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Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion(Lab on a Chip)
(Sukdeb Pal, Min Jung Kim, Joon Myong Song,Vol(8),Issue(8),Pages(1332-1341),,,Year : 2008)
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Silanized chip surfaces provide a reliable substrate for immobilization of oligonucleotides. The ability for rapid and sensitive detection of oligonucleotide surface coverage on these chips is crucial for their wide and effective applications in biotechnology. In this paper, two different silanization procedures were used to covalently bind fluorescent-labeled single-stranded DNA onto silicon dioxide or nitride chip surfaces. Effects of surface functionalization techniques for different surfaces, and immobilization conditions, including buffers and solution ionic strength, on surface probe coverage were investigated, quantifying the endpoint probe density by fluorescent measurement upon digestion with alkaline phosphatase (ALP). Digestion of surface-immobilized oligonuleotides with ALP released the fluorophore-tagged probe fragments back into the solution.
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Direct Evidence for Shape Dependent Antibacterial Activity of Silver Nanoparticles
(TAK YU KYUNG, PAL SUKDEB, SONG JOON MYONG,Pages(332-332),,,Year : 2007)
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No information is available
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Single Nucleotide Polymorphism (SNP) Analysis Based on Capillary Electroch romatography (CEC) using Colloidal Poly (N-isopropylacrylamide) Particles as Pseudostationary Phase
(PAL SUKDEB, SONG JOON MYONG,Pages(397-397),,,Year : 2007)
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No information is available
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Miniaturized DNA Chip Based on Bipolar Semiconductor Technology
(PAL SUKDEB, KIM MIN JUNG, SONG JOON MYONG,Pages(399-399),,,Year : 2007)
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No information is available
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Low Noise Bipolar semiconductor Protein Chip for On-Chip Detection ofE. coliO157: H7
(KIM MIN JUNG, PAL SUKDEB, TAK YU KYUNG SONG JOON MYONG,,,,Year : 2007)
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No information is available
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Integrated circuit bipolar semiconductor microchip-based optical detection of biomolecules: Parallel applications as a miniaturized sample platform and a two-dimensional photodetector(Biochip Journal)
(Sukdeb Pal, Joon Myong Song,Vol(1),Issue(2),Pages(124-133),,,Year : 2007)
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Recently, our laboratory developed a photodiode array (PDA) microchip based on bipolar semiconductor technology. A 12 x 12 micro array of photodiodes, an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single IC chip. Each photodiode had dimensions of 300 x 300 mu m(2) and the photodiode-to-photodiode distance was 100 gm. The chip was successfully applied to the on-chip detection of target DNA and protein based on optical transduction detection method. This report demonstrates the compatibility of the constructed PDA chips to the on-chip reaction conditions and the effects of operational parameters that may lead to false data interpretation. The chip has also been used as a two dimensional photodetector in a compact optical system to detect Escherichia coli O157: H7 based on the combined use of ELISA and laser-induced fluorescence (LIF).
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Determination of the dose(Analytica chimica acta)
(Min Jung Kim, Sukdeb Pal, Yu Kyung Tak, Kyeong-Hee Lee, Tae Keun Yang, Su-Jae Lee, Joon Myong Song,Vol(593),Issue(2),Pages(214-223),,,Year : 2007)
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In this study the dose?depth distribution pattern of proton beams was investigated by inactivation of human cells exposed to high-LET (linear energy transfer) protons. The proton beams accelerated up to 45 MeV were horizontally extracted from the cyclotron, and were delivered to the cells acutely through a home made prototype over a range of physical depths (in the form of a variable water column). The biological systems used here were two in vitro cell lines, including human embryonic kidney cells (HEK 293), and human breast adenocarcinoma cell line (MCF-7). Cells were exposed to unmodulated proton beam radiation at a dose of 50 Gy similar to that used in therapy. Resazurin metabolism assay was investigated for measurement of cell response to irradiation as a simple and non-destructive assay.
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Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli(Applied and environmental microbiology)
(Sukdeb Pal, Yu Kyung Tak, Joon Myong Song,Vol(73),Issue(6),Pages(1712-1720),,,Year : 2007)
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In this work we investigated the antibacterial properties of differently shaped silver nanoparticles against the gram-negative bacterium Escherichia coli, both in liquid systems and on agar plates. Energy-filtering transmission electron microscopy images revealed considerable changes in the cell membranes upon treatment, resulting in cell death. Truncated triangular silver nanoplates with a {111} lattice plane as the basal plane displayed the strongest biocidal action, compared with spherical and rod-shaped nanoparticles and with Ag+ (in the form of AgNO3). It is proposed that nanoscale size and the presence of a {111} plane combine to promote this biocidal property.
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RETRACTED: Adsorption of cyanuric acid on activated carbon from aqueous solution: Effect of carbon surface modification and thermodynamic characteristics(Journal of colloid and interface science)
(Sukdeb Pal, Kyeong-Hee Lee, Jong-Uk Kim, Seung-Hee Han, Joon Myong Song,Vol(303),Issue(1),Pages(39-48),,,Year : 2007)
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The performance of a conventional and two surface-modified activated carbon samples was investigated for the sorption of cyanuric acid from aqueous solution by varying the process parameters such as initial concentration, pH, temperature, dose of adsorbents, and agitation time. The modified carbon samples obtained by acid and ammonia treatment of the conventional sample had a higher BET surface area (989 and 1010 m2?g?1, respectively) and higher specific mesopore surface area (27.36 and 33.21 m2?g?1, respectively) compared to the untreated material (820 and 18.25 m2?g?1). The solute removal was found to be favored at lower solute concentration, increased agitation time, increased adsorbent dose, and lower temperature. The modified adsorbents outperformed the conventional activated carbon for sorption of cyanuric acid especially at higher pH (>7.5) and with increasing temperature.
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Removal of E. coli from Water Using Surface-Modified Activated Carbon Filter Media and Its Performance over an Extended Use(Environmental science & technology)
(Sukdeb Pal, J Joardar, Joon Myong Song,Vol(40),Issue(19),Pages(6091-6097),,,Year : 2006)
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Modification of activated carbon (AC) by aluminum hydroxychloride (AHC), and diatomaceous earth by zinc hydroxide changed the zeta potentials of these filter media from negative to positive. The modification method is amenable to room temperature, and eliminates the essential requirement of strong base treatment for making metal hydroxide coated filter media. Solid-state MAS 27Al NMR spectra suggested the presence of Al13-mer in the AHC-treated AC. AHC-modified AC samples were further treated with silver halide, and two antibacterial compounds to prevent microbial growth on filter media. In situ precipitation of silver bromide on AC resulted in formation of nanosized AgBr crystals. Bacteria removal performances of the modified media were tested in columns.
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Kyeong-Hee lee, Jong-Uk Kim, et al. Adsorption of cyanuric acid on activated carbon from aqueous solution: Effect of carbon surface modification and thermodynamic characteristics(Journal of colloid and interface science)
(Sukdeb Pal
,,,,Year : 2006)
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No information is available
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Oxovanadium (IV, V) complexes of salicylidene-2-picoloyl hydrazone Schiff base and related ligands(Journal of the Indian Chemical Society)
(Asit R SARKAR, Shipra MANDAL, Sukdeb PAL, Somnath CHAKRABARTY, Aniruddha CHAKRABARTY,Vol(83),Issue(2),Pages(185-190),,,Year : 2006)
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Oxovanadium (iv, v) complexes having O 4 N coordination environment have been synthesized in the solid state, formulated as M [VO 2 (L/L',/L")(H 2 O)], where M= K or NH 4; H 2 (L/L'/L") is the Schiff base ligand, obtained by condensation of salicylaldehyde with 2-picoloyl (L), 2-quinoloyl (L'), 8-quinoloyl (L") hydrazides. Dimeric complexes having the formulation [(VOL) 2 ?-O] have also been synthesized. Both the salicylidene-picoloyl hydrazone and the salicylidene-2-quinoloyl hydrazone ligands, in their dideprotonated form, coordinate to both vanadium in its+ 4 and+ 5 slate, through 0, N, O donor atoms, while, the ligand H 2 L" will act as a NNO donor ligand. Thus, in the dioxo vanadium complexes with the ligands L and L', the central vanadium core will have the O 4 N environment, which can be treated as a model to mimic the active site of vanadium haloperoxidases.
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Single Nucleotide Polymorphism (SNP) Detection In Microfluidic Device Using Poly (NIsopropyDAcrylamide As Pseudostationary Phase
(PAL Sukdeb, SONG Joon Myong,Pages(489-489),,,Year : 2005)
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Akt-G SK 3p pathway underlies the cardioprotective effect of KR-32568, a novel sodium/hydrogen exchange r-1 inhibitor, in an isolated rat heart m odel of ischem iaand reperfusion heartinjury
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Single pathogenic bacteria detection using a compact CMOS biochip system
(PARK Ahyun, PAL Sukdeb, SONG Joon Myong,Pages(477-477),,,Year : 2005)
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No information is available
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A model study for vanadium protein interactions: synthesis, characterization of oxovanadium (IV and V) complexes with l-carnosine(SYNTHESIS AND REACTIVITY IN INORGANIC METAL-ORGANIC AND NANO-METAL CHEMISTRY)
(AR Sarkar, S Mandal, S Pal, S Chakrabarty, A Chakrabarty,Vol(35),Issue(2),Pages(127-132),,,Year : 2005)
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The potential metal binding sites of L-carnosine (beta-alanyl-L-histidine) with respect to vanadium has been investigated. Two novel oxovanadium complexes containing the dideprotonated L-carnosine anion were isolated having the composition NH4[VO2L] center dot 2H(2)O and [VOL] center dot 2H(2)O, where H2L = L-carnosine. The dideprotonated L-carnosine coordinates to vanadium in both the complexes through the three donor atoms, namely the oxygen atom of the carboxylate group and two nitrogen ( one each from the primary amine and the other from the amide group of the ligand). The imidazole moiety of L-carnosine undergoes a tautomeric change from N(3)-H to N(1)-H in the complex NH4[VO2L] center dot 2H(2)O.
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Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatography(J. Ind. Microbiol. Biotechnol.)
(Bhuvanesh, S., Arunkumar, C., Kaliraj, P., Ramalingam, S.,Vol(37) Pages(1053-1059),,,Year : 2010)
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Abundant larval transcript (ALT), a novel filarial protein, has been shown to have great potential as a vaccine in the prevention of human lymphatic filariasis. In this study, we report a method for the production of recombinant ALT-2 protein, expressed in the cytoplasm of bacterium Escherichia coli in soluble form and purification in a single step using hydrophobic interaction chromatography (HIC). Fermentation was done by continuous fed-batch methodology with dissolved oxygen (DO)-controlled feed addition. The culture was induced with 1 mM isopropyl-?-D-thiogalactopyranoside (IPTG). Up to 9 g/l dry cell weight (DCW) of biomass was obtained from 1.6 l of Luria?Bertani (LB) broth in a bench-scale reactor. Around 200 mg/l of purified ALT-2 with a yield of about 60% was obtained. This is almost a 2.5-fold increase in final protein yield compared to purification using immobilized metal affinity chromatography (IMAC).
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This is sample research paper(Chemical Engineering Journal)
(H.S.Jain,280,,391-398,Year : 2021)
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This is sample paragraph for abstract
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Molecular Mechanism of Apoptosis Induction in Jurkat E6-1 Cells by Tribulus Terrestris Alkaloids Extract(Traditional and Complementary Medicines)
(Shriniwas S. Basaiyye, Pravin K. Naoghare, Sanjeev Kanojiya, Amit Bafana, Patrizio Arrigo, Kannan Krishnamurthi, Saravanadevi Sivanesan,,,,Year : 2017)
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The present study demonstrates apoptosis-inducing potential and mechanism of action of Tribulus terristris alkaloid extract in Jurkat E6-1 cancer cell line. Liquid Chromatography-Mass Spectrometry and High Resolution-Mass Spectrometry analysis identified the presence of four N-feruloyltyramine derivatives, namely trans-N-feruloyl-3-hydroxytyramine (1), trans-N-coumaroyltyramine (2), trans-N-feruloyltyramine (3) and trans-N-feruloyl-3-ethoxytyramine (4) in the alkaloid extract. Compounds 2 and 3 have not been yet reported in the alkaloid extract of T. terristris. In silico analysis revealed therapeutic potential of N-feruloyltyramine derivatives and strong binding efficiency to both chains of Tumor Necrosis Factor Receptor 1. Treatment of alkaloids extract to Jurkat E6-1 clone induced dose-dependent cytotoxicity (LC50 140.4 μg mL−1). Jurkat cells treated with alkaloids extract at sub-lethal concentration showed DNA fragmentation, enhancement in caspase-3 activity and phosphatidylserine translocation (apoptosis indicator) compared to control cells. Gene expression analysis using Human Apoptosis RT2 Profiler PCR Array analysis upon alkaloid treatment was found to significantly alter expression of critical genes such as TNFR1, FADD, AIFM, CASP8, TP53, DFFA and NFKB1. These genes are predicted to mediate apoptotic cell death via both intrinsic and extrinsic apoptosis pathway. In summary, we report the identification of new N-feruloyltyramine derivatives from alkaloid extract of T. terristris fruit with probable anti-leukemic and pharmacological potential.
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Integrating Ultrasound with Activated Carbon prepared from Mangosteen Fruit Peel Waste Material for Effective Removal of Reactive Black 5 dye(Environmental Engineering and Management Journal)
(Randeep Singh, P.V. Nidheesh, T. Sivasankar,,,,Year : 2019)
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No information is available
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Treatment of low strength wastewater using compact submerged aerobic fixed film (SAFF) reactor filled with high specific surface area synthetic media(Water Science & Technology)
(Rishi Gurjar, Akshay Shende, Girish Pophali,,,,Year : 2019)
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No information is available
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