposure(Environmental Toxicology and Pharmacology)
(Deepa Gandhi, Prashant Tarale, Pravin K Naoghare, Amit Bafana, Krishnamurthi Kannan, Saravanadevi Sivanesan,41,,187-194,Year : 2016)
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Endosulfan, an organochlorine pesticide, is known to induce multiple disorders/abnormalities including neuro-degenerative disorders in many animal species. However, the molecular mechanism of endosulfan induced neuronal alterations is still not well understood. In the present study, the effect of sub-lethal concentration of endosulfan (3 μM) on human neuroblastoma cells (SH-SY5Y) was investigated using genomic and proteomic approaches. Microarray and 2D-PAGE followed by MALDI-TOF-MS analysis revealed differential expression of 831 transcripts and 16 proteins in exposed cells. A gene ontology enrichment analysis revealed that the differentially expressed genes and proteins were involved in variety of cellular events such as neuronal developmental pathway, immune response, cell differentiation, apoptosis, transmission of nerve impulse, axonogenesis, etc. The present study attempted to explore the possible molecular mechanism of endosulfan induced neuronal alterations in SH-SY5Y cells using an integrated genomic and proteomic approach. Based on the gene and protein profile possible mechanisms underlying endosulfan neurotoxicity were predicted.
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Spatial and temporal assessment of groundwater quality in Puri city, India: a statistical analysis(Asian Journal of Water, Environment and Pollution)
(Ritesh Vijay, S S Ramya P. R. Pujari and P. K. Mohapatra,10,2,51-58,Year : 2013)
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Puri city is situated near Bay of Bengal where there is a balanced interface of sea water and fresh water near the coast line. The city receives water from the groundwater sources and the quality of ground water is being maintained. The main objective of the paper is to assess the quality of ground water using statistical techniques and the suitability of this water for its utility was verifi ed using Indian drinking water standards. Groundwater samples were collected from various specifi c areas during post and pre- monsoon seasons and analyzed for physico-chemical and bacteriological parameters and compared with standards. The ground water is mainly polluted due to the domestic effl uent, which contains detergents, human and animal excreta, industrial waste and cremation of human and animal bodies. The study showed that concentration of quality parameters were more in the city area as
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Geospatial assessment of water quality in creeks and coast of Mumbai India(International journal for scientific research in environemtal sciences )
(Ritesh Vijay, Puja Khobragade, S.R. Kamble, R. A. Sohony Rakesh Kumar ,4,12,61-67,Year : 2013)
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Mumbai, the financial capital of India is generating about 2700 MLD of sewage from seven service areas and discharging into adjoining coast, Malad, Mahim, Marve and Thane Creeks. The coastal and creeks water quality is deteriorating due to disposal of partially treated sewage, wastewater from open drains as well as industrial wastewater. The objective of present paper is to assess and evaluate the water quality during low and high tides. 65 samples from west coast including beaches and seafronts, 44 from creeks and 3 from ocean outfalls were collected as per Standard Method. The samples were analysed for physico-chemical and bacteriological parameters and results were compared with SW II standards as prescribed by Central Pollution Control Board, India. The results were incorporated on the GIS platform for further geospatial analysis and visualizati
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Feasibility of bioengineered two-stages sequential batch reactor and filtration - adsorption process for complex agrochemical effluent(Bioresource Technology)
(Pravin Manekar, Rima Biswas, Chaitali Urewar, Sukdeb Pal, Tapas Nandy,148,,334-342,Year : 2013)
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In the present study, the feasibility of a bioengineered two-stages sequential batch reactor (BTSSBR) followed by filtration–adsorption process was investigated to treat the agrochemical effluent by overcoming factor affecting process stability such as microbial imbalance and substrate sensitivity. An air stripper stripped 90% of toxic ammonia, and combined with other streams for bio-oxidation and filtration–adsorption. The BTSSBR system achieved bio-oxidation at 6 days hydraulic retention time by fending off microbial imbalance and substrate sensitivity. The maximum reduction in COD and BOD by heterotrophic bacteria in the first reactor was 87% and 90%, respectively. Removal of toxic ammoniacal-nitrogen by autotrophic bacteria in a post-second stage bio-oxidation was 97%. The optimum filtration and adsorption of pollutants were achieved at a filtration rate of 10 and 9 m3 m?2 h?1, respectively. The treatment scheme comprising air stripper, BTSSBR and filtration–adsorption process
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Statistical assessment of seafront and beach water quality of Mumbai, India(Water Science and Technology)
(Ritesh Vijay,S. Kamble, S. S. Dhage, R. A. Sohony, S. R. Wate ,64,5, 1186-1193,Year : 2011)
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The water quality of seafronts and beaches of Mumbai is under pressure and deteriorating due to discharge of partially treated sewage and wastewater through point and nonpoint sources. The objective of the study was to assess the water quality and to correlate physico-chemical and bacteriological parameters for establishing relationship, association and dependence on each other. The water quality parameters were selected as per SW II standards specified by Central Pollution Control Board, India and nutrient parameters as strong indicators of sewage pollution. Box and whisker plots were generated for evaluating spatio temporal variation of water quality which suggest influence of organic pollution mostly at Mahim and Dadar in the form of outliers and extremes. Pearson's correlations were estimated between parameters and found significant correlation with each other indicating influence of sewage on water quality. The water quality of beaches and seafronts were found unsafe for recreational purposes. Th
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Determination of processes affecting groundwater quality in the coastal aquifer beneath Puri City, India: a multivariate statistical approach(Water Science and Technology)
(P. K. Mohapatra, Ritesh Vijay, P. R. Pujari, S. K. Sundaray, B. P. Mohanty ,64,4,809 - 817,Year : 2011)
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Variability of groundwater quality parameters is linked to various processes such as weathering, organic matter degradation, aerobic respiration, iron reduction, mineral dissolution and precipitation, cation exchange and mixing of salt water with fresh water. Multivariate statistical analyses such as principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied to the standardized data set of eleven groundwater quality parameters (i.e. pH, Ca2+, Mg2+, Na+, K+, Fe3+, alkalinity, NO3-, Cl-, SO4(2-), TDS) collected during the post-monsoon and the summer seasons in order to elicit hydrologic and biogeochemical processes affecting water quality in the unconfined aquifer beneath Puri city in eastern India. The application of PCA resulted in four factors explaining 73% variance in post-monsoon and 81% variance in summer. The HCA using Ward's method and squared Euclidean distance measure classified the parameters into four clusters based on their similarities. PCA and HCA allowed interpretation of processes. During both post-monsoon and summer seasons, anthropogenic pollution and organic matter degradation/Fe(III) reduction were found dominant due to contribution from on-site sanitation in septic tanks and soak pits in the city. Cation exchange and mineral precipitation were possible causes for increase in Na+ and decrease in Ca2+ concentration in summer. Fresh water recharge during monsoon and Sea water intrusion in summer are attributed as significant hydrologic processes to variations of the groundwater quality at the study site.
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Spatio-temporal assessment of groundwater level and quality in urban coastal city Puri, India(Water Science and Technology: Water Supply)
(Ritesh Vijay, S. S. Ramya, P. R. Pujari, and P. K. Mohapatra,11,2,194-201,Year : 2011)
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Puri city is situated on the shoreline of Bay of Bengal and groundwater is the only source available to meet the city water supply mainly from two demarcated water fields. Due to the anthropogenic activities and absence of sewerage system, groundwater quality is deteriorating through open discharges of domestic waste, open defecation, septic tanks, soak pits, contaminated water pools, unorganized waste dumping etc. The objective of the paper was to assess seasonal groundwater fluctuation and water quality in terms of physcio-chemical and bacteriological parameters. For this, groundwater levels were measured and samples were collected during post-monsoon (November 2006) and pre-monsoon (June 2007) from the water fields and city area. Groundwater quality was
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Assessment of water quality using cluster analysis in coastal region of Mumbai, India(Environmental Monitoring and Assessment)
(Swapnil R Kamble Ritesh Vijay,178,1-17,321-332,Year : 2011)
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The coastal water quality of Mumbai is deteriorating due to various point and non-point wastewater sources. Hence, it is desirable to monitor coastal water quality for various water-related activities like bathing, contact water sports, recreation, and commercial fishing. The objective of this paper is to assess the seasonal water quality on the basis of seawater standards. Based on water-quality analysis of 17 seafronts and beaches, most of the parameters were exceeding the standards. The statistical cluster analysis was carried out for evaluating impact of wastewater and sewage discharges. The hierarchical cluster analysis resulted into three clustered groups, namely less polluted, moderately polluted, and highly polluted sites with similar characteristics of water quality. Mahim was found to be worst-affected beach due to incoming organi
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Assessment of groundwater quality in Puri city, India: An impact of anthropogenic activities(Environmental Monitoring and Assessment)
(Ritesh Vijay, Puja Khobragade P. K. Mohapatra,177,1-4,409-418,Year : 2011)
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Puri City is situated on the east coast of India and receives water supply only from the groundwater sources demarcated as water fields. The objective of this paper is to assess and evaluate the groundwater quality due to impact of anthropogenic activities in the city. Groundwater samples were collected from the water fields, hand pumps, open wells, and open water bodies during post-monsoon 2006 and summer 2007. Groundwater quality was evaluated with drinking water standards as prescribed by Bureau of Indian Standards and Environmental Protection Agency to assess the suitability. The study indicated seasonal variation of water-quality parameters within the water fields and city area. Groundwater in the water fiel
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Monitoring changes in land use land cover of Yamuna riverbed in Delhi: a multi-temporal analysis
(R. Joshi, M. Warthe, S. harad Dwivedi, Ritesh Vijay T. Chakrabarti,,,,Year : 2011)
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Impact of anthropogenic activities has led to significant changes in riverbeds over a period of time. The objective of the study was to monitor the land use land cover (LULC) of Yamuna riverbed in Delhi and to assess the changes due to natural and anthropogenic activities. The maximum likelihood classification was carried out by using March 1977, April 1999, April 2002 and February 2009 imageries. An overall accuracy of LULC classification of 2009 imagery was around 88.6% based on ground truth data. A significant reduction in the main river course and increase in agricultural activities as well as built up were observed in remote sensing analysis as an outcome
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Fluctuation of groundwater in an urban coastal city of India: a GIS based approach(Hydrological Processes)
(Ritesh Vijay, Abhinav Sharma, S. S. Ramya and Apurba Gupta,25,,1479-1485,Year : 2011)
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Groundwater is the most important and valuable natural resources especially in coastal urban environment where surface water is insufficient to satisfy the water requirement. Puri city is located on the east coast of India where groundwater is the only source available to meet city water supply. As the city is situated on the sandy aquifer, quality of groundwater is deteriorating because of anthropogenic activities, lack of sewerage system, etc. The objective of the study was to assess the groundwater fluctuation during post-monsoon and summer with respect to hydrogeological conditions, topography, and groundwater consumption pattern of the city. For this assessment and analysis, Geographic Information System (GIS) was used to visualize topography of the area through digital elevation model (DEM) and distribution of groundwater contours spatially and temporally. The probable areas prone to contamination were identified based on aquifer property and depths to water table below ground. Copyright © 2010 John Wiley & Sons, Ltd.
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